Tropomyosin pp 232-249 | Cite as

Tropomyosin and ADF/Cofilin as Collaborators and Competitors

  • Thomas B. Kuhn
  • James R. Bamburg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 644)


Dynamics of actin filaments is pivotal to many fundamental cellular processes such as cytokinesis, motility, morphology, vesicle and organelle transport, gene transcription and senescence. In vivo kinetics of actin filament dynamics is far from the equilibrium in vitro and these profound differences are attributed to large number of regulatory proteins. In particular, proteins of the ADF/cofilin family greatly increase actin filament dynamics by severing filaments and enhancing depolymerization of ADP-actin monomers from their pointed ends. Cofilin binds cooperatively to a minor conformer of F-actin in which the subunits are slightly under rotated along the filament helical axis. At high stoichiometry of cofilin to actin subunits, cofilin actually stabilizes actin filaments. Many isoforms of tropomyosin appear to compete with ADF/cofilin proteins for binding to actin filaments. Tropomyosin isoforms studied to date prefer binding to the “untwisted” conformer of F-actin and through their protection and stabilization of F-actin, recruit myosin II and assemble, different actin superstructures from the cofilin-actin filaments. However, some tropomyosin isoforms may synergize with ADF/cofilin to enhance filament dynamics, suggesting that the different isoforms of tropomyosins, many of which show developmental or tissue specific expression profiles, play major roles in the assembly and turnover of actin superstructures. Different actin superstructures can overlap both spatially and temporally within a cell, but can be differentiated from each other based upon their kinetic and kinematic properties. Furthermore, local regulation of ADF/cofilin activity through signal transduction pathways could be one mechanism to alter the dynamic balance in F-actin-binding of certain tropomyosin isoforms in subcellular domains.


Actin Dynamic Actin Monomer Body Wall Muscle Cleavage Furrow Actin Cable 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Chick JK, Lindberg U, Schutt CE. The structure of an open state of beta-actin at 2.65A resolution. J. Mol Biol 1996; 263:607–623.CrossRefGoogle Scholar
  2. 2.
    Otterbein LR, Graceffa, P, Dominguez R. The crystal structure of uncomplexed actin in the ADP state. Science 2001; 293:708–711.PubMedCrossRefGoogle Scholar
  3. 3.
    Holmes KC, Popp D, Gebhard W et al. Atomic model of the actin filament. Nature 1990; 347:44–49.PubMedCrossRefGoogle Scholar
  4. 4.
    Lorenz M, Popp D, Holmes KC. Refinement of the F-actin model against X-ray fiber diffraction data by the use of a directed mutation algorithm. J Mol Biol 1993; 234:826–836.PubMedCrossRefGoogle Scholar
  5. 5.
    Pollard TD, Blanchoin L, Mullins RD. Molecular mechanisms controlling actin filament dynamics in nonmuscle cells. Annu Rev Biophys Biomol Struct 2000; 29:545–576.PubMedCrossRefGoogle Scholar
  6. 6.
    Carlier MF, Laurent V, Santolini L et al. Actin depolymerizing factor (ADF/cofilin) enhances the rate of filament turnover: implication in actin-based motility. J Cell Biol 1997; 136:1307–1322.PubMedCrossRefGoogle Scholar
  7. 7.
    Carlier MF, Pantaloni D. Control of actin dynamics in cell motility. J Mol Biol 1997; 269: 459–467.PubMedCrossRefGoogle Scholar
  8. 8.
    Bamburg JR. Proteins of the ADF/Cofilin family: essential regulators of actin dynamcs. Annu Rev Cell Dev Biol 1999; 15:185–230.PubMedCrossRefGoogle Scholar
  9. 9.
    Moseley JB, Goode BL. The yeast actin cytoskeleton: from cellular function to biochemical mechanism. Microbiol Mol Biol Rev 2006; 70:605–645.PubMedCrossRefGoogle Scholar
  10. 10.
    Hehnly H, Stamnes M. Regulating cytoskeleton-based motility. FEBS Lett 2007; 581:2112–2118.PubMedCrossRefGoogle Scholar
  11. 11.
    Pollard TD. Cellular motility powered by actin filament assembly and disassembly. Harvey Lect 2002–2003; 98:1–17.PubMedGoogle Scholar
  12. 12.
    Obrdlik A, Kukalev A, Percipalle P. The function of actin in gene transcription. Histol Histopathol 2007; 22:1051–1055.PubMedGoogle Scholar
  13. 13.
    Pak C, Flynn KC, Bamburg JR. Actin binding proteins take the reigns in growth cones. Nature Revs Neurosci 2008; 9(2):136–147.CrossRefGoogle Scholar
  14. 14.
    Gunning PW, Schevzov G, Kee AJ et al. Tropomysoin isoforms: divining rods for actin cytoskeleton function. Trends Cell Biol 2005; 15(6):333–341.PubMedCrossRefGoogle Scholar
  15. 15.
    Lappalainen P, Kessels MM, Cope MJTV. The ADF homology (ADF-H) domain: a highly exploited actin-binding module. Mol Biol Cell 1998; 9:1951–1959.PubMedGoogle Scholar
  16. 16.
    Puius YA, Mahoney NM, Almo SC. The modular structure of actin-regulatory proteins. Curr Opin Cell Biol 1998; 10:23–34.PubMedCrossRefGoogle Scholar
  17. 17.
    Vartiainen MK, Mustonen T, Mattila PK et al. The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type specific requirements for actin dynamics. Mol Biol Cell 2002; 13:183–194.PubMedCrossRefGoogle Scholar
  18. 18.
    Thirion C, Stucka R, Mendel B et al. Characterization of human muscle type cofilin (CFL2) in normal and regenerating muscle. Eur J Biochem 2001; 268:3473–3482.PubMedCrossRefGoogle Scholar
  19. 19.
    Abe H, Nagaoka R, Obinata T. Cytoplasmic localization and nuclear transport of cofilin in cultured myotubes. Exp Cell Res 1993; 206:1–10.PubMedCrossRefGoogle Scholar
  20. 20.
    Bamburg JR, McGough A, Ono S. Putting a new twist on actin: ADF/cofilins modulate actin dynamics. Trends Cell Biol 1999; 9:364–370.PubMedCrossRefGoogle Scholar
  21. 21.
    Lappalainen R, Fedorov EV, Fedorov AA et al. Essential functions and actin-binding surfaces of yeast cofilin by systematic mutagenesis. EMBO J 1997; 16:5520–5530.PubMedCrossRefGoogle Scholar
  22. 22.
    Galkin VE, Orlova A, Lukoyanova N et al. Actin depolymerizing factor stabilizes an existing states of F-actin and can change the tilt of F-actin subunits. J Cell Biol 2001; 153:75–86.PubMedCrossRefGoogle Scholar
  23. 23.
    Hawkins M, Pope, B, Maciver SK et al. The interaction of human actin depolymerizing factor with actin is pH regulated. Biochemistry 1993; 32:9985–9993.PubMedCrossRefGoogle Scholar
  24. 24.
    Hayden SM, Miller PS, Brauweiler A et al. Analysis of the interactions of actin depolymerizing factor with G-and F-actin. Biochemistry 1993; 32:9994–10004.PubMedCrossRefGoogle Scholar
  25. 25.
    McCough A, Pope B, Chui W et al. Cofilin changes the twist of F-actin: implications for, actin filament dynamics and cellular function. J Cell Biol 1997; 138:771–781.CrossRefGoogle Scholar
  26. 26.
    Andrianantoandro E, Pollard TD. Mechanism of actin filament turnover by severing and nucleation at different concentrations of ADF/cofilin. Mol Cell 2006; 24:13–23.PubMedCrossRefGoogle Scholar
  27. 27.
    Pavlov D, Muhlrad A, Cooper J et al. Actin filament severing by cofilin. J Mol Biol 2007; 365:1350–1358.PubMedCrossRefGoogle Scholar
  28. 28.
    Blanchoin L, Pollard TD, Mullin RD. Interactions of ADF/cofilin, Arp2/3 complex, capping proteins and profilin in remodeling of branched actin filament networks. Curr Biol 2000; 10:1273–1282.PubMedCrossRefGoogle Scholar
  29. 29.
    Moriyama K, Yahara I. Two activities of cofilin, severing and accelerating depolymerization of actin filaments, are affected differentially, by mutations around the actin-binding helix. EMBO J 1999; 18:6752–6761.PubMedCrossRefGoogle Scholar
  30. 30.
    Chen H, Bernstein BW, Sneider JM et al. In vitro activity differences between proteins of the ADF/cofilin family define two distinct subgroups. Biochemistry 2004; 43:7127–7142.PubMedCrossRefGoogle Scholar
  31. 31.
    Yeoh S, Pope B, Mannherz HG et al. Determining the differences in actin binding by human ADF and cofilin. J Mol Biol 2002; 315:911–925.PubMedCrossRefGoogle Scholar
  32. 32.
    Nishida E. Opposite effects of cofilin and profilin from porcine brain on rate of exchange of actin-bound adenosine 5’-triphosphate. Biochemistry 1985; 24:1160–1164.PubMedCrossRefGoogle Scholar
  33. 33.
    Moriyama K, Yahara I. Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover. J Cell Sci 2002; 115:1591–1601.PubMedGoogle Scholar
  34. 34.
    Bertling E, Hotulainen P, Mattila PK et al. Cyclase associated protein 1 (CAP1) promotes cofilin-induced actin dynamics in mammalian nonmuscle cells. Mol Biol Cell 2004; 15:2324–2334.PubMedCrossRefGoogle Scholar
  35. 35.
    Paavilainen VO, Bertling E, Falck S et al. Regulation of cytoskeletal dynamics by actin-monomer binding proteins. Trends Cell Biol 2004; 14:386–394.PubMedCrossRefGoogle Scholar
  36. 36.
    Bertling E, Quintero-Monzon O, Mattila PK et al. Mechanism and biological role of profilin-Srv2/CAP interaction. J Cell Sci 2007; 120:1225–1234.PubMedCrossRefGoogle Scholar
  37. 37.
    Agnew BJ, Minamide LS, Bamburg JR. Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site. J Biol Chem 1995; 270:17582–17587.PubMedCrossRefGoogle Scholar
  38. 38.
    Arber S, Barbayaniis FA, Hanser H et al. Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase. Nature 1998; 393:805–809.PubMedCrossRefGoogle Scholar
  39. 39.
    Yang N, Higuchi O, Ohashi K et al. Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 1998; 393:809–812.PubMedCrossRefGoogle Scholar
  40. 40.
    LaLonde DP, Brown MC, Bouverat BP et al. Actopaxin interacts with TESK1 to regulate cell spreading on fibronectin. J Biol Chem 2005; 280:21680–21688.PubMedCrossRefGoogle Scholar
  41. 41.
    Niwa R, Nagata-Ohashi K, Takeichi M et al. Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. Cell 2002; 108:233–246.PubMedCrossRefGoogle Scholar
  42. 42.
    Huang TY, DerMardirossian C, Bokoch GM. Cofilin phosphatases and regulation of actin dynamics. Curr Opin Cell Biol 2006; 18:26–31.PubMedCrossRefGoogle Scholar
  43. 43.
    Nishita M, Tomizawa C, Yamamoto M et al. Spatial and temporal regulation of cofilin activity by LIM kinase and Slingshot is critical for directional cell migration. J Cell Biol 2005; 171:349–359.PubMedCrossRefGoogle Scholar
  44. 44.
    Yonezawa N, Nishida E, Iida K et al. Inhibition of the interactions of cofilin, destrin and deoxyribonuclease I with actin by phosphoinositides. J Biol Chem 1990; 265:8382–8386.PubMedGoogle Scholar
  45. 45.
    Gorbatyuk VY, Nosworthy NJ, Robson SA et al. Mapping the phosphoinositide-binding site on chick cofilin explains how PIP2 regulates the cofilin-actin interaction. Mol Cell 2006; 24:511–522.PubMedCrossRefGoogle Scholar
  46. 46.
    Okada K, Blanchoin L, Abe H et al. Xenopus actin interacting protein (XAip1) enhances cofilin fragmentation of filaments by capping filament ends. J Biol Chem 2002; 277:43011–43016.PubMedCrossRefGoogle Scholar
  47. 47.
    Ono S. Regulation of actin filament dynamics by actin depolymerizing factor/cofilin and actin-interacting protein 1: new blades for twisted filaments. Biochemistry 2003; 42:13363–13370.PubMedCrossRefGoogle Scholar
  48. 48.
    Okada K, Ravi H, Smith EM et al. Aip 1 and cofilin promote rapid turnover of yeast actin patches and cables: a coordinated mechanism for severing and capping filaments. Mol Biol Cell 2006; 17:2855–2868.PubMedCrossRefGoogle Scholar
  49. 49.
    Clark MG, Amberg DC. Biochemical and genetic analyses provide insight into the structural and mechanistic properties of actin filament disassembly by the Aip1 cofilin complex in Saccharomyces cerevisia. Genetics 2007; 176:1527–2539.PubMedCrossRefGoogle Scholar
  50. 50.
    Hotulainen P, Paunola E, Vartiainen MK et al. Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. Mol Biol Cell 2005; 16:649–664.PubMedCrossRefGoogle Scholar
  51. 51.
    Minamide LS, Painter WB, Schevzov G et al. Differential regulation of actin depolymerizing factor and cofilin in response to alterations in the actin monomer pool. J Biol Chem 1997; 272:8303–8309.PubMedCrossRefGoogle Scholar
  52. 52.
    Bernstein BW, Painter WB, Chen H et al. Intracellular pH modulation of ADF/cofilin proteins. Cell Motil Cytoskeleton 2000; 47:319–336.PubMedCrossRefGoogle Scholar
  53. 53.
    Estornes Y, Gay F, Gevrey JC et al. Differential involvement of destrin and cofilin-1 in the control of invasive properties of Isrecol human colon cancer cells. Int J Cancer 2007; 121:2162–2171.PubMedCrossRefGoogle Scholar
  54. 54.
    Bernstein BW, Bamburg JR. Tropomyosin binding to F-actin protects the F-actin from disassembly by brain actin-depolymerizing factor (ADF). Cell Motil 1982; 2:1–8.PubMedCrossRefGoogle Scholar
  55. 55.
    Bamburg JR, Bernstein BW. Actin and actin-binding proteins in neurons. In: Burgoyne RD, ed. The Neuronal Cytoskeleton. New York: Wiley-Liss, 1991:121–160.Google Scholar
  56. 56.
    Nishida E, Maekawa S, Sakai H. Cofilin, a protein in porcine brain that binds to actin filaments and inhibits their interactions with myosin and tropomyosin. Biochemistry 1984; 23:5307–5317.PubMedCrossRefGoogle Scholar
  57. 57.
    McCough A. F-actin binding proteins. Curr Opin Struct Biol 1998; 8:166–167.CrossRefGoogle Scholar
  58. 58.
    Lal AA, Korn ED. Effect of tropomyosin on the kinetics of polymerization of muscle actin. Biochemistry 1986; 25:1154–1158.PubMedCrossRefGoogle Scholar
  59. 59.
    Blanchoin L, Pollard TD, Hitchock-DeGregori SE. Inhibition of the Arp2/3 complex-nucleated actin polymerization and branch formation by tropomyosin. Curr Biol 2001; 11:1300–1304.PubMedCrossRefGoogle Scholar
  60. 60.
    Weinberger R, Schevzov G, Jeffrey P et al. The molecular composition of neuronal microfilaments is spatially and temporally regulated. J Neurosci 1996; 16:238–252.PubMedGoogle Scholar
  61. 61.
    Bryce NS, Schevzov G, Ferguson V et al. Specification of actin filament function and molecular composition by tropomyosin isoforms. Mol Biol Cell 2003; 14:1002–1016.PubMedCrossRefGoogle Scholar
  62. 62.
    Stehn JR, Schevzov G, O’Neill GM et al. Specialization of the tropomyosin composition of actin filaments provides new potential targets for chemotherapy. Curr Cancer Drug Targets 2006; 6:245–256.PubMedCrossRefGoogle Scholar
  63. 63.
    Pittenger MF, Helfman DM. In vitro and in vivo characterization of four fibroblast tropomyosins produced in bacteria: TM-2, TM-3, TM-5a and TM-5b are colocalized in interphase fibroblasts. J Cell Biol 1992: 118:841–858.PubMedCrossRefGoogle Scholar
  64. 64.
    Yu R, Ono S. Dual roles of tropomyosin as an F-actin stabilizer and a regulator of muscle contraction in Caenorhabditis Elagans body wall muscle. Cell Motil Cytoskeleton 2006; 63:659–672.PubMedCrossRefGoogle Scholar
  65. 65.
    Liu HP, Bretscher A. Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell 1989; 57:233–242.PubMedCrossRefGoogle Scholar
  66. 66.
    Moon AL, Janmey PA, Louie KA et al. Cofilin is an essential component of the yeast cortical cytoskeleton. J Cell Biol 1993; 120:421–435.PubMedCrossRefGoogle Scholar
  67. 67.
    Wen K-K, Kuang B, Rubenstein PA. Tropomyosin-dependent filament formation by a polymerizationdefective mutant yeast actin (V266G, L267G). J Biol Chem 2000; 275:40594–40600.PubMedCrossRefGoogle Scholar
  68. 68.
    Bharadwaj S, Hitchcock-DeGregori S, Thorburn A et al. N terminus is essential for tropomyosin functions: N-terminal modification disrupts stress fiber organization and abolishes anti-oncogenic effects of tropomyosin-1. J Biol Chem 2004; 279:14039–14048.PubMedCrossRefGoogle Scholar
  69. 69.
    McKim KS, Matheson C, Marra MA et al. The Caenorhabditis elegans unc-60 gene encodes proteins homologous to a family of actin-binding proteins. Mol Gen Genet 1994; 242:346–357.PubMedCrossRefGoogle Scholar
  70. 70.
    Ono S, Baillie DL, Benian GM. UNC-60B, an ADF/cofilin family protein, is required for proper assembly of actin into myofibrils in Caenorhabditis elegans body wall muscle. J Cell Biol 1999; 145:491–502.PubMedCrossRefGoogle Scholar
  71. 71.
    Ono S, Ono K. Tropomyosin inhibits ADF/cofilin-dependent actin filament dynamics. J Cell Biol 2002; 156:1065–1076.PubMedCrossRefGoogle Scholar
  72. 72.
    Ono S. Regulation of actin filament dynamics by actin depolymerizing factor/cofilin and actin-interacting protein 1: New blades for twisted filaments. Biochemistry 2003; 42:13363–13370.PubMedCrossRefGoogle Scholar
  73. 73.
    Schevzov G, Gunning P, Jeffrey PL et al. Tropomyosin localization reveals distinct populations of microfilaments in neurites and growth cones. Mol Cell Neurosci 1997; 8:439–454.PubMedCrossRefGoogle Scholar
  74. 74.
    Schevzov G, Bryce NS, Almonte-Baldonado R et al. Specific features of neuronal size and shape are regulated by tropomyosin isoforms. Mol Cell Biol 2005; 16:3425–3437.CrossRefGoogle Scholar
  75. 75.
    Cooper J. Actin dynamics: tropomyosin provides stability. Curr Biol 2002; 12:R523–525.PubMedCrossRefGoogle Scholar
  76. 76.
    Balasubramanian MK, Helfman DM, Hemmingsen SM. A new tropomyosin essential for cytokinesis in the fission yeast S. pombe. Nature 1992; 360:84–87.PubMedCrossRefGoogle Scholar
  77. 77.
    Lin JJ, Warren KS, Wamboldt DD et al. Tropomyosin isoforms in nonmuscle cells. Int Rev Cytol 1997; 170:1–38.PubMedCrossRefGoogle Scholar
  78. 78.
    Hughes JA, Cooke-Yarborough CM, Chadwick NC et al. High molecular weight tropomyosins localise to the contractile rings of dividing CNS cells but are absent from malignant paediatric and adult CNS tumours. GLIA 2003; 42:25–35.PubMedCrossRefGoogle Scholar
  79. 79.
    Percival JM, Thomas G, Cock TA et al. Sorting of tropomyosin isoforms in synchronised NIH 3T3 fibroblasts: evidence for distinct microfilament populations. Cell Motil Cytoskeleton 2000; 47:189–208.PubMedCrossRefGoogle Scholar
  80. 80.
    Schevzov G, Vrhovski B, Bryce NS et al. Tissue-specific tropomyosin isoform composition. J Histochem Cytochem 2005; 53:557–570.PubMedCrossRefGoogle Scholar
  81. 81.
    Glotzer M. Animal cell cytokinesis. Annu Rev Cell Dev Biol 2001; 17:351–386.PubMedCrossRefGoogle Scholar
  82. 82.
    Noguchi T, Mabuchi I. Reorganization of actin cytoskeleton at the growing end of the cleavage furrow of Xenopus egg during cytokinesis. J Cell Sci 2001; 114:401–412.PubMedGoogle Scholar
  83. 83.
    Pelham RJ, Chang F. Actin dynamics in the contractile ring during cytokinesis in fission yeast. Nature 2002; 419:82–86.PubMedCrossRefGoogle Scholar
  84. 84.
    Marks J, Hyams JS. Localization of F-actin through the cell division cycle of Schizosaccharomyces pombe. Eur J Cell Biol 1985; 39:27–32.Google Scholar
  85. 85.
    Arai R, Nakano K, Mabuchi I. Subcellular localization and possible function of actin, tropomyosin and actin-related protein 3 (Arp3) in the fission yeast Schizosaccharomyces pombe. Eur J Cell Biol 1998; 76:288–295.PubMedGoogle Scholar
  86. 86.
    Arai R, Mabuchi I. F-actin ring formation and the role of F-actin cables in the fission yeast Schizosaccharomyces pombe. J Cell Sci 2002; 115:887–898.PubMedGoogle Scholar
  87. 87.
    Nagaoka R, Abe H, Kusano K. Concentration of cofilin, a small actin-binding protein, at the cleavage furrow during cytokinesis. Cell Motil Cytoskeleton 1995; 30:1–7.PubMedCrossRefGoogle Scholar
  88. 88.
    Balasubramanian MK, Hirani BR, Burke JD et al. The Schizosaccharomyces pombe cdc3+ gene encodes a profilin essential for cytokinesis. J Cell Biol 1994; 125:1289–1301.PubMedCrossRefGoogle Scholar
  89. 89.
    Gunsalus KC, Bonaccorsi S, Williams E et al. Mutations in twinstar, a Drosophila gene encoding a cofilin/ADF homologue, result in defects in centrosome migration and cytokinesis. J Cell Biol 1995; 131:1243–1259.PubMedCrossRefGoogle Scholar
  90. 90.
    Abe H, Obinata T, Minamide LS et al. Xenopus laevis actin depolymerizing factor/cofilin: a phosphorylation-regulated protein essential for development. J Cell Biol 1996; 132:871–885.PubMedCrossRefGoogle Scholar
  91. 91.
    Balasubramanian MK, Bi E, Glotzer M. Comparative analysis of cytokinesis in budding yeast, fission yeast and animal cells. Curr Biol 2004; 14:806–818.CrossRefGoogle Scholar
  92. 92.
    Nakano K, Mabuchi I. Actin-depolymerizing protein Adf1 is required for formation and maintenance of the contractile ring during cytokinesis in fission yeast. Mol Biol Cell 2006; 17:1933–1945.PubMedCrossRefGoogle Scholar
  93. 93.
    DesMarais V, Ichetovkin I, Condeelis J et al. Spatial regulation of actin dynamics: a tropomyosin-free, actin-rich compartment at the leading edge. J Cell Sci 2002; 115:4649–4660.PubMedCrossRefGoogle Scholar
  94. 94.
    Gupton SL, Anderson KL, Kole TP et al. Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin. J Cell Biol 2005; 168:619–631.PubMedCrossRefGoogle Scholar
  95. 95.
    Dawe HR, Minamide LS, Bamburg JR et al. ADF/cofilin controls cell polarity during fibroblast migration. Curr Biol 2003; 13:252–257.PubMedCrossRefGoogle Scholar
  96. 96.
    Bakin AV, Safina A, Rinehart C et al. A critical role of tropomyosins in TGF-beta regulation of the actin cytoskeleton and cell motility in epithelial cells. Mol Biol Cell 2004; 15:4682–4694.PubMedCrossRefGoogle Scholar
  97. 97.
    Danuser G. Coupling the dynamics of two actin networks—new views on the mechanics of cell protrusion. Biochem Soc Trans 2005; 33:1250–1253.PubMedCrossRefGoogle Scholar
  98. 98.
    Iwasa JH, Mullins RD. Spatial and temporal relationships between actin filament nucleation, capping and disassembly. Curr Biol 2007; 17:395–406.PubMedCrossRefGoogle Scholar
  99. 99.
    Delorme V, Machacek M, DerMardirossian C et al. Cofilin activity downstream of Pak1 regulates cell protrusion effciency by organizing lamellipodium and lamella actin networks. Developmental Cell 2007; 13:646–662.PubMedCrossRefGoogle Scholar
  100. 100.
    Ikonen E, de Almeida JB, Fath KR et al. Myosin II is associated with Golgi membranes: identification of p200 as nonmuscle myosin II on Golgi-derived vesicles. J Cell Sci 1997; 110:2155–2164.PubMedGoogle Scholar
  101. 101.
    Heimann K, Percival JM, Weinberger R et al. Specific isoforms of actin-binding proteins on distinct populations of Golgi-derived vesicles. J Biol Chem 1999; 274:10743–10750.PubMedCrossRefGoogle Scholar
  102. 102.
    Lorra C, Huttner WB. The mesh hypothesis of Golgi dynamics. Nat Cell Biol 1999; 1:E113–E115.PubMedCrossRefGoogle Scholar
  103. 103.
    Pruyne DW, Schott DH, Bretscher A. Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J Cell Biol 1998; 143:1931–1945.PubMedCrossRefGoogle Scholar
  104. 104.
    Rosso S, Bollati F, Bisbal M et al. LIMK1 regulates Golgi dynamics, traffic of Golgi-derived vesicles and process extension in primary cultured neurons. Mol Biol Cell 2004; 15:3433–3449.PubMedCrossRefGoogle Scholar
  105. 105.
    Gourlay CW, Carpp LN, Timpson P. A role for the actin cytoskeleton in cell death and aging in yeast. J Cell Biol 2004; 164:803–809.PubMedCrossRefGoogle Scholar
  106. 106.
    Xu X, Forbes JG, Colombini M. Actin modulates the gating of Neurospora crassa VDAC. J Membr Biol 201; 180:73–81.Google Scholar
  107. 107.
    Maloney MT, Bamburg JR. Cofilin-mediated neurodegeneration in alzheimer’s disease and other amyloidopathies. Mol Neurobiol 2007; 35:21–44.PubMedCrossRefGoogle Scholar
  108. 108.
    Roman I, Figys J, Steurs G et al. Direct measurement of VDAC-actin interaction by surface plasmon resonance. Biochim Biophys Acta 2006; 1758:479–486.PubMedCrossRefGoogle Scholar
  109. 109.
    Chua BT, Volbracht C, Tan KO et al. Mitochondrial translocation of cofilin is an early step in apoptosis induction. Nat Cell Biol 2003; 5:1083–1089.PubMedCrossRefGoogle Scholar
  110. 110.
    Scherthan H. Telomere attachment and clustering during meiosis. J Cell Biol 2005; 170:213–223.PubMedCrossRefGoogle Scholar
  111. 111.
    Trelles-Stricken E, Adelfalk C, Loidl J et al. Meiotic teomere clustering requires actin for its formation and cohesin for its resolution. Nat Cell Biol 2004; 6:1165–1172.CrossRefGoogle Scholar
  112. 112.
    Percipalle P, Visa N. Molecular functions of nuclear actin in transcription. J Cell Biol 2006; 172:967–971.PubMedCrossRefGoogle Scholar
  113. 113.
    Miralles F, Visa N. Actin in transcription and transciption regulation. Curr Op Cell Biol 2006; 18:261–266.PubMedCrossRefGoogle Scholar
  114. 114.
    Hofmann WA, de Lanerolle P. Nuclear actin: to polymerize or not to polymerize. J Cell Biol 2006; 172:541–542.CrossRefGoogle Scholar
  115. 115.
    Wu JI, Crabtree GR. Nuclear actin as choreographer of cell morphology and transcription. Science 2007; 316:1710–1711.PubMedCrossRefGoogle Scholar
  116. 116.
    Vartiainen MK, Guettler S, Larijani B et al. Nuclear actin regulates dynamic subcellular localization and activity of the SRF cofactor MAL. Science 2007; 316:1749–1752.PubMedCrossRefGoogle Scholar

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© Landes Bioscience and Springer Science+Business Media 2008

Authors and Affiliations

  1. 1.Department of ChemistryUniversity of Alaska FairbanksFairbanksUSA
  2. 2.Department of Biochemistry and Molecular BiologyColorado State UniversityFort CollinsUSA

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