Porous Silicon Optical Label-Free Biosensors

  • Philippe M. Fauchet
Part of the Nanostructure Science and Technology book series (NST)


Because of its large internal surface area, porous silicon (PSi) is an attractive material for chemical and biological sensing applications. This chapter describes label-free optical biosensors consisting of PSi photonic bandgap structures. After a brief review of the material science and key properties of PSi, the design, fabrication, and sensitivity of various PSi photonic bandgap structures is discussed. The properties of one type of these structures, namely PSi microcavities, are discussed in detail. The sensitivity of these structures is then verified experimentally via the detection of a single monolayer of silane and glutaraldehyde. Examples of biosensing are then discussed. They include the detection of DNA segments, Gram negative bacteria, IgG, and pathogenic E. coli.


Porous Silicon Effective Refractive Index Porous Silicon Layer Mesoporous Silicon Bragg Mirror 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported in part by grants from the U.S. National Science Foundation and the Infotonics Center of Excellence in Canandaigua, N Y. The authors acknowledge the following individuals for their contributions: Prof. B. Miller, Dr. S. Chan, Dr. M. Christophersen, Dr. L. DeLouise, Dr. C. Striemer, and R. Viard.


  1. 1.
    J. Wang, Nucleic Acids Res., 28, 3001 (2000)Google Scholar
  2. 2.
    A. Brecht, G. Gauglitz, Sensor Actuators. B, 38, 1 (1997)CrossRefGoogle Scholar
  3. 3.
    E. Danelian, A. Karlen, R. Karlsson, S. Winiwarter, A. Hansson, S. Lofas, H. Lennernas, M. D. Hamalainen J. Med. Chem., 43, 2083 (2000)CrossRefGoogle Scholar
  4. 4.
    J. P. Clarkson, V. Rajalingam, K. D. Hirshman, H. Ouyang, W. Sun, P. M. Fauchet, Mat. Res. Soc. Symp. Proc., 869, D321 (2005)Google Scholar
  5. 5.
    M. Archer, M. Christophersen, P. M. Fauchet, Biomed. Microdevices, 6, 203 (2004)CrossRefGoogle Scholar
  6. 6.
    P. Bataillard, E. Steffgen, S. Haemmerli, A. Manz, H. M. Widmer, Biosens. Bioelectron., 8, 89 (1993)CrossRefGoogle Scholar
  7. 7.
    D. Zhang, G. M. Crean, T. Flaherty, A. Shallow, Analyst, 118, 429 (1993)CrossRefGoogle Scholar
  8. 8.
    T. Vo-Dinh, B. Cullum, Fresenius J. Anal. Chem., 366, 540, (2000)Google Scholar
  9. 9.
    A. Uhlir, Jr., Bell Syst. Tech. J., 35, 333 (1956)Google Scholar
  10. 10.
    Y. Watanabe, Y. Arita, T. Yokoyama, Y. Igarashi, J. Electrochem. Soc., 122, 1351 (1975)CrossRefGoogle Scholar
  11. 11.
    L. T. Canham, Appl. Phys. Lett., 57, 1046 (1990).CrossRefGoogle Scholar
  12. 12.
    K. D. Hirschmann, L. Tsybeskov, S. P. Duttagupta, P. M. Fauchet, Nature, 384, 338 (1996)CrossRefGoogle Scholar
  13. 13.
    M. G. Berger, C. Dieker, M. Thoenissen, L. Vescan H. Luth, H. Munder, J. Phys. D, 27, 1333 (1994)CrossRefGoogle Scholar
  14. 14.
    M. Araki, H. Koyama, N. Koshida, Appl. Phys. Lett., 68, 2999 (1996)CrossRefGoogle Scholar
  15. 15.
    M. P. Stewart, J. M. Buriak, Adv. Mater., 12, 859 (2000)CrossRefGoogle Scholar
  16. 16.
    L. T. Canham, in Properties of Porous Silicon, INSPEC London, UK, L. Canham editor, pp. 81–89 (1997)Google Scholar
  17. 17.
    J. M. Buriak, Chem. Commun., 12, 1051 (1999)CrossRefGoogle Scholar
  18. 18.
    B. Lillis, C. Jungk, D. Iacopino, A. Whelton, E. Hurley, M. M. Sheehan, A. Splinter, A. Quinn, G. Redmond, W. A. Lane, A. Mathewson, H. Berney, Biosens. Bioelectron., 21,282 (2005)CrossRefGoogle Scholar
  19. 19.
    S. M. Weiss, M. Lee, M. Molinari, H. Ouyang, P. M. Fauchet, Proc. of SPIE, 5511, 144 (2004)CrossRefGoogle Scholar
  20. 20.
    H. Ouyang, M. Christophersen, R. Viard, B. L. Miller, P. M. Fauchet, Adv. Funct. Mater., 15, 1851 (2005)CrossRefGoogle Scholar
  21. 21.
    M. Haurylau, A.R. Shroff, P.M. Fauchet, Phys. Stat. Sol. A, 202, 1477 (2005)CrossRefGoogle Scholar
  22. 22.
    P. M. Fauchet, Encyclopedia of Applied Physics, Update 2, Wiley-VCH Verlag, pp. 249–272 (1999)Google Scholar
  23. 23.
    R. L. Smith, S. D. Collins, J. Appl. Phys., 71, R1 (1992)CrossRefGoogle Scholar
  24. 24.
    A. G. Cullis, L. T. Canham, P. D. J. Calcott, J. Appl. Phys., 82, 909 (1997)CrossRefGoogle Scholar
  25. 25.
    V. Lehmann, Electrochemistry of Silicon, WILEY VCH, Verlag, GmbH (2002)Google Scholar
  26. 26.
    M. Thonissen, M. G. Berger, S. Billat, R. Ares-Fischer, M. Kruger, H. Luth, W. Theiss, S. Hillbrich, P. Grosse, G. Lerondel, U. Frotscher, Thin Solid Films, 297, 92 (1997)CrossRefGoogle Scholar
  27. 27.
    W.Theiss, Surface Science Reports, 29, 91 (1997)CrossRefGoogle Scholar
  28. 28.
    F. Muller, A. Birner, J. Schilling, U. Gosele, C. Kettner, P. Hanggi, Phys. Stat. Sol. A, 182, 585 (2000)CrossRefGoogle Scholar
  29. 29.
    W. Theiss, Thin Solid Films, 276, 7 (1996)CrossRefGoogle Scholar
  30. 30.
    C. L. Curtis, V. V. Doan, G. M. Credo, M. J. Sailor, J. Electrochem. Soc., 140, 3492, (1993)CrossRefGoogle Scholar
  31. 31.
    V. S.-Y Lin, K. Motesharei, K-P. S. Dancil, M. J. Sailor, M. R. Ghadiri, Science, 278, 840 (1997)CrossRefGoogle Scholar
  32. 32.
    K-P. S. Dancil, D. P. Greiner, M. J. Sailor, J. Am. Chem. Soc., 121, 7925, (1999)CrossRefGoogle Scholar
  33. 33.
    E. J. Anglin, M. P. Schwartz, V. P. Ng, L. A. Perelman, M. J. Sailor, Langmuir, 20, 11264 (2004)CrossRefGoogle Scholar
  34. 34.
    S. Chan, Y.Li, L. J. Rothberg, B. L. Miller, P. M. Fauchet, Mat. Sci. Eng. C, 15, 277 (2001)CrossRefGoogle Scholar
  35. 35.
    S. Chan, P. M. Fauchet, Y. Li, L. J. Rothberg, B. L. Miller, Phys. Stat. Sol. (a) 182, 541 (2000)CrossRefGoogle Scholar
  36. 36.
    S. Chan, S. R. Horner, P. M. Fauchet, B. L. Miller, J. Am. Chem. Soc., 123, 11797 (2001)CrossRefGoogle Scholar
  37. 37.
    V. Mulloni, L. Pavesi, Appl. Phys. Lett., 76, 2523 (2000)CrossRefGoogle Scholar
  38. 38.
    F. Cunin, T.A. Schmedake, J.R. Link, Y.Y. Li, J. Koh, S.N. Bhatia, M.J. Sailor, Nat. Mater., 1, 39 (2002)CrossRefGoogle Scholar
  39. 39.
    L. A. DeLouise, P. M. Fauchet, B. L. Miller, A. A. Pentland, Adv. Mater., 17, 2199 (2005)CrossRefGoogle Scholar
  40. 40.
    T. A. Schemedake, F. Cunin, J. R. Link, M. J. Sailor, Adv. Mater., 14, 1270 (2002)CrossRefGoogle Scholar
  41. 41.
    G. Di Francia, V. La Ferrara, L. Quercia, G. Faglia, J. Porous Mater., 7, 287 (2000)CrossRefGoogle Scholar
  42. 42.
    W. J. Jin, Gl L. Shen, R. Q. Yu, Spectrachim, Acta, A, 54, 1407 (1998)CrossRefGoogle Scholar
  43. 43.
    J. H. Song, M. J. Sailor, J. Am. Chem. Soc., 119, 7381 (1997)CrossRefGoogle Scholar
  44. 44.
    V. M. Starodub, L. L. Fedorenko, A. P. Sisetskiy, N. F. Starodub, Sensor Actuators B, 58, 409 (1999)CrossRefGoogle Scholar
  45. 45.
    V. Polishchuk, E. Souteyrand, J.R. Martin, V. I. Strikha, V. A. Skryshevshy, Anal. Chim. Acta., 375, 205 (1998)CrossRefGoogle Scholar
  46. 46.
    R. Angelucci, A. Poggi, L. Dori, G. C. Cardinali, A. Parisini, A. Tagliani, M. Mariasaldi, F. Cavani, Sensor Actuators, 74, 95 (1999)CrossRefGoogle Scholar
  47. 47.
    R. W. Bogue, Biosensors Bioelectronics, 12, xxvii (1977)CrossRefGoogle Scholar
  48. 48.
    M. Ben Ali, R. Mika, H. Ben Ouada, R. Mghaieth, H. Maaref, Sensor. Actuators, 74, 123, (1999)CrossRefGoogle Scholar
  49. 49.
    T. F. Krauss, R. M. De la Rue, Progress in Quantum Electronics, 23, 51 (1999)CrossRefGoogle Scholar
  50. 50.
    E. Chow, A. Grot, L. W. Mirkarimi, M. Sigalas, G. Girolami, Opt. Lett., 29, 1093 (2004)CrossRefGoogle Scholar
  51. 51.
    M. Loncar, A. Scherer, Appl. Phys. Lett., 82, 4648 (2003)CrossRefGoogle Scholar
  52. 52.
    J. Topolancik, P. Bhattacharya, J. Sabarinathan, P.C. Yu, Appl. Phys. Lett., 82, 1143 (2003)CrossRefGoogle Scholar
  53. 53.
    K. J. Vahala, Nature, 424,839 (2003)CrossRefGoogle Scholar
  54. 54.
    Y. J. Lee, P. V. Braun, Adv. Mater., 15,563 (2003)CrossRefGoogle Scholar
  55. 55.
    A. Birner, R. B. Wehrspohn, U. M. Gosele, K. Busch, Adv. Mater., 13, 377 (2000)CrossRefGoogle Scholar
  56. 56.
    L. Pavesi, La Rivista del Nuovo Cimento, 20,1 (1997)CrossRefGoogle Scholar
  57. 57.
    E. Hecht, Optics, Addison-Wesley Publishing Company, Menlo Park, CA, (1979)Google Scholar
  58. 58.
    T. Ruckstuhl, M. Rankl, S. Seeger, Biosensors Bioelectronics, 18, 1193 (2003)CrossRefGoogle Scholar
  59. 59.
    J. S. Shumaker-Parry, C. T. Campbell, Anal. Chem., 76, 907 (2004)CrossRefGoogle Scholar
  60. 60.
    P. Chen, F. C. J. Mao, Food: Drug Anal., 12, 133 (2004)Google Scholar
  61. 61.
    N. Nath, A. Chilkoti, Anal. Chem., 75, 5370 (2004)CrossRefGoogle Scholar
  62. 62.
    V. S.-Y Lin, K. Motesharei, K-P. S. Dancil, M. J. Sailor, M. R. Ghadiri, Science, 278, 840 (1997)CrossRefGoogle Scholar
  63. 63.
    H. Ouyang, M. Lee, B. L. Miller, P. M. Fauchet, Proc. of SPIE, 5926, 59260J (2005)CrossRefGoogle Scholar
  64. 64.
    H. Ouyang, P. M. Fauchet, Proc. of SPIE, 6005, 600508–1 (2005)CrossRefGoogle Scholar
  65. 65.
    P. J. Reece, G. Lérondel, W. H. Zheng, M. Gal, Appl. Phys. Lett., 81, 4895 (2002)CrossRefGoogle Scholar
  66. 66.
    H. Ouyang, M. Christophersen, P. M. Fauchet, Phys. Stat. Sol. A, 202, 1396 (2005)CrossRefGoogle Scholar
  67. 67.
    H. Ouyang, C. C. Striemer, P. M. Fauchet, Appl. Phys. Lett., 88, 163108 (2006)CrossRefGoogle Scholar
  68. 68.
    G. Hermanson, Biocojugate Techniques, Academic Press, New York (1996)Google Scholar
  69. 69.
    L. Tsybeskov, S. P. Duttagupta, K. D. Hirschman, P. M. Fauchet, Appl. Phys. Lett., 68, 2058 (1996)CrossRefGoogle Scholar
  70. 70.
    L. S.Young, W. J. Martin, R. D. Meyer, R. J. Weinstein, E. T. Anderson, Ann. Inern. Med., 86, 456 (1997)Google Scholar
  71. 71.
    R. D. Hubbard, S. R. Horner, B. L. Miller, J. Am. Chem. Soc., 123, 5811 (2001)CrossRefGoogle Scholar
  72. 72.
    L. S. Jung, K. E. Nelson, P.S. Stayton, C.T. Campbell, Langmuir 16, 94221 (2000)Google Scholar
  73. 73.
    E. O. Saphire, P. W. H. I. Parren, R. Pantophlet, M. B. Zwick, G. M. Morris, P. M. Rudd, R. A. Dwek, R. L. Stanfield, D. R. Burton, I. A. Wilson, Science, 293, 1155 (2001)CrossRefGoogle Scholar
  74. 74.
    Zaharik, M. L.; Gruenheid, S.; Perrin, A. J.; Finlay, B. B. Int. J. Med. Microb., 291, 593 (2002) CrossRefGoogle Scholar
  75. 75.
    Horner, S. R.; Mace, C. R.; Rothberg, L. J.; Miller, B. L. Biosens. Bioelectron., 21, 1659 (2006)CrossRefGoogle Scholar
  76. 76.
    P. Schuck, Annu. Rev. Biophys. Biomol. Struct., 26, 541 (1997)CrossRefGoogle Scholar
  77. 77.
    L. S. Jung, K. E. Nelson, P.S. Stayton, C. T. Campbell, Langmuir, 16, 9421(2000)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Philippe M. Fauchet
    • 1
  1. 1.University of RochesterRochesterUSA

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