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Replication of Murine Mitochondrial DNA Following Irradiation

  • Hengshan Zhang
  • David Maguire
  • Steven Swarts
  • Weimin Sun
  • Shanmin Yang
  • Wei Wang
  • Chaomei Liu
  • Mei Zhang
  • Di Zhang
  • Louie Zhang
  • Kunzhong Zhang
  • Peter Keng
  • Lurong Zhang
  • Paul Okunieff
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 645)

Abstract

The effect of radiation on the mitochondrial genome in vivois largely unknown. Though mitochondrial DNA (mtDNA) is vital for cellular survival and proliferation, it has little DNA repair machinery compared with nuclear DNA (nDNA). A better understanding of how radiation affects mtDNA should lead to new approaches for radiation protection. We have developed a new system using real-time PCR that sensitively detects the change in copy number of mtDNA compared with nDNA. In each sample, the DNA sequence coding 18S rRNA served as the nDNA reference in a run simultaneously with a mtDNA sequence. Small bowel collected 24 hours after 2 Gy or 4 Gy total body irradiation (TBI) exhibited increased levels of mtDNA compared with control mice. A 4 Gy dose produced a greater effect than 2 Gy. Similarly, in bone marrow collected 24 hours after 4 Gy or 7 Gy TBI, 7 Gy produced a greater response than 4 Gy. As a function of time, a greater effect was seen at 48 hours compared with 24 hours. In conclusion, we found that radiation increased the ratio of mtDNA:nDNA and that this effect seems to be tissue independent and seems to increase with radiation dose and duration following radiation exposure.

Keywords

Small Bowel Total Body Irradiation Double Strand Break Repair Relative Copy Number Irradiate Bone Marrow 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hengshan Zhang
  • David Maguire
  • Steven Swarts
  • Weimin Sun
  • Shanmin Yang
  • Wei Wang
  • Chaomei Liu
  • Mei Zhang
  • Di Zhang
  • Louie Zhang
  • Kunzhong Zhang
  • Peter Keng
  • Lurong Zhang
  • Paul Okunieff
    • 1
  1. 1.Department of Radiation OncologyUniversity of Rochester Medical CenterUSA

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