Introduction to Bacterial Signal Transduction Networks

  • Yoko Eguchi
  • Ryutaro Utsumi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 631)


Transcriptional analysis using a DNA microarray is an extremely efficient method for analyzing two-component signal transduction networks. Here we introduce three such networks in Escherichia coli that were clarified using a DNA microarray: a PhoQ/PhoP system that senses extracellular Mg2+ and controls the gene expression for adaptation to environmental Mg2+ deprivation (Mg2+ stimulon); an, EvgS/EvgA signal transduction system that activates expression of multiple drug efflux pumps and acid resistance genes; and a signal transduction cascade between EvgS/EvgA and PhoQ/PhoP.


Histidine Kinase Signal Transduction Cascade Signal Transduction Network Cognate Response Regulator Phosphorelay System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    Mizuno T. Compilation of all genes encoding two-component phosphotransfer signal transducers in the genome of Escherichia coli. DNA Res 1997; 4:161–168.PubMedCrossRefGoogle Scholar
  2. 2.
    Oshima T, Aiba H, Masuda Y et al. Transcriptome analysis of all two-component regulatory system mutants of Escherichia coli K-12. Mol Microbiol 2002; 46:281–291.PubMedCrossRefGoogle Scholar
  3. 3.
    Minagawa S, Ogasawara H, Kato A et al. Identification and molecular characterization of the Mg2+-stimulon of Escherichia coli. J Bacteriol 2003; 185:3696–3702.PubMedCrossRefGoogle Scholar
  4. 4.
    Vescovi EG, Soncini FC, Groisman EA. Mg2+ as an extracellular signal: environmental regulation of Salmonella virulence. Cell 1996; 84:165–174.CrossRefGoogle Scholar
  5. 5.
    Soncini FC, Groisman EA. Transcriptional autoregulation of the Salmonella typhimurium phoPQ operon. J Bacteriol 1996; 177:4364–4371.Google Scholar
  6. 6.
    Soncini FC, Vescovi EG, Groisman EA. Two-component regulatory systems can interact to process multiple environmental signals. J Bacteriol 1996; 178:6796–6801.PubMedGoogle Scholar
  7. 7.
    Groisman EA. The pleiotropic two-component regulatory system PhoP-PhoQ. J Bacteriol 2001; 183:1835–1842.PubMedCrossRefGoogle Scholar
  8. 8.
    Utsumi R, Katayama, S, Taniguchi M et al. Newly identified genes involved in the signal transduction of Escherichia coli K-12. Gene 1994; 140:73–77.PubMedCrossRefGoogle Scholar
  9. 9.
    Perraud AL, Weiss V, Gross R. Signaling pathways in two-component phosphorelay systems. Trends Microbiol 1999; 7:115–120.PubMedCrossRefGoogle Scholar
  10. 10.
    Utsumi R. Bacterial signal transduction for environmental adaptation and their inhibitors. Microbes Environment 2002; 17:1–5.CrossRefGoogle Scholar
  11. 11.
    Bock A, Gross R. The unorthodox histidine kinases BvgS and EvgS are responsive to the oxidation status of a quinone electron carrier. Eur J Biochem 2002; 269:3479–3484.PubMedCrossRefGoogle Scholar
  12. 12.
    Georgellis D, Kwon O, Lin EC. Quinones as the redox signal for the are two-component system of bacteria. Science 2001; 292:2314–2316.PubMedCrossRefGoogle Scholar
  13. 13.
    Kato A, Ohnishi H, Yamamoto K et al. Transcription of emrKY is regulated by the EvgA-EvgS two-component system in Escherichia coli K-12. Biosci Biotechnol Biochem 2000; 64:1203–1209.PubMedCrossRefGoogle Scholar
  14. 14.
    Nishino K, Yamaguchi A. Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug-resistance transporters. J Bacteriol 2001; 183:1455–1458.PubMedCrossRefGoogle Scholar
  15. 15.
    Nishino K, Yamaguchi A. Analysis of a complete library of putative drug transporter genes in Escherichia coli. J Bacteriol 2001; 183:5803–5812.PubMedCrossRefGoogle Scholar
  16. 16.
    Masuda N, Church GM. Escherichia coli gene expression responsive to levels of the response regulator EvgA. J Bacteriol 2002; 184:6225–6234.PubMedCrossRefGoogle Scholar
  17. 17.
    Masuda N, Church GM. Regulatory network of acid resistance genes in Escherichia coli. Mol Microbiol 2003; 48:699–712.PubMedCrossRefGoogle Scholar
  18. 18.
    Eguchi Y, Oshima T, Mori H et al. Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli. Microbiology 2003; 149:2819–2828.PubMedCrossRefGoogle Scholar
  19. 19.
    Eguchi Y, Okada T, Minagawa S et al. Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli. J Bacteriol 2004; 186:3006–3014.PubMedCrossRefGoogle Scholar
  20. 20.
    Kato A, Groisman EA. Connecting two-component regulatory systems by a protein that protects a response regulator from dephosphorylation by its cognate sensor. Genes Dev 2004; 18:2302–2313.PubMedCrossRefGoogle Scholar
  21. 21.
    Eguchi Y, Itou J, Yamane M et al. B1500, a small membrane protein, connecting EvgS/EvgA and PhoQ/PhoP two component systems of Escherichia coli. Proc Natl Acad Sci USA; 2007; 104:18712–18717.PubMedCrossRefGoogle Scholar
  22. 22.
    Hagiwara D, Yamashino T, Mizuno T. A genome-wide view of the Escherichia coli BasS-BasR two-component system implicated in iron-response. Biosci Biotechnol Biochem 2004; 68:1758–1767.PubMedCrossRefGoogle Scholar
  23. 23.
    Hagiwara D, Sugiura M, Oshima T et al. Genome-wide analyses revealing a signal network of the RcsC-YojN-RcsB phosphorelay system in E. coli. J Bacteriol 2003; 185:5735–5746.PubMedCrossRefGoogle Scholar
  24. 24.
    Ogasawara H, Hasegawa A, Kanda E et al. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J Bacteriol 2007; 189:4791–4799.PubMedCrossRefGoogle Scholar

Copyright information

© Landes Bioscience and Springer Science+Business Media 2008

Authors and Affiliations

  1. 1.Department of Bioscience, Graduate School of AgricultureKinki UniversityNaraJapan

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