Non-Invasive Measurement Of the Superficial Cortical Oxygen Partial Pressure

  • Chris Woertgen
  • Jan Warnat
  • Alexander Brawanski
  • Georg Liebsch
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 645)


We present a non invasive fluorescein based method to measure and visualise the partial oxygen pressure of the rat cortex in a 2D picture. We studied 10 Wistar rats. A trepanation was done over the hemisphere and the dura was opened. A PMMA cylinder with a calibrated optical membrane was fixed over the surface of the brain. The CCD camera with the light source is placed over the cylinder. This allows the generation of two-dimensional maps of the pO2 pressure. Using the white light picture we defined regions of interest (ROI) in an artery, vein, parenchyma and an overall ROI. For every ROI a mean emission value was calculated. We increased, stepwise, the FiO2 from 30% up to 100%. Thereafter we established ventilation with an FiO2 of 30% and induced a stepwise hypo- and hyperventilation. The ROI‘s showed significantly different pO2 values. The apO2 showed a good correlation to the pO2 in the ROIs. This new set up seems to give reliable absolute pO2 values of the brain surface. This method seems to be able for the first time to give a non invasive pO2 map of the brain surface reflecting oxygenation and ventilation effects.


Brain Surface Luminescence Lifetime Colour Picture Pairwise Multiple Comparison Procedure Arterial Blood Pressure Measurement 
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  1. 1.
    G. Liebsch, I. Klimant, C. Krause, OS. Wolfbeis, Fluorescent imaging of pH with optical sensors using timedomain dual lifetime referencing, Anal. Chem 73, 354-4363 (2001).CrossRefGoogle Scholar
  2. 2.
    G. Liebsch, I. Klimant, B. Frank, G. Holst, OS. Wolfbeis, Luminescence Lifetime Imaging of Oxygen, pH, and Carbon Dioxide Distribution Using Optical Sensors, Applied Spectroscopy 54, 548-59 (2000).CrossRefGoogle Scholar
  3. 3.
    P. Babilas, G. Liebsch, V. Schacht, I. Klimant, OS. Wolfbeis RM. Szeimies, C. Abels, In vivo phosphorescence imaging of pO2 using planar oxygen sensors. Microcirculation 12, 477-87 (2005).PubMedCrossRefGoogle Scholar
  4. 4.
    G. Holst, O. Kohls, I. Klimant, B. König, M. Kühl, T. Richter, A modular luminescence lifetime imagingsystem for mapping oxygen distribution in biological samples. Sensors and Actuators 51, 163-70 (1998).CrossRefGoogle Scholar
  5. 5.
    A.J. Johnston, L.A. Steiner, A.K. Gupta, D.K. Menon, Cerebral oxygen vasoreactivity and cerebral oxygenreactivity, British Journal of Anaesthesia 90, 774-786, (2003).PubMedCrossRefGoogle Scholar
  6. 6.
    D.W. Lübbers, H. Baumgärtl, Heterogeneities and profiles of oxygen pressure in brain and kidney as examples of the pO2 distribution in the living tissue, Kidney International, 51, 372-380, (1997).PubMedCrossRefGoogle Scholar
  7. 7.
    J.C. Rose, T.A. Neill, J.C. Hemphil III, Continuous monitoring of the microcirculation in neurocritical care: an update on brain tissue oxygenation, Current Opinion in Critical Care 12, 97-102, (2006).Google Scholar
  8. 8.
    E. Vovenko, Distribution of oxygen tension on the surface of arterioles, capillaries and venules of brain cortex and in tissue in normoxia: an experimental study on rats, Pflügers Arch-Eur J Physiol 437, 617-623, (1999).CrossRefGoogle Scholar
  9. 9.
    M.J. Purves, The Physiology of the Cerebral Circulation, (Cambridge University Press, 1972).Google Scholar
  10. 10.
    M. Menzel, E.M.R. Doppenberg, A. Zauner, J. Soukoup, D. Henze, T. Clausen, A. Rieger, R. Bullock, J. Radke, Oxygen reactivity in patients after severe head injury – a prognostic tool ? Zentralblatt für Neurochirurgie 61, 181-187, (2000).PubMedCrossRefGoogle Scholar
  11. 11.
    K.P. Ivanov, I.B. Sokolova, E.P. Vovenko, Oxygen transport in the rat brain cortex at normobaric hyperoxia. Eur J Appl Physiol 80, 582-587, (1999).CrossRefGoogle Scholar
  12. 12.
    B.R. Duling, W. Kushinsky, M. Wahl, Measurements of the perivascular PO2 in the vicinity of the pial vessels of the cat. Pflügers Arch 383, 29-34, (1979).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Chris Woertgen
    • 1
  • Jan Warnat
    • 1
  • Alexander Brawanski
    • 1
  • Georg Liebsch
    • 2
  1. 1.Dept. of NeurosurgeryUniversity of RegensburgGermany
  2. 2.Biocam GmbHGermany

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