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Metabolite Channeling and Multi-enzyme Complexes

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Plant-derived Natural Products

Abstract

The assembly of cooperating enzymes into multicatalytic complexes, also known as “metabolons,” has become a well-accepted concept in cellular metabolism, at least in principle. There are still relatively few examples where the existence of such systems is supported by solid experimental evidence and even fewer where there is evidence for “channeling” of metabolites through the complex. However, proteomic approaches are providing new evidence for the pervasiveness of this type of organization, while structural biology is offering insights into how these systems are constructed and regulated. New and improved technologies for analyzing protein interactions and assemblies, both in vitro and in intact cells, are opening the doors to explo-ring the intracellular organization of a growing number of metabolic complexes in plants and other organisms. There is also an increasing appre-ciation of the surprising scale of many protein interaction networks, the multiple functions of individual proteins, and the importance (and challenges) of compartmentalization. As a result, the concept of enzyme complexes is gaining wider acceptance and becoming an increasingly important consideration in efforts to engineer metabolism.

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Acknowledgments

The author acknowledges the insights of Joe Chappel (on the erg28p system), Danny Kohl (on the implications of recent metabolic profiling experiments in E. coli), and Joe Noel (on the issue of enzyme promiscuity supporting the existence of enzyme complexes), as well as the very helpful comments of three anonymous reviewers. She is grateful to the National Science Foundation for supporting the work in her laboratory on the flavonoid enzyme complex (currently grant number MCB 0445878). This article is dedicated to the memory of H. Olin Spivey, Professor Emeritus of Biochemistry and Molecular Biology at Oklahoma State University, who died on December 5, 2007. A pioneer in the field of metabolic organization, he will be remembered by many as a valued colleague who encouraged newcomers to join the network.

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    Brenda S.J. Winkel

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    Winkel, B.S. (2009). Metabolite Channeling and Multi-enzyme Complexes. In: Osbourn, A., Lanzotti, V. (eds) Plant-derived Natural Products. Springer, New York, NY. https://doi.org/10.1007/978-0-387-85498-4_9

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