Advertisement

Proanthocyanidin Biosynthesis in Forage Legumes with Especial Reference to the Regulatory Role of R2R3MYB Transcription Factors and Their Analysis in Lotus japonicus

  • Mark P. Robbins
  • David Bryant
  • Samantha Gill
  • Phillip Morris
  • Paul Bailey
  • Tracey Welham
  • Cathie Martin
  • Trevor L. Wang
  • Takakazu Kaneko
  • Shusei Sato
  • Satoshi Tabata
  • Francesco Paolocci

Abstract

Proanthocyanidins (condensed tannins) can play an important part in ruminant nutrition both by increasing ruminal efficiency and preventing pasture bloat. In this chapter we discuss the control of this pathway by transcription factors and focus particularly upon genes of the bHLH and R2R3MYB classes. Results from studies using transgenic approaches, tilling and similar techniques are discussed here.

Keywords

Condensed Tannin Forage Legume Lotus Japonicus bHLH Gene Lotus Corniculatus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aerts RJ, Barry TN, McNabb WC (1999) Polyphenols and agriculture: beneficial effects of proanthocyanidins in forages. Agric Ecosyst Environ 75: 1–12CrossRefGoogle Scholar
  2. Bailey P, Bryant DB, Welham T, Perry JA, Pike JM, Asamizu E, Tabata S, Sato S, Morris P, Robbins MP, Martin C, Wang TL (2007) Mining myb genes in Lotus japonicus. Proceedings of the 3rd International Conference on Legume Genomics and Genetics, Brisbane, Australia, p 709–13 April 2007,Google Scholar
  3. Baudry A, Heim MA, Dubreucq B, Caboche M, Weisshaar B, Lepiniec L (2004) TT2, TT8 and TTG1 synergistically specify the expression of BANYULS and proanthocyanidins biosynthesis in Arabidopsis thaliana. Plant J 39: 366–380CrossRefPubMedGoogle Scholar
  4. Bryant DB, Bailey P, Morris P, Robbins MP, Wang TL, Martin C (2005) Identification of putative AtTT2 R2R3-MYB transcription factor orthologues in tanniferous tissues of L. corniculatus var. japonicus cv Gifu. In: Humphreys MO (ed) Molecular breeding for genetic improvement of forage crops and turf. Wageningen Academic Publishers, The Netherlands, p 166Google Scholar
  5. Carron TR, Robbins MP, Morris P (1994) Genetic modification of condensed tannin biosynthesis in Lotus corniculatus 1. Heterologous antisense dihydroflavonol reductase down-regulates tannin accumulation in ‘hairy root’ cultures. Theor Appl Genet 76: 1006–1015Google Scholar
  6. de Majnik J, Weinman JJ, Djordjevik MA, Rolfe BG, Tanner GJ, Joseph RG, Larkin PJ (2000) Anthocyanin regulatory gene expression in transgenic white clover can result in an altered pattern of pigmentation. Aust J Plant Physiol 27: 659–667Google Scholar
  7. Furukawa T, Maekawa M, Oki T, Suda I, Iida S, Shimada H, Takamure I, Kadowaki K (2007) The Rc and Rd genes are involved in proanthocyanidin synthesis in rice pericarp. Plant J 49: 91–102CrossRefPubMedGoogle Scholar
  8. Jander G, Barth C (2007) Tandem gene arrays: a challenge for functional genomics. Trends Plant Sci 12: 203–210CrossRefPubMedGoogle Scholar
  9. Martin C, Paz-Ares J (1997) MYB transcription factors in plants. TIGS 13: 67–73Google Scholar
  10. Paolocci F, Bovone T, Tosti N, Arcioni S, Damiani F (2005) Light and an exogenous transcription factor qualitatively and quantitatively affect the biosynthetic pathway of condensed tannins in Lotus corniculatus. J Exp Bot 56: 1093–1103CrossRefPubMedGoogle Scholar
  11. Paolocci F, Robbins MP, Madeo L, Arcioni S, Damiani F (2006) The genetic control of the two branches of the pathway leading to the biosynthesis of proanthocyanidins in legume leaves. Proceedings of the 15th Italian Society of Agricultural Genetics, 10–14 September, Ishia, Italy, 2:02Google Scholar
  12. Paolocci F, Robbins MP, Madeo L, Arcioni S, Martens S, Damiani F (2007) Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis. Structure, expression analysis, and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus. Plant Physiol143: 504–516CrossRefPubMedGoogle Scholar
  13. Park K-I, Ishikawa N, Morita Y, Choi J-D, Hoshino A, Iida S (2007) A bHLH regulatory gene in the common morning glory, Ipomoea purpurea, controls anthocyanin biosynthesis in flowers, proanthocyanidins and phytomelanin pigmentation in seeds, and seed trichome formation. Plant J 49: 641–654CrossRefPubMedGoogle Scholar
  14. Perry JA, Wang TL, Welham TJ, Gardner S, Pike JM, Yoshida S, Parniske M (2004) A TILLING reverse genetics tool and a web-accessible collection of mutants of the legume Lotus japonicus. Plant Physiol 131: 866–871CrossRefGoogle Scholar
  15. Ray H, Yu M, Auser P, Blahut-Beatty L, McKersie B, Bowley S, Westcott N, Coulman B, Lloyd A, Gruber MY (2003) Expression of anthocyanins and proanthocyanidins after transformation of alfalfa with maize Lc. Plant Physiol 132: 1448–1463CrossRefPubMedGoogle Scholar
  16. Robbins MP, Paolocci F, Hughes JW, Turchetti V, Allison GG, Arcioni S, Morris P, Damiani F (2003) Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus corniculatus. J Exp Bot 54: 239–248CrossRefPubMedGoogle Scholar
  17. Stracke R, Werber M, Weisshaar B (2001) The R2R3-MYB family in Arabidopsis thaliana. Curr Opin Plant Biol 4: 447–456CrossRefPubMedGoogle Scholar
  18. Zhang JL (2003) Overexpression analysis of plant transcription factors. Curr Opin Plant Biol 6: 430–440CrossRefPubMedGoogle Scholar
  19. Zhang F, Gonzales A, Zhao M, Payne CT, Lloyd A (2003) A network of redundant bHLH proteins functions in all TTG1-dependent pathways of Arabidopsis. Development 130: 4859–4869CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science + Business Media, LLC 2009

Authors and Affiliations

  • Mark P. Robbins
    • 1
  • David Bryant
    • 1
  • Samantha Gill
    • 1
  • Phillip Morris
    • 1
  • Paul Bailey
    • 2
  • Tracey Welham
    • 2
  • Cathie Martin
    • 2
  • Trevor L. Wang
    • 2
  • Takakazu Kaneko
    • 3
  • Shusei Sato
    • 3
  • Satoshi Tabata
    • 3
  • Francesco Paolocci
    • 4
  1. 1.Biorenewables and Plant Cell Biology TeamInstitute of Grassland and Environmental Research, Aberystwyth Research CentreAberystwythUK
  2. 2.John Innes CentreColney LaneUK
  3. 3.Kazusa DNA Research InstituteKisarazuJapan
  4. 4.Institute of Plant Genetics- CNRResearch Division of PerugiaPerugiaItaly

Personalised recommendations