Immunohistochemistry Applications

  • Basil S. Cherpelis
  • L. Frank Glass
  • John R. HamillJr
  • Neil A. Fenske


Immunohistochemistry can be applied judiciously in the delineation of tumoral histiogenesis and the extent of lesional involvement in frozen section pathology. Among the more important immunostains and applications are the use of the MART-1 stain in melanoma, cytokeratin immuno stain in cutaneous epithelial malignancy and Ber-EP4 in basal cell carcinoma.


Freeze Section Hair Follicle Basal Cell Carcinoma Merkel Cell Carcinoma Sebaceous Carcinoma 
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  1. 1.
    Bricca GM, Brodland DG, Zitelli JA. Immunostaining melanoma frozen sections: the 1-hour protocol. Dermatol Surg. 2004;30:403–408.CrossRefPubMedGoogle Scholar
  2. 2.
    Cherpelis BS, Logan T, Ladd S. 19 Minute Rapid Cytokeratin Immunostaining of Nonmelanoma Skin Cancer in Mohs Micrographic Surgery. Dermatol Surg. Accepted for publication.Google Scholar
  3. 3.
    Cherpelis BS, Moore R, Ladd S, et al. Comparison of MART-1 Frozen Sections to Permanent Sections Using A Rapid 19 Minute Protocol. Dermatol Surg. Accepted for publication.Google Scholar
  4. 4.
    Mondragon RM, Barrett TL. Current Concepts: the use of immunoperoxidase techniques in Mohs micrographic surgery. J Am Acad Dermatol. 2000;43(1):66–71.CrossRefPubMedGoogle Scholar
  5. 5.
    Rapini RP. Pitfalls of Mohs micrographic surgery. J Am Acad Dermatol. 1990;22:681–686.CrossRefPubMedGoogle Scholar
  6. 6.
    Smeets NW, Stavats-Kooy AJ, Krekels GA, et al. Adjuvant cytokeratin staining in Mohs micrographic surgery for basal cell carcinoma. Dermatol Surg. 2003;29:375–377.CrossRefPubMedGoogle Scholar
  7. 7.
    Tellechea O, Reis JP, Domingues JC, Baptista AP. Monoclonal antibody Ber EP4 distinguises basal-cell carcinoma from squamous-cell carcinoma of the skin. Am J Dermatopathol. 1993;15(5):452–455.CrossRefPubMedGoogle Scholar
  8. 8.
    Zachary CB, Rest EB, Furlong SM, Arcedo PN, et al. Rapid cytokeratin stains enhance the sensitivity of Mohs micrographic surgery for squamous cell carcinoma. J Dermatol Surg Oncol. 1994;20:530–535.PubMedGoogle Scholar
  9. 9.
    Zalla MJ, Lim KK, Dicuado DJ, Gagnot MM. Mohs micrographic excision of melanoma using immunostains. Dermatol Surg. 2000;26:771–784.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Basil S. Cherpelis
    • 1
  • L. Frank Glass
    • 2
  • John R. HamillJr
    • 3
    • 4
  • Neil A. Fenske
    • 5
  1. 1.Department of DermatologyUniversity of South Florida College of MedicineTampaUSA
  2. 2.Department of Dermatology, Pathology, and Cutaneous Oncology, H. Lee Moffitt Cancer Center and Research InstituteUniversity of South Florida College of MedicineTampaUSA
  3. 3.Gulf Coast DermatologyTampaUSA
  4. 4.University of South Florida, and Director, Advance Dermatological Surgery, James A. Haley Veterans HospitalTampaUSA
  5. 5.Department of Dermatology and Cutaneous SurgerUniversity of South Florida College of MedicineTampaUSA

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