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QTL Analysis and Gene Expression Studies in White Clover

  • Michael T. Abberton
  • Athole Marshall
  • Rosemary P. Collins
  • Charlotte Jones
  • Matthew Lowe

Abstract

In white clover (Trifolium repens L.) plant persistence, overwintering and grazing tolerance are to a significant extent determined by the presence of a dense network of horizontal stems or stolons. At IGER, we have developed new linkage maps of white clover specifically to facilitate the identification of quantitative trait loci (QTLs) for important components of stolon morphology. The parents of the F1 mapping family were derived following two generations of divergent selection for stolon traits, in particular length and thickness. One parental genotype had thick, sparse stolons and one parent had thin profuse stolons. Two linkage maps were generated for each parental clone: one consisted of 16 linkage groups with 154 markers covering 710 cM and the other consisted 13 linkage groups with 86 markers covering 524 cM. There are 31 shared markers across the two maps. A total of nine QTLs were identified on the maps for the following traits: stolon width, internode length, petiole length, leaf length and width, plant spread and height. Potential QTLs associated with water use efficiency (WUE) have also been identified.

Keywords

Quantitative Trait Locus Adventitious Root Relative Water Content Quantitative Trait Locus Analysis White Clover 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Michael T. Abberton
    • 1
  • Athole Marshall
    • 1
  • Rosemary P. Collins
    • 1
  • Charlotte Jones
    • 1
  • Matthew Lowe
    • 1
  1. 1.Legume Breeding and Genetics TeamInstitute of Grassland and Environmental Research (IGER)AberystwythUK

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