Protein–Protein Interactions Involving the N-Terminus of p35

  • Gary Kar Ho Ng
  • Lisheng He
  • Robert Z. Qi


As a major regulator of Cdk5, the protein p35 exerts various effects on neuronal cells. Under certain conditions, p35 undergoes truncation of its N-terminus to form the protein p25. Although p25 is equally potent in activating Cdk5 as p35, it displays a number of differences from the latter, indicating an indispensable role of the N-terminal region for carrying out many of the Cdk5-p35 activities. A number of proteins have been identified that interact with p35 via its N-terminus. Such binding confers p35 and Cdk5 a multitude of properties, including regulation of Cdk5 activity by interacting with the proteins SET, CK2, importins β, 5 and 7, as well as the microtubule cytoskeleton; mediation of nuclear import of p35 through binding with the importin proteins; and participation in the control of protein biogenesis by regulating ribosomal protein S6 kinase 1. p35 itself also functions as a microtubule-associated protein through its N-terminus to regulate microtubule dynamics which is required for neurite outgrowth. Therefore, the discovery of p35–N-terminal binding partners has facilitated the elucidation of p35 and Cdk5 functions and regulations.


Neurite Outgrowth Nuclear Import Cdk5 Activity Ser411 Phosphorylation Rabbit Reticulocyte Lysate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by the Earmarked Research Grant from the Research Grants Council and the Area of Excellence Scheme under the University Grants Committee of Hong Kong.


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© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  1. 1.Department of BiochemistryThe Hong Kong University of Science and TechnologyKowloonChina

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