Enzyme Kinetics: Theory and Practice

  • Alistair Rogers
  • Yves Gibon

Enzymes, like all positive catalysts, dramatically increase the rate of a given reaction. Enzyme kinetics is principally concerned with the measurement and mathematical description of this reaction rate and its associated constants. For many steps in metabolism, enzyme kinetic properties have been determined, and this information has been collected and organized in publicly available online databases (www.brenda.uni-koeln.de). In the first section of this chapter, we review the fundamentals of enzyme kinetics and provide an overview of the concepts that will help the metabolic modeler make the best use of this resource. The techniques and methods required to determine kinetic constants from purified enzymes have been covered in detail elsewhere [4, 12] and are not discussed here. In the second section, we will describe recent advances in the high throughput, high sensitivity measurement of enzyme activity, detail the methodology, and discuss the use of high throughput techniques for profiling large numbers of samples and providing a first step in the process of identifying potential regulatory candidates.


Substrate Concentration Glutamine Synthetase Substrate Complex Adenylate Kinase Noncompetitive Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



A.R. acknowledges support from the U.S. Department of Energy (DOE), Office of Science, Biological and Environmental Research (BER) program as part of its Program for Ecosystem Research (PER) and contract No. DE-AC02-98CH10886 to Brookhaven National Laboratory. Y.G. acknowledges Mark Stitt, Melanie Höhne, Jan Hannemann, John Lunn, Hendrik Tschoep, Ronan Sulpice, Marie-Caroline Steinhauser, and support from the Max Planck Society and the German Ministry for Research and Technology (GABI 0313110).


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Alistair Rogers
    • 1
  • Yves Gibon
    • 2
  1. 1.Department of Environmental SciencesBrookhaven National LaboratoryUptonUSA
  2. 2.Max Planck Institute for Molecular Plant PhysiologyUniversity of Illinois at Urbana Champaign14059 Golm-PotsdamGermany

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