Participation of Phytochemicals in Plant Development and Growth

  • Lucille Pourcel
  • Erich Grotewold


Phytochemicals, also known as natural products and specialized compounds, display well known functions in plants providing varying levels of protection to biotic and abiotic stress conditions. The biosynthesis of phytochemicals is tightly spatio-temporally regulated, often restricted to specialized cells, yet their transport within plants allow them to interact with, and modulate, other signalling networks. In this chapter, we describe how phytochemicals participate in plant development and growth, further blurring the boundaries between primary and secondary metabolism, and between hormones and phytochemicals.


Pollen Tube Seed Coat Calorie Restriction Auxin Transport Pollen Germination 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.




Phytohormones are substances that, at low concentration, function to coordinate plant growth and development. The compounds that have been considered as plant hormones include indole-3-acetic acid (auxin), cytokinins, gibberellins (GA), ethylene and abscisic acid (ABA). In addition, brassinosteroids, jasmonic acid (JA) and salicylic acid (SA) have been shown to display important growth regulating activities and are also considered to function as phytohormones.


Interspecies communication of stress signals. This term as been proposed by Howitz and Sinclair [56] to explain the ability of animals and fungi to “sense” and being activated by molecules that are not produced in these organisms, such as phytochemicals.


  1. 1.
    Wink, M. (1999) Introduction. In Functions of Plant Secondary Metabolites and their Exploitation in Biotechnology (Wink, M., ed), 1–16, CRC Press LLC, Boca Raton, FLGoogle Scholar
  2. 2.
    Hammerschmidt, R. (1999) PHYTOALEXINS: What have we learned after 60 years? Annu Rev Phytopathol37, 285–306PubMedCrossRefGoogle Scholar
  3. 3.
    Curir, P. et al (2005) A phytoalexin-like flavonol involved in the carnation (Dianthus caryophyllus)-Fusarium oxysporum f. Sp dianthi pathosystem. J. Phytobiol153, 65–67Google Scholar
  4. 4.
    Bieza, K., and Lois, R. (2001) An Arabidopsis mutant tolerant to lethal ultraviolet-B levels shows constitutively elevated accumulation of flavonoids and other phenolics. Plant Physiol126, 1105–1115PubMedCrossRefGoogle Scholar
  5. 5.
    Seigler, D., and Price, P.W. (1976) Secondary compounds in plants: primary functions. Am Nat110, 101–105CrossRefGoogle Scholar
  6. 6.
    Aharoni, A. et al (2003) Terpenoid metabolism in wild-type and transgenic Arabidopsis plants. Plant Cell15, 2866–2884PubMedCrossRefGoogle Scholar
  7. 7.
    Brown, P.D. et al (2003) Variation of glucosinolate accumulation among different organs and developmental stages of Arabidopsis thaliana. Phytochemistry62, 471–481PubMedCrossRefGoogle Scholar
  8. 8.
    Lepiniec, L. et al (2006) Genetics and biochemistry of seed flavonoids. Annu Rev Plant Biol57, 405–430PubMedCrossRefGoogle Scholar
  9. 9.
    Halkier, B.A., and Gershenzon, J. (2006) Biology and biochemistry of glucosinolates. Annu Rev Plant Biol57, 303–333PubMedCrossRefGoogle Scholar
  10. 10.
    Amme, S. et al (2005) A proteome approach defines protective functions of tobacco leaf trichomes. Proteomicss 5, 2508–2518PubMedCrossRefGoogle Scholar
  11. 11.
    Wienkoop, S. et al (2004) Cell-specific protein profiling in Arabidopsis thaliana trichomes: identification of trichome-located proteins involved in sulfur metabolism and detoxification. Phytochemistry65, 1641–1649PubMedCrossRefGoogle Scholar
  12. 12.
    Bird, D.A., et al. (2003) A tale of three cell types: Alkaloid biosynthesis is localized to sieve elements in opium poppy. Plant Cell15, 2626–2635PubMedCrossRefGoogle Scholar
  13. 13.
    Weid, M. et al (2004) The roles of latex and the vascular bundle in morphine biosynthesis in the opium poppy, Papaver somniferum. Proc Natl Acad Sci USA101, 13957–13962PubMedCrossRefGoogle Scholar
  14. 14.
    Chalker-Scott, L. (1999) Environmental significance of anthocyanins in plant stress responses. Photochem Photobiol70, 1–9CrossRefGoogle Scholar
  15. 15.
    Winkel-Shirley, B. (2002) Biosynthesis of flavonoids and effects of stress. Curr Opin Plant Biol5, 218–223PubMedCrossRefGoogle Scholar
  16. 16.
    Gould, K.S. (2004) Nature’s Swiss army knife: the diverse protective roles of Anthocyanins in leaves. J Biomed Biotechnol2004, 314–320PubMedCrossRefGoogle Scholar
  17. 17.
    Debeaujon, I. et al (2003) Proanthocyanidin-accumulating cells in Arabidopsis testa: regulation of differentiation and role in seed development. Plant Cell15, 2514–2531PubMedCrossRefGoogle Scholar
  18. 18.
    Pourcel, L. et al (2007) Flavonoid oxidation in plants: from biochemical properties to physiological functions. Trends Plant Sci12, 29–36PubMedCrossRefGoogle Scholar
  19. 19.
    Grubb, C.D., and Abel, S. (2006) Glucosinolate metabolism and its control. Trends Plant Sci11, 89–100PubMedCrossRefGoogle Scholar
  20. 20.
    Kiss, J.Z. et al (1996) Gravitropism in roots of intermediate-starch mutants of Arabidopsis. Physiol Plant97, 237–244PubMedCrossRefGoogle Scholar
  21. 21.
    Taylor, L.P., and Grotewold, E. (2005) Flavonoids as developmental regulators. Curr Opin Plant Biol8, 317–323PubMedCrossRefGoogle Scholar
  22. 22.
    Taylor, L.P., and Hepler, P.K. (1997) Pollen germination and tube growth. Annu Rev Plant Physiol Plant Mol Biol48, 461–491PubMedCrossRefGoogle Scholar
  23. 23.
    Guyon, V. et al (2004) Antisense phenotypes reveal a role for SHY, a pollen-specific leucine-rich repeat protein, in pollen tube growth. Plant J39, 643–654PubMedCrossRefGoogle Scholar
  24. 24.
    Shirley, B.W. et al (1995) Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis. Plant J.8, 659–671PubMedCrossRefGoogle Scholar
  25. 25.
    Kitamura, S. et al (2004) TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis. Plant J37, 104–114PubMedCrossRefGoogle Scholar
  26. 26.
    Debeaujon, I. et al (2001) The TRANSPARENT TESTA12 gene of Arabidopsis encodes a multidrug secondary transporter-like protein required for flavonoid sequestration in vacuoles of the seed coat endothelium. Plant Cell13, 853–871PubMedCrossRefGoogle Scholar
  27. 27.
    Hsieh, K., and Huang, A.H. (2007) Tapetosomes in Brassica tapetum accumulate endoplasmic reticulum-derived flavonoids and alkanes for delivery to the pollen surface. Plant Cell19, 582–596PubMedCrossRefGoogle Scholar
  28. 28.
    Mo, Y.Y. et al (1992) Biochemical complementation of chalcone synthase mutants defines a role for flavonols in functional pollen. Proc Natl Acad Sci USA89, 7213–7217PubMedCrossRefGoogle Scholar
  29. 29.
    Blakeslee, J.J. et al (2005) Auxin transport. Curr Opin Plant Biol8, 494–500PubMedCrossRefGoogle Scholar
  30. 30.
    Zazimalova, E. et al (2007) Polar transport of the plant hormone auxin - the role of PIN-FORMED (PIN) proteins. Cell Mol Life Sci64, 1621–1637PubMedCrossRefGoogle Scholar
  31. 31.
    Murphy, A. et al (2000) Regulation of auxin transport by aminopeptidases and endogenous flavonoids. Planta211, 315–324PubMedCrossRefGoogle Scholar
  32. 32.
    Buer, C.S., and Muday, G.K. (2004) The transparent testa4 mutation prevents flavonoid synthesis and alters auxin transport and the response of Arabidopsis roots to gravity and light. Plant Cell16, 1191–1205PubMedCrossRefGoogle Scholar
  33. 33.
    Buer, C.S. et al (2006) Ethylene modulates flavonoid accumulation and gravitropic responses in roots of Arabidopsis. Plant Physiol140, 1384–1396PubMedCrossRefGoogle Scholar
  34. 34.
    Sheahan, J.J., and Rechnitz, G.A. (1992) Flavonoid-specific staining of Arabidopsis thaliana. Biotechniques13, 880–883PubMedGoogle Scholar
  35. 35.
    Mueller, L.A. et al (2000) AN9, a petunia glutathione S-transferase required for anthocyanin sequestration, is a flavonoid-binding protein. Plant Physiol.123, 1561–1570PubMedCrossRefGoogle Scholar
  36. 36.
    Bennett, T. et al (2006) The Arabidopsis MAX pathway controls shoot branching by regulating auxin transport. Current Biology16, 553–563PubMedCrossRefGoogle Scholar
  37. 37.
    Kobayashi, H. et al (2004) Flavanoids induce temporal shifts in gene-expression of nod-box controlled loci in Rhizobium sp. NGR234. Mol Microbiol51, 335–347PubMedCrossRefGoogle Scholar
  38. 38.
    Wasson, A.P. et al (2006) Silencing the flavonoid pathway in Medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia. Plant Cell18, 1617–1629PubMedCrossRefGoogle Scholar
  39. 39.
    Subramanian, S. et al (2007) Distinct, crucial roles of flavonoids during legume nodulation. Trends Plant Sci12, 282–285PubMedCrossRefGoogle Scholar
  40. 40.
    Debeaujon, I. et al (2007) Seed coat development and dormancy. In Seed development, dormancy and germination (Bradford, K. and Nonogaki, H., (editors) Oxford, UK Blackwell Publishing), 25–49Google Scholar
  41. 41.
    Debeaujon, I. et al (2000) Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. Plant Physiol122, 403–413PubMedCrossRefGoogle Scholar
  42. 42.
    Mares, D. et al (2005) A QTL located on chromosome 4A associated with dormancy in white- and red-grained wheats of diverse origin. Theor Appl Genet111, 1357–1364PubMedCrossRefGoogle Scholar
  43. 43.
    Besseau, S. et al (2007) Flavonoid accumulation in Arabidopsis repressed in lignin synthesis affects auxin transport and plant growth. Plant Cell19, 148–162PubMedCrossRefGoogle Scholar
  44. 44.
    Bishopp, A. et al (2006) Signs of change: hormone receptors that regulate plant development. Development133, 1857–1869PubMedCrossRefGoogle Scholar
  45. 45.
    Nugroho, L.H., and Verpoorte, R. (2002) Secondary metabolism in tobacco. Plant Cell Tissue Organ Cult68, 105–125CrossRefGoogle Scholar
  46. 46.
    Siritunga, D. et al (2004) Over-expression of hydroxynitrile lyase in transgenic cassava roots accelerates cyanogenesis and food detoxification. Plant Biotech J2, 37–43CrossRefGoogle Scholar
  47. 47.
    Inderjit, and Duke, S.O. (2003) Ecophysiological aspects of allelopathy. Planta217, 529–539PubMedCrossRefGoogle Scholar
  48. 48.
    Weir, T.L. et al (2004) Biochemical and physiological mechanisms mediated by allelochemicals. Curr Opin Plant Biol7, 472–479PubMedCrossRefGoogle Scholar
  49. 49.
    Bais, H.P. et al (2003) Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science301, 1377–1380PubMedCrossRefGoogle Scholar
  50. 50.
    Blair, A.C. et al (2006) A lack of evidence for an ecological role of the putative allelochemical (+/-)-catechin in spotted knapweed invasion success. J Chem Ecol32, 2327–2331PubMedCrossRefGoogle Scholar
  51. 51.
    Niemeyer, H.M. (1988) Hydroxamic acids (4-Hydroxy-1,4-Benzoxazin-3-Ones), defense chemicals in the Gramineae. Phytochemistry27, 3349–3358CrossRefGoogle Scholar
  52. 52.
    Hejl, A.M., and Koster, K.L. (2004) The allelochemical sorgoleone inhibits root H+-ATPase and water uptake. J Chem Ecology30, 2181–2191CrossRefGoogle Scholar
  53. 53.
    Marvier, M.A. (1996) Parasitic plant-host interactions: plant performance and indirect effects on parasite-feeding herbivores. Ecology77, 1398–1409CrossRefGoogle Scholar
  54. 54.
    Anderson, R.M. et al (2003) Nicotinamide and PNC1 govern lifespan extension by calorie restriction in Saccharomyces cerevisiae. Nature423, 181–185PubMedCrossRefGoogle Scholar
  55. 55.
    Haigis, M.C., and Guarente, L.P. (2006) Mammalian sirtuins - emerging roles in physiology, aging, and calorie restriction. Genes Dev20, 2913–2921PubMedCrossRefGoogle Scholar
  56. 56.
    Howitz, K.T., et al. (2003) Small molecule activators of sirtuins extend Saccharomyces cerevisiae lifespan. Nature425, 191–196PubMedCrossRefGoogle Scholar
  57. 57.
    Lamming, D.W. et al (2004) Small molecules that regulate lifespan: evidence for xenohormesis. Mol Microb53, 1003–1009CrossRefGoogle Scholar
  58. 58.
    Howitz, K.T., and Sinclair, D.A. (2008) Xenohormesis: Sensing the chemical cues of other species. Cell133, 387–391PubMedCrossRefGoogle Scholar
  59. 59.
    Yun, A.J., and Doux, J.D. (2007) Unhappy meal: how our need to detect stress may have shaped our preferences for taste. Medical Hypotheses69, 746–751PubMedCrossRefGoogle Scholar
  60. 60.
    Schultz, J.C. (2002) Shared signals and the potential for phylogenetic espionage between plants and animals. Int Comp Biology42, 454–462CrossRefGoogle Scholar
  61. 61.
    Alpi, A. et al (2007) Plant neurobiology: no brain, no gain? Trends Plant Sci12, 135–136PubMedCrossRefGoogle Scholar
  62. 62.
    Brenner, E.D. et al (2006) Plant neurobiology: an integrated view of plant signaling. Trends Plant Sci11, 413–419PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Plant Cellular and Molecular BiologyThe Ohio State UniversityColumbusUSA

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