QTL Analysis and Gene Expression Studies in White Clover
In white clover (Trifolium repens L.) plant persistence, overwintering and grazing tolerance are to a significant extent determined by the presence of a dense network of horizontal stems or stolons. At IGER, we have developed new linkage maps of white clover specifically to facilitate the identification of quantitative trait loci (QTLs) for important components of stolon morphology. The parents of the F1 mapping family were derived following two generations of divergent selection for stolon traits, in particular length and thickness. One parental genotype had thick, sparse stolons and one parent had thin profuse stolons. Two linkage maps were generated for each parental clone: one consisted of 16 linkage groups with 154 markers covering 710 cM and the other consisted 13 linkage groups with 86 markers covering 524 cM. There are 31 shared markers across the two maps. A total of nine QTLs were identified on the maps for the following traits: stolon width, internode length, petiole length, leaf length and width, plant spread and height. Potential QTLs associated with water use efficiency (WUE) have also been identified.
KeywordsQuantitative Trait Locus Adventitious Root Relative Water Content Quantitative Trait Locus Analysis White Clover
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- Barrett, B., Griffiths, A., Schreiber, M., Ellison, N., Mercer, C., Bouton, J., Ong, B., Forster, J., Sawbridge, T., Spangenberg, G., Bryan, G., Woodfield, D. (2004) A microsatellite map of white clover. Theoretical and Applied Genetics, 109, 596–608Google Scholar
- Caradus, J.R. (1981) Root growth of white clover (Trifolium repens L.) lines in glass-fronted containers New Zealand Journal of Agricultural Research 24, 43–54Google Scholar
- Cogan, N.O.I, Abberton, M.T, Smith, K.F, Kearney, G, Marshall, A.H, Williams, A, Michaelson-Yeates, T.P.T, Bowen, C, Jones, E.S, Vecchies, A.C, Forster, J.W (2006) Individual and multi-environment combined analyses identify QTLs for morphogenetic and reproductive development traits in white clover (Trifolium repens. L.) Theoretical and Applied Genetics 112, 1401–1415CrossRefGoogle Scholar
- Febrer, M., Jenkins, G., Abberton, M.T. (2005) Towards a comparative map of white clover (Trifolium repens, ) and barrel medic (Medicago truncatula) Molecular Breeding for the Genetic Improvement of Forage Crops and Turf. Aberystwyth, Wales, Humphreys, M.O., eds., Proceedings of the 4th International Symposium on the Molecular Breeding of Forage and Turf. XXth International Grassland Congress July 2005 193, Wageningen Academic PublishersGoogle Scholar
- Zhang, Y., Sledge, M.K., Bouton, J.H. (2007) QTL mapping of morphological traits and agronomic traits in white clover. Proceedings of International Plant and Animal Genomics XV. January 13–17, 2007, San Diego, CA, USAGoogle Scholar