Abstract
This chapter describes a pilot study aimed at combining protein microarrays with live basophil cells (live basophil allergen array). The aim is to combine the power of microarrays with the specificity and sensitivity of basophil tests, adding a biological dimension to the diagnosis of allergy. Allergens or control proteins were spotted onto FAST slides and incubated with purified peripheral blood basophils for different lengths of time. In the initial set of experiments, binding of basophils to the microarray was obtained by spotting anti-human IgE antibodies onto the array, and visualized by conventional light microscopy. Further proof-of-concept experiments used purified peripheral blood basophils that had been IgE-stripped by a short lactic acid buffer incubation and resensitized with an atopic donor’s serum with known IgE specificity, in combination with the relevant pollen allergens. Activation of basophils was measured by detecting CD63, as an indirect measurement of basophil degranulation. The potential pitfalls of the live basophil allergen array are discussed in the context of basophil immunobiology.
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References
Barinaga, M.W. (1991) “DNA chip” speed genome initiative. Science 253, 1489.
Belov, L., de la Vega, O., dos Remedios, C.G., Mulligan, S.P. and Christopherson, R.I. (2001). Immunophenotyping of leukemias using a cluster of differentiation antibody microarray. Cancer Res. 61, 4483–4489.
Blackley C. (1873) Experimental Researches on the Cause and Nature of Catarrhus Aestivus, (London).
Blackley, C.H. (1880) Hay Fever; its Causes, Treatment, and Effective Prevention. Ballière, (London).
Bühring, H.J., Streble, A. and Valent, P. (2004) The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis. Int Arch Allergy Immunol 133(4), 317–329.
Bühring, H.J., Seiffert, M., Giesert, C., Marxer, A., Kanz, L., Valent, P. and Sano, K. (2001) The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/-phosphodieste-rase 3. Blood 97, 3303–3305.
Burastero, S.E., Paolucci, C., Breda, D., Monasterolo, G., Rossi, R.E., and. Vangelista L. (2006) Unreliable Measurement of Basophil Maximum Leukotriene Release with the Bühlmann CAST 2000 Enzyme-Linked Immunosorbent Assay Kit. Clin Vaccine Immunol 13(3), 420–422.
Cambier, J.C. (1995) Antigen and Fc receptor signaling. The awesome power of the immunoreceptor tyrosine-based activation motif (ITAM). J Immunol 155, 3281–3285.
Demoly, P., Lebel, B. and Arnoux, B. (2003) Allergen-induced mediator release tests. Allergy 58, 553–558.
Eiseman, E., and Bolen. J.B. (1992) Nature 355, 78–80.
Foetisch, K., Westphal, S., Lauer, I., Retzek M., Altmann, F., Kolarich, D., Scheurer, S. and Vieths, S. (2003) Biological activity of IgE specific for cross-reactive carbohydrate determinants. J Allergy Clin Immunol 111, 889–896.
Gibbs, B.F., Räthling, A., Zillikens, D., Huber, M. and Haas, H. (2006) Initial FcɛRI-mediated signal strength plays a key role in regulating basophil signaling and deactivation. J Allergy Clin Immunol 118, 1060–1067.
Golden, D.B., Tracy, J.M., Freeman, T.M. and Hoffman, D.R. (2003) Negative venom skin test results in patients with histories of systemic reaction to a sting. J Allergy Clin Immunol 112, 495–498.
Haas, H., Falcone, F.H., Schramm, G., Haisch, K., Gibbs, B.F., Klaucke, J., Poppelmann, M., Becker, W.M., Gabius, H.J. and Schlaak. M. (1999) Dietary lectins can induce in vitro release of IL-4 and IL-13 from human basophils. Eur J Immunol 29, 918–97.
Harwanegg, C., Laffer, S., Hiller, R., Mueller, M.W., Kraft, D., Spitzauer, S. and Valenta, R. (2003). Microarrayed recombinant allergens for diagnosis of allergy. Clin Exp Allergy 33, 7–13.
Hennersdorf, F., Florian, S., Jakob, A., Baumgartner, K., Sonneck, K., Nordheim, A., Biedermann, T., Valent, P. and Bühring, H.J. (2005) Identification of CD13, CD107a, and CD164 as novel basophil-activation markers and dissection of two response patterns in time kinetics of IgE-dependent upregulation. Cell Res 15, 325–335.
Hiller R., Laffer S., Harwanegg C., Huber M., Schmidt W.M., Twardosz A., Barletta B., Becker W.M., Blaser K., Breiteneder H., Chapman M., Crameri R., Duchene M., Ferreira F., Fiebig H., Hoffmann-Sommergruber K., King T.P., Kleber-Janke T., Kurup V.P., Lehrer S.B., Lidholm J., Muller U., Pini C., Reese G., Scheiner O., Scheynius A., Shen H.D., Spitzauer S., Suck R., Swoboda I., Thomas W., Tinghino R., Van Hage-Hamsten M., Virtanen T., Kraft D., Muller M.W., Valenta R. Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment. FASEB J 2002 Mar; 16(3), 414–6.
Holowka, D. and Baird, B. (2001) FcɛRI as a paradigm for a lipid raft-dependent receptor in hematopoietic cells. Semin Immunol. 13, 99–105.
Jouvin, M.H., Numerof, R.P., Kinet, J.P. (1995) Signal transduction through the conserved motifs of the high affinity IgE receptor Fc epsilon RI. Semin Immunol 7, 29–35.
Kepley, C.L., Youssef, L., Andrews, R.P., Wilson, B.S. and Oliver, J.M. (1994) Syk deficiency in nonreleaser basophils. J Allergy Clin Immunol 104, 279–284.
Kepley, C.L., Youssef, L., Andrews, R.P., Wilson, B.S. and Oliver, J.M. (2000) Multiple defects in Fc epsilon RI signaling in Syk-deficient nonreleaser basophils and IL-3-induced recovery of Syk expression and secretion. J Immunol 165, 5913–5920.
Kleine-Tebbe, J., Erdmann, S., Knol, E.F., MacGlashan, D.W., Poulsen, L.K. and Gibbs, B.F. (2006) Diagnostic tests based on human basophils: potential, pitfalls and perspectives. Int Arch Allergy Immunol 141, 79–90.
Knol, E. F., Mul, F. P., Jansen, H., Calafat, J. and Roos, D. (1991) Monitoring human basophil activation via CD63 monoclonal antibody 435. J Allergy Clin Immunol 88, 328–338.
Knol, E.F., Kuijpers, T.W., Mul, F.P. and Roos, D. (1993) Stimulation of human basophils results in homotypic aggregation. A response independent of degranulation. J Immunol 151, 4926–4933.
Lin, J., Renault, N., Haas, H., Schramm, G., Vieths, S., Vogel, L., Falcone, F.H. and Alcocer, M.J. (2007) A novel tool for the detection of allergic sensitization combining protein microarrays with human basophils. Clin Exp Allergy. 37, 1854–1862.
Lin, J., Shewry, P.R., Archer, D.B., Beyer, K., Niggemann, B., Haas, H., Wilson, P., Alcocer, M.J. (2006). “The Potential Allergenicity of Two 2S Albumins from Soybean (Glycine max): A Protein Microarray Approach.” Int Arch Allergy Immunol 141(2), 91–102.
Lueking, A., Horn, M., Eickhoff, H., Bussow, K., Lehrach, H. and Walter, G. (1999) Protein Microarrays for gene expression and antibody screening. Anal Biochem 270, 103–111.
Mahmudi-Azer, S., Downey, G.P. and Moqbel, R. (2002) Translocation of the tetraspanin CD63 in association with human eosinophil mediator release. Blood, 99 (11), 4039–4047.
Mari, A., Iacovacci, P., Afferni, C., Barletta, B., Tinghino, R., Di Felice, G. and Pini, C. (1999) Specific IgE to cross-reactive carbohydrate determinants strongly affect the in vitro diagnosis of allergic diseases. J Allergy Clin Immunol 103, 1005–1.
McDonnell J.M., Calvert, R., Beavil, R.L., Beavil, A.J., Henry, A.J., Sutton, B.J., Gould, H.J. and Cowburn (2001) The structure of the IgE Cɛ2 domain and its role in stabilizing the complex with its high-affinity receptor FcɛRIα. Nat Struct Biol 8, 437–441.
Medrala, W., Malolepszy, J., Medrala, A.W., Liebhart, J., Marszalska, M., Dobek, R., Gietkiewicz, K., Litwa, M. and Pietrzak, E. (1997) CAST-ELISA test-a new diagnostic tool in pollen allergy. J Investig Allergol Clin Immunol 7, 32–35.
Mousses, S., Caplen, N.J., Cornelison, R., Weaver, D., Basik, M., Hautaniemi, S., Elkahloun, A.G., Lotufo, R.A., Choudary, A., Dougherty, E.R., Suh, E. and Kallioniemi O. (2003) RNAi microarray analysis in cultured mammalian cells. Genome Res 13(10), 2341–2347.
Nguyen, K.L., Gillis, S. and MacGlashan, D.W. Jr. (1990) A comparative study of releasing and nonreleasing human basophils: nonreleasing basophils lack an early component of the signal transduction pathway that follows IgE cross-linking. J Allergy Clin Immunol 85, 1020–1029.
Novak, N., Kraft, S. and Bieber, T. (2001) IgE receptors. Curr Opin Immunol 13, 721–726.
Palmer, E.L., Miller, A.D. and Freeman, T.C. (2006) Identification and characterisation of human apoptosis inducing proteins using cell-based transfection microarrays and expression analysis. BMC Genomics 7, 145.
Pierini, L., Holowka, D. and Baird, B. (1996). FcɛRI-mediated association of 6-μm beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling. J Cell Biol 134, 1427–1439.
Pirquet, C. (1906) Klinische Studien über Vakzination und vakzinale Allergie. Münchener medizinische Wochenschrift, 53, 1457–1458.
Pruzansky, J.J. and Patterson, R. (1986) Binding constants of IgE receptors on human blood basophils for IgE. Immunology, 58, 257–262.
Ravetch, J.V. and Kinet, J.P. (1991) Fc receptors. Annu Rev Immunol 9, 457–492.
Senna, G., Bonadonna, P., Crivellaro, M., Schiappoli, M. and Passalacqua, G. (2005) Anaphylaxis due to Brazil nut skin testing in a walnut-allergic subject. J Investig Allergol Clin Immunol 5, 225–227.
Vonakis, B.M., Haleem-Smith, H., Benjamin, P. and Metzger. H. (2001) Interaction between the unphosphorylated receptor with high affinity for IgE and Lyn kinase. J Biol Chem 276, 1041–1050.
Wide, L., Bennich, H. and Johansson, S.G. (1967) Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet 2 (7526), 1105–1179.
Ziauddin, J. and Sabatini, D.M. (2001) Microarrays of cells expressing defined cDNAs. Nature 411(6833), 107–110.
Acknowledgments
Helmut Haas and Bernhard F. Gibbs have been supported in part by a grant from the Deutsche Forschungsgemeinschaft (SFB/TRR 22). Franco Falcone is supported by the Wellcome Trust (Grant no. GR065978MA). Lin Jing and Marcos Alcocer are supported in part by a University of Nottingham’s New Lecturers Fund.
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Falcone, F.H. et al. (2009). The Live Basophil Allergen Array (LBAA): A Pilot Study. In: Falus, A. (eds) Clinical Applications of Immunomics. Immunomics Reviews, vol 2. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79208-8_8
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