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Synaptoproteomics of Existing and new Animal Models of Depression

  • Alessandra Mallei
  • Roberto Giambelli
  • Aram El Khoury
  • Susanne H. M. Gruber
  • Laura Musazzi
  • Valentina S. Barbiero
  • Daniela Tardito
  • Barbara Vollmayr
  • Peter Gass
  • Aleksander A. Mathé
  • Giorgio Racagni
  • Maurizio Popoli
Chapter

Abstract

Depression is a severe and life-threatening psychiatric illness whose pathogenesis is still essentially unknown. Proteomic analysis of synaptic terminals (synaptoproteomics) in animal models of depression is a powerful approach to gain insight into the molecular mechanisms underlying vulnerability to mood disorders and the long-term action of drug treatments. Here, we employed two different animal models of depression, the Learned Helplessness rats (a classical behavioral model of depression) and a new model of depression with gene—environment interaction (Flinders Sensitive Line rats subjected to early life stress). Both animal models were treated with the antidepressant escitalopram. Analysis of their synaptoproteomic profile revealed a number of protein spots differently regulated by basic vulnerability and/or early life stress. Using this approach, we obtained information regarding biomarkers that may represent predictors of pathology or response/resistance to drug treatment, as well as potential targets for novel pharmacological and therapeutic strategies.

Keywords

Protein Spot Antidepressant Treatment Maternal Separation Early Life Stress Flinder Sensitive Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

2D

Two-dimensional

5-HT1A

Serotonin 1A receptor

BCA

Bicinchoninic Acid

CHAPS

(3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate)

DA

Dopamine

DAVID

Database for Annotation, Visualization and Integrated Discovery

ESC

Escitalopram

FRL

Flinders sensitive line

FSL

Flinders resistant line

G × E

Gene—environment interaction

GO

Gene Ontology

HC

Hippocampus

HPA

Hypothalamic—pituitary—adrenal

IBS

Inflammatory bowel syndrome

IEF

Isoelectric focusing

IPA

Ingenuity pathways analysis

IPG

Immobilized pH gradient

LH

Learned helplessness

mRNA

Messenger RNA

MS

Maternal separation

nLH

Non-learned helplessness

NMDA

N-methyl-D-aspartate

P/FC

Prefrontal/frontal cortex

pI

Isoelectric point

PND

Post natal day

REM

Random eyes movement

SDS

Sodium dodecyl sulfate

SDS-PAGE

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

Tris

Tris(hydroxymethyl)aminomethane

Veh

Vehicle

Notes

Acknowledgments

This work was funded by a European Union (6th Framework Program) grant for project GENDEP (contract no. LSHB-CT-2003-503428) and by the Swedish Medical Research Council grant 10414 (to AAM).

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Alessandra Mallei
  • Roberto Giambelli
  • Aram El Khoury
  • Susanne H. M. Gruber
  • Laura Musazzi
  • Valentina S. Barbiero
  • Daniela Tardito
  • Barbara Vollmayr
  • Peter Gass
  • Aleksander A. Mathé
  • Giorgio Racagni
  • Maurizio Popoli
    • 1
  1. 1.Center of Neuropharmacology, Department of Pharmacological SciencesUniversity of MilanoMilanoItaly

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