Abstract
Genetic gain from phenotypic selection in open-pollinated forage species is constrained by the inability to accurately use phenotype to estimate genotype, prior to parent selection for polycrossing. The use of marker-assisted selection (MAS) offers the potential to accelerate genetic gain by partially overcoming this constraint. White clover (Trifolium repens L., 2n = 4x = 32) is an open-pollinated, high-quality, perennial forage legume with complex inheritance of traits underpinning pasture persistence, seed production, animal productivity, and animal health. Our legume improvement programme has utilised seed production in white clover as a case study in the application of MAS in outbred forage species, using microsatellite markers linked to quantitative trait loci (QTL) of moderate resolution. The QTL SY03-D2 on the distal end of group D2, was used to explore marker:trait associations in 12 breeding pools, leading to opportunities to conduct reselection experiments, and to monitor response to genotypic selection criteria in experimental polycrosses. Each breeding pool was sampled with 90 or more individuals grown out in an unreplicated field trial to assess seed yield traits, as per standard practice in our cultivar development programme. DNA samples were tested with up to three microsatellite markers associated with the QTL. Significant (p < 0.01) marker:trait associations were observed in 8 of the 12 breeding pools, with the most informative polymorphisms accounting for differences of 30–69% in the mean seed yield values within breeding pools. These data suggest that value can be realised from the current investment in genomics for MAS in white clover, given QTL of moderate resolution, and widely used marker platforms such as microsatellites.
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References
Barrett B, Griffiths A, Schreiber M, Ellison N, Mercer C, Bouton JH, Ong B, Forster J, Sawbridge T, Spangenburg G, Bryan GJ, Woodfield DR (2004) A microsatellite map of white clover. Theor Appl Genet 109:596–608
Barrett B, Mercer C, Woodfield DR (2005a) Genetic mapping of a root-knot nematode resistance locus in Trifolium. Euphytica 143:85–92
Barrett BA, Baird IJ, Woodfield DR (2005b) A QTL analysis of white clover seed production. Crop Sci 45:1844–1850
Carlborg O, Haley CS (2004) Epistasis: too often neglected in complex trait studies? Nat Rev Genet 5:618–625
Chapman DF, Mackay AD, Devantier BP, Dymock N (1993) Impact of white clover cultivars on nitrogen fixation and livestock production in New Zealand hill pasture. Proc Int Grassl Cong 17:420–421
Cogan NOI, Abberton MT, Smith KF, Kearney G, Marshall AH, Williams A, Michaelson-Yeates TPT, Bowen C, Jones ES, Vecchies AC, Forster JW (2006a) Individual and multi-environment combined analyses identify QTLs for morphogenetic and reproductive development traits in white clover (Trifolium repens. L.) Theor Appl Genet 112:1401–1415
Cogan NOI, Ponting RC, Vecchies AC, Drayton MC, George J, Dracatos PM, Dobrowolski MP, Sawbridge TI, Smith KF, Spangenberg GC, Forster JW (2006b) Gene-associated single nucleotide polymorphism discovery in perennial ryegrass (Lolium perenne. L.) Mol Genet Genomics 276:101–112
Cogan NOI, Drayton MC, Ponting RC, Vecchies AC, Bannan NR, Sawbridge TI, Smith KF, Spangenberg GC, Forster JW (2007) Validation of in silico-predicted genic SNPs in white clover (Trifolium repens. L.), an outbreeding allopolyploid species. Mol Genet Genomics 277:413–425
Crush JR, Woodward SL, Eerens JPJ, MacDonald KA (2006) Growth and milksolids production in pastures of older and more recent ryegrass and white clover cultivars under dairy grazing. N Z J Agric Res 49:119–135
Easton HS, Baird DB, Cameron NE, Kerr GA, Norriss M, Stewart AV (2001) Perennial ryegrass cultivars: herbage yield in multi-site plot trials. Proc NZ Grassl Assoc 63:183–188
Faville M, Vecchies A, Schreiber M, Drayton M, Hughes L, Jones E, Guthridge K, Smith K, Sawbridge T, Spangenberg G, Bryan G, Forster J (2004) Functionally associated molecular genetic marker map construction in perennial ryegrass (Lolium perenne. L.). Theor Appl Genet 110:12–32
Francia E, Tacconi G, Crosatti C, Barabaschi D, Bulgarelli D, Dall'Aglio E, Vale G (2005) Marker assisted selection in crop plants. Plant Cell Tissue Organ Cult 82:317–342
Hayes B, He J, Moen T, Bennewitz J (2006) Use of molecular markers to maximise diversity of founder populations for aquaculture breeding programs. Aquaculture 255:573–578
Helentjaris T, King G, Slocum M, Siedenstrang C, Wegman S (1985) Restriction fragment length polymorphisms as probes for plant diversity and their development as tools for applied plant breeding. Plant Mol Biol 5:109–118
Humphreys MW, Yadav RS, Cairns AJ, Turner LB, Humphreys J, Skøt L (2006) A changing climate for grassland research. New Phytol 169:9–26
Jones ES, Dupal MP, Dumsday JL, Hughes LJ, Forster J (2002) An SSR-based genetic linkage map for perennial ryegrass (Lolium perenne. L.). Theor Appl Genet 105:577–584
Kölliker R, Boller B, Widmer F (2005) Marker assisted polycross breeding to increase diversity and yield in perennial ryegrass (Lolium perenne. L.). Euphytica 146:55–65
Newton PCD, Edwards GR (2006) Plant breeding for a changing environment. In: Newton PCD, Carran RA, Edwards GR, Niklaus PA (eds) Agroecosystems in a changing climate. CRC Press, London, pp 309–322
Powell RL, Norman HD (2006) Major advances in genetic evaluation techniques. J Dairy Sci 89:1337–1348
Sawbridge T, Ong E, Binnion C, Emmerling M, Meath K, Nunan K, O'Neill M, O'Toole F, Simmounds JK, Winkworth A, Spangenburg G (2003) Generation and analysis of expressed sequence tags in white clover (Trifolium repens. L.). Plant Sci 165:1077–1087
Sonesson AK (2007) Within-family marker-assisted selection for aquaculture species. Genet Select Evol 39:301–317
Stendal C, Casler MD, Jung G (2006) Marker-assisted selection for neutral detergent fiber in smooth bromegrass. Crop Sci 46:303–311
Tamaki H, Yoshizawa A, Fujii H, Sato K (2007) Modified synthetic varieties: a breeding method for forage crops to exploit specific combining ability. Plant Breed 126:95–100
Widdup K, Woodfield DR, Baird I, Clifford P (2004) Response to selection for seed yield in six white clover cultivars. Proc N Z Grassl Assoc 66:103–110
Woodfield DR (1999) Genetic improvements in New Zealand forage cultivars. Proc NZ Grassl Assoc 61:3–7
Woodfield DR, Easton HS (2004) Advances in pasture plant breeding for animal productivity and health. N Z Vet J 52:300–310
Woodfield DR, Baird I, Clifford P (2004) Genetic improvement of white clover seed production. Proc NZ Grassl Assoc 66:111–117
Xiong YW, Fei SZ, Brummer EC, Moore KJ, Barker RE, Jung GW, Curley J, Warnke SE (2006) QTL analyses of fiber components and crude protein in an annual × perennial ryegrass interspecific hybrid population. Mol Breed 18:327–340
Yamada T, Jones ES, Cogan NOI, Vecchies AC, Nomura T, Hisano H, Shimamoto Y, Smith KF, Hayward MD, Forster JW (2004) QTL analysis of morphological, developmental, and winter hardiness-associated traits in perennial ryegrass. Crop Sci 44:925–935
Zhang Y, Sledge MK, Bouton JH (2007) Genome mapping of white clover (Trifolium repens. L.) and comparative analysis within the Trifolieae using cross-species SSR markers. Theor Appl Genet 114:1367–1378
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Barrett, B., Baird, I., Woodfield, D. (2009). White Clover Seed Yield: A Case Study in Marker-Assisted Selection. In: Molecular Breeding of Forage and Turf. Springer, New York, NY. https://doi.org/10.1007/978-0-387-79144-9_22
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DOI: https://doi.org/10.1007/978-0-387-79144-9_22
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