Petunia pp 71-84 | Cite as

ADH and PDC: Key Roles for Enzymes of Alcoholic Fermentation

  • Judith Strommer
  • Freydoun Garabagi


In plants the enzymes pyruvate decarboxylase and alcohol dehydrogenase are generally associated with the alcoholic fermentation pathway, producing a diffusible, non-acidic, and relatively non-toxic end-product for anaerobic glycolysis while regenerating a small amount of NAD+ and ATP. Work with Petunia and tobacco has provided evidence that a more critical function in pollen, and probably other organs and tissues, is to feed carbon back into general metabolism by way of a pyruvate dehydrogenase (PDH) bypass. Alcohol dehydrogenase is also linked to the biosynthetic pathway producing linear six-carbon volatiles, and perhaps some aromatic volatiles, associated with attraction of insect pollinators.


Green Fluorescent Protein Pollen Tube Alcoholic Fermentation Anaerobic Fermentation Floral Scent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Armstrong, W. and Beckett, P.M. (1987) Internal aeration and the development of stelar anoxia in submerged roots: A multishelled mathematical model combining axial diffusion of O in the cortex with radial losses to the stele, the wall layers and the rhizosphere. New Phytol. 105, 221–245.CrossRefGoogle Scholar
  2. Berger, J. and Avery, G.S., Jr. (1943) Dehydrogenases of the Avena coleoptile. Amer. J. Bot. 30, 290–297.CrossRefGoogle Scholar
  3. Biale, J.B. (1946) Effect of oxygen concentration on respiration of the Fuerte avocado fruit. Amer. J. Bot. 33, 363–373.CrossRefGoogle Scholar
  4. Bucher, M., Brander, K.A., Sbicego, S., Mandel, T. and Kuhlemeier, C. (1995) Aerobic fermentation in tobacco pollen. Plant Molec. Biol. 28, 739–750.CrossRefGoogle Scholar
  5. Christie, P.J., Hahn, M. and Walbot, V. (1991) Low-temperature accumulation of alcohol dehydrogenase-1 mRNA and protein activity in maize and rice seedlings. Plant Physiol. 95, 699–706.CrossRefPubMedGoogle Scholar
  6. Desikan, R., Machkerness, S.A.H., Hancock, J.T. and Neill, S.J. (2001) Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol. 127, 159–172.CrossRefPubMedGoogle Scholar
  7. Dolferus, R., Jacobs, M., Peacock, W.J. and Dennis, E.S. (1994) Differential interactions of promoter elements in stress responses of the Arabidopsis Adh gene. Plant Physiol. 105, 1075–1087.CrossRefPubMedGoogle Scholar
  8. Drew, M.C. (1990) Sensing soil oxygen. Plant Cell Environ. 13, 1013–1023.CrossRefGoogle Scholar
  9. Dudareva, N. and Pichersky, E. (2000) Biochemical and molecular genetic aspects of floral scents. Plant Physiol. 122, 627–633.CrossRefPubMedGoogle Scholar
  10. Fahn, A. (1979) Secretory Tissues in Plants. Academic Press, London.Google Scholar
  11. Foster Atkinson, E. (1996) Alcohol dehydrogenase gene expression in petunia, Ph.D. Dissertation, University of Guelph.Google Scholar
  12. Foster Atkinson, E., Cameron, L.A. and Strommer, J.N. (1996) Isolation and characterization of the Adh2 5ʹ region from Petunia hybrida. Plant Molec. Biol. 30, 367–371.CrossRefGoogle Scholar
  13. Garabagi, F. and Strommer, J. (2004) Distinct genes produce the alcohol dehyderogenases of pollen and maternal tissues in Petunia hybrida. Biochem. Gen. 42, 199–208.CrossRefGoogle Scholar
  14. Garabagi, F., Duns, G. and Strommer, J. (2005) Selective recruitment of Adh genes for distinct enzymatic functions in Petunia hybrida. Plant Molec. Biol. 58, 283–294.CrossRefGoogle Scholar
  15. Gass, N., Glagotskaia, T., Mellema, S., Stuurman, J., Barone, M., Mandel, T., Roessner-Tunali, U. and Kuhlemeier, C. (2005) Pyruvate decarboxylase provides growing pollen tubes with a competitive advantage in petunia. Plant Cell 17, 2355–2368.CrossRefPubMedGoogle Scholar
  16. Gregerson, R., McLean, M., Beld, M., Gerats, A.G.M. and Strommer, J. (1991) Structure, expression, chromosomal location and product of the gene encoding ADH1 in Petunia. Plant Molec. Biol. 17, 37–48.CrossRefGoogle Scholar
  17. Gregerson, R.G., Cameron, L., McLean, M., Dennis, P. and Strommer, J. (1993) Structure, expression, chromosomal location and product of the gene encoding ADH2 in Petunia. Genet. 133, 999–1007.Google Scholar
  18. Hageman, R.H. and Flesher, D. (1960) The effect of an anaerobic environment on the activity of alcohol dehydrogenase and other enzymes of corn seedlings. Arch. Biochem. Biophys. 87, 203–209.CrossRefPubMedGoogle Scholar
  19. Hatanaka, A. (1993) The biogeneration of green odour by green leaves. Phytochem. 34, 1201–1218.CrossRefGoogle Scholar
  20. Hoballah, M.E., Stuurman, J., Turlings, T.C.J., Guerin, P.M., Connétble, S. and Kuhlemeier, C. (2005) The composition and timing of flower odour emission by wild Petunia axillaris coincide with the antennal perception and nocturnal activity of the pollinator Manduca sexta. Planta 222, 141–150.CrossRefPubMedGoogle Scholar
  21. Hoffman, N.E., Bent, A.F., Hanson, A.D. (1986) Induction of lactate dehydrogenase isozymes by oxygen deficit in barley root tissue. Plant Physiol. 82, 658–663.CrossRefPubMedGoogle Scholar
  22. Johnson, J.R., Cobb, J.B. and Drew, M.C. (1994) Hypoxic induction of anoxia tolerance in roots of Adh1 null Zea mays L. Plant Physiol. 105. 61–67.Google Scholar
  23. Kato-Noguchi, H. (2001) Wounding stress induces alcohol dehydrogenase in maize and lettuce seedlings. Plant Growth Regul. 35, 285–288.CrossRefGoogle Scholar
  24. Kimmerer, T.W. and Kozlowski, T.T. (1982) Ethylene, ethane, acetaldehyde and ethanol production under stress. Plant Physiol. 69, 840–847.CrossRefPubMedGoogle Scholar
  25. Kloeckener-Gruissem, B. and Freeling, M. (1987) Relationship between anaerobic inducibiity and tissue-specific expression for the maize anaerobic genes. In: D. Von Wettstein and N.-H. Chua (Eds.), Plant Molecular Biology, Plenum Press, NY, pp. 293–303.Google Scholar
  26. Laszlo, A. and St. Lawrence, P. (1983) Parallel induction and synthesis of PDC and ADH in anoxic maize roots. Mol. Gen. Genet. 192, 110–117.CrossRefGoogle Scholar
  27. Linskens, H.F. and Schrauwen, J. (1966) Measurement of oxygen tension changes in the style during pollen tube growth. Planta 71, 98–106.CrossRefGoogle Scholar
  28. Mellema, S., Eichenberger, W., Rawyler, A., Suter, M., Tadege, M. and Kuhlemeier, C. (2002) The ethanolic fermentation pathway supports respiration and lipid biosynthesis in tobacco pollen. Plant J. 30, 329–336.CrossRefPubMedGoogle Scholar
  29. Mitchell, W.C. and Jelenkovic, G. (1995) Characterizing NAD- and NADP-dependent alcohol dehydrogenase enzymes of strawberries. J. Amer. Soc. Hort. Sci. 120, 798–801.Google Scholar
  30. Roberts, J.K., Callis, J., Wemmer, D., Walbot, V. and Jardetzky, O. (1984) Mechanism of cytoplasmic pH regulation in hypoxic maize root tips and its role in survival under hypoxia. Proc. Natl. Acad. Sci., USA 81, 3379–3383.CrossRefPubMedGoogle Scholar
  31. Roberts, J.K.M., Chang, K., Webster, C., Callis, J. and Walbot, V. (1988) Dependence of ethanolic fermentation, cytoplasmic pH regulation, and viability on the activity of alcohol dehydrogenase in hypoxic maize root tips. Plant Physiol. 89, 1275–1278.CrossRefGoogle Scholar
  32. Schwartz, D. (1966) The genetic control of alcohol dehydrogenase in maize: Gene duplication and repression. Proc. Natl. Acad. Sci., USA 56, 1431–1436.CrossRefPubMedGoogle Scholar
  33. Schwartz, D. and Endo, T. (1966) Alcohol dehydrogenase polymorphism in maize – simple and compound loci. Genet. 53, 709–715.Google Scholar
  34. Schwartz, D. (1969) An example of gene fixation resulting from selective advantage in sub-optimal conditions. Amer. Nat. 103, 479–481.CrossRefGoogle Scholar
  35. Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M., Enju, A., Sakurai, T. et al. (2002) Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. Plant J. 31, 279–292.CrossRefPubMedGoogle Scholar
  36. Sink, K.C. (1984) Chapter 3: Anatomy and morphology. In: K.C. Sink (Ed.), Petunia: Monographs on Theoretical and Applied Genetics 9. Springer Verlag, Berlin, pp. 10–20.Google Scholar
  37. Speirs, J., Lee, E., Holt, K., Yong-Duk, K., Scott, N.S., Loveys, B. and Schuch, W. (1998) Genetic manipulation of alcohol dehydrogenase levels in ripening tomato fruit affects the balance of some flavor aldehydes and alcohols. Plant Physiol. 117, 1047–1058.CrossRefPubMedGoogle Scholar
  38. Strommer, J., Gregerson, R.G., Foster, E. and Huang, S. (1993) Alcohol dehydrogenase (Adh) genes and their expression in higher plants: Maize and petunia. In: P.M. Gresshoff (Ed.), Plant Responses to the Environment, CRC Press, Boca Raton FL, pp. 11–24.Google Scholar
  39. Strommer, J., Gerats, A.G.M., Sanago, M. and Molnar, S.J. (2000) A gene-based RFLP map of Petunia. Theor. Appl. Gen. 100, 899–905.CrossRefGoogle Scholar
  40. Strommer, J., Gerats, A.G.M., Sanago, M. and Molnar, S.J. (2001) Erratum: A gene-based RFLP map of Petunia. Theor. Appl. Gen. 102, 1305–1306.CrossRefGoogle Scholar
  41. Tadege, M. and Kuhlemeier, C. (1997) Aerobic fermentation during tobacco pollen development. Plant Molec. Biol. 35, 343–354.CrossRefGoogle Scholar
  42. Tadege, M., Dupuis, I. and Kuhlemeier, C. (1999) Ethanolic fermentation: New functions for an old pathway. Trends Plant Sci. 4, 320–325.CrossRefPubMedGoogle Scholar
  43. Thomson, C.J. and Greenway, H. (1991) Metabolic evidence for stellar anoxia in maize roots exposed to low O2 concentrations. Plant Physiol. 96, 1294–1301.CrossRefPubMedGoogle Scholar
  44. Van Eldik, G.J., Ruiter, R.K., van Herpen, M.M.A., Schrauwen, J.A.M. and Wullems, G.J. (1997) Induced ADH gene expression and enzyme activity in pollinated pistils of Solanum tuberosum. Sex. Plant Reprod. 10, 107–109.CrossRefGoogle Scholar
  45. Zhang, M., Maeda, Y., Furihata, F., Nakamaru, Y. and Esashi, Y. (1994) A mechanism of seed deterioration in relation to the volatile compounds evolved by dry seeds themselves. Seed Sci. Res. 4, 49–56.CrossRefGoogle Scholar
  46. Zhang, M., Nakamaru, Y., Tsuda, S., Nagashima, T. and Esashi, Y. (1995a) Enzymatic conversion of volatile metabolites in dry seeds during storage. Plant Cell Physiol. 36, 157–164.Google Scholar
  47. Zhang, M., Yoshiyama, M., Nagashima, T., Nakagawa, Y., Yoshioka, T. and Esashi, Y. (1995b) Aging of soybean seeds in relation to metabolism at different relative humidities. Plant Cell Physiol. 36, 1189–1195.Google Scholar
  48. Zhang, M., Nagata, S., Miyazawa, K., Kikuchi, H. and Esashi, Y. (1997) A competitive enzyme-linked immunosorbent assay to quantify acetaldehyde-protein adducts that accumulate in dry seeds during aging. Plant Physiol. 113, 397–402.CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Judith Strommer
    • 1
  • Freydoun Garabagi
  1. 1.Department of Plant Agriculture, Bovey BuildingUniversity of GuelphGuelphCanada

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