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Introduction to Gene Therapy

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Reproductive Endocrinology

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Gene therapy is broadly defined as the delivery or transferof genetic material to target cells for therapeutic purposes. Elucidation of the molecular bases of inherited diseases as well as acquired diseases, such as cancer, allows for the possibility of therapeutic interventions at the molecular level. The therapeutic benefits may be achieved by, interfering with gene function, restoring lost function, or initiating a new function in the target cells. Current methods of gene transfer include the use of viral and non-viral vectors [1–5]. Viral vectors accomplish gene transfer directly by viral-mediated infection.

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Author information

Authors and Affiliations

  1. Department of Obstetrics and Gynecology, Meharry Medical College Center for Women Health Research, Nashville, TN, USA

    Ayman Al-Hendy

  2. Department of Obstetrics & Gynecology, University of Texas Medical Branch, Galveston, TX, USA

    Salama A. Salama

Authors
  1. Ayman Al-Hendy
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  2. Salama A. Salama
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Corresponding author

Correspondence to Ayman Al-Hendy .

Editor information

Editors and Affiliations

     

    AAV:

    adeno-associated viruses

    Ad5:

    type of adenovirus

    Ad-ER-DN:

    adenoviral vector that expresses a dominant negative ER mutant

    Ad-LacZ:

    adenovirus expressing the marker gene β-lactamase

    ALDH-1:

    aldehyde dehydrogenase class 1 gene

    bFGF:

    basic fibroblast growth factor

    CaCL2:

    calcium chloride

    CAR:

    coxsackie-adenovirus receptor

    CD/5-FCyt:

    cytosine deaminase plus 5-fluorocytosine

    CMV:

    cytomegalovirus

    CRAds:

    conditionally replicative adenoviruses

    DNA:

    deoxyribonucleic acid

    ELT3:

    cell line derived from Eker rat leiomyoma

    ER:

    estrogen receptor

    ERE:

    estrogen receptor element

    ERI-536:

    estrogen receptor mutant

    GCV:

    ganciclovir

    HSV:

    herpes simplex virus

    HSV-tk:

    herpes simplex virus-thymidine kinase

    LM-15:

    human leiomyoma cell line

    MDR1:

    multidrug resistance gene

    MOI:

    multiplicity of infection

    PDGF:

    platelet-derived endothelial growth factor

    RNA:

    ribonucleic acid

    siRNA:

    small interfering RNA

    TK-GCV:

    thymidine kinase – ganciclovir

    TNF:

    tumor necroses factor

    TRAIL:

    TNF apoptosis-inducing ligand

    VEGF:

    vascular endothelial growth factor

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    Al-Hendy, A., Salama, S.A. (2009). Introduction to Gene Therapy. In: Chedrese, P. (eds) Reproductive Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-88186-7_12

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