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Methods for Molecular Identification of Biosynthetic Enzymes in Plants

  • Sarah O’Connor
Chapter

Abstract

This chapter highlights the major strategies used to identify the genes of plant ­natural product biosynthetic pathways. One or two key examples that illustrate these strategies are provided. A wide range of enzyme classes have been identified using the approaches described in this review including P450 enzymes, terpene systhases, and glycosyltransferases. The advantages and disadvantages of each approach is discussed.

Keywords

Tropane Alkaloid Alkaloid Biosynthesis Tryptophan Decarboxylase Taxol Biosynthesis Plant Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I gratefully acknowledge Dr. Xudong Qu for his helpful comments.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of ChemistryMassachusetts Institute of TechnologyCambridgeUSA

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