Myocardial Capillary Net And Blood Constituents In Streptozotocin (Stz)-Induced Diabetic Rats

  • Tomiyasu Koyama
  • Akira Taka
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 645)


Type 1 diabetes was induced in Wistar rats by injection of streptozotocin (STZ). Changes in the myocardial capillary network were examined using the doublestaining enzymatic method for alkaline phosphatase (AP) and dipeptidylpeptidase IV (DPPIV) This method allows the identification of the arteriolar (AP-containing) and the venular (DPPIV-containing) portions of the capillary network. In addition, blood plasma was analysed. The AP- and AP/DPPIV-containing capillary portions increased significantly, accompanied by a decrease in the DPPIV-containing portions in 60 days. A significant increase in AP was observed in the plasma. The capillary domain areas of each capillary portion were larger in the STZ-injected group than in the controls. It appears that oxygen transport to the subendocardial myocardial tissues may be decreased in the STZ group. In rats fed with Saji-supplemented chow there was a decrease in plasma AP, with increases in hemoglobin, hematocrit and vitamin C, suggesting a partial improvement of metabolic function and oxygen supply in these diabetic Wistar rats.


Oxygen Transport Capillary Network Blood Constituent Hippophae Rhamnoides Abdominal Vein 
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© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tomiyasu Koyama
    • 1
  • Akira Taka
    • 2
  1. 1.Hokkaido UniversityuSapporoJapan
  2. 2.Sapporo-Aoba School of Holistic MedicineSapporoJapan

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