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Host Cell Actin Remodeling in Response to Trypanosoma cruzi: Trypomastigote Versus Amastigote Entry

  • Chapter
Molecular Mechanisms of Parasite Invasion

Part of the book series: Subcellular Biochemistry ((SCBI,volume 47))

Abstract

Trypanosoma cruzi is the protozoan parasite that causes Chagas’ disease, a highly prevalent vector-borne disease in Latin America. Chagas’ disease is a major public health problem in endemic regions with an estimated 18 million people are infected with T. cruzi and another 100 million at risk (http://www.who.int/ctd/chagas/disease.htm). During its life cycle, T. cruzi alternates between triatomine insect vectors and mammalian hosts. While feeding on host’s blood, infected triatomines release in their feces highly motile and infective metacyclic trypomastigotes that may initiate infection. Metacyclic trypomastigotes promptly invade host cells (including gastric mucosa) and once free in the cytoplasm, differentiate into amastigotes that replicate by binary fission. Just before disruption of the parasite-laden cell, amastigotes differentiate back into trypomastigotes which are then released into the tissue spaces and access the circulation. Circulating trypomastigotes that disseminate the infection in the mammalian host may be taken up by feeding triatomines and may also transform, extracellu-larly, into amastigote-like forms.1 Unlike their intracellular counterparts, these amastigote-like forms,2 henceforth called amastigotes, are capable of infecting host cells.3–7 Studies in which the mechanisms of amastigote invasion of host cells have been compared to metacyclic trypomastigote entry have revealed interesting differences regarding the involvement of the target cell actin microfilament system.

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References

  1. Burleigh BA, Andrews NW. The mechanisms of Trypanosoma cruzi invasion of mammalian cells. Annu Rev Microbiol 1995; 49:175–200.

    Article  PubMed  CAS  Google Scholar 

  2. Fernandes AB, Mortara RA. Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages. Microbes Infect 2004; 6:460–467.

    Article  PubMed  CAS  Google Scholar 

  3. Ley V, Andrews NW, Robbins ES et al. Amastigotes of Trypanosoma cruzi sustain an infective cycle in mammalian cells. J Exp Med 1988; 168:649–659.

    Article  PubMed  CAS  Google Scholar 

  4. Pan SCT. Trypanosoma cruzi: In vitro interactions between cultured amastigotes and human skin-muscle cells. Exp Parasitol 1978; 45:274–286.

    Article  PubMed  CAS  Google Scholar 

  5. Behbehani K. Developmental cycles of Trypanosoma (Schyzotrypanum) cruzi (Chagas, 1909) in mouse peritoneal macrophages in vitro. Parasitology 1973; 66:343–353.

    Article  PubMed  CAS  Google Scholar 

  6. Nogueira N, Cohn Z. Trypanosoma cruzi: Mechanism of entry and intracellular fate in mammalian cells. J Exp Med 1976; 143:1402–1420.

    Article  PubMed  CAS  Google Scholar 

  7. Mortara RA. Trypanosoma cruzi: Amastigotes and trypomastigotes interact with different structures on the surface of HeLa cells. Exp Parasitol 1991; 73:1–14.

    Article  PubMed  CAS  Google Scholar 

  8. Frischknecht F, Way M. Surfing pathogens and the lessons learned for actin polymerization. Trends Cell Biol 2001; 11:30–38.

    Article  PubMed  CAS  Google Scholar 

  9. Cossart P, Sansonetti PJ. Bacterial invasion: The paradigms of enteroinvasive pathogens. Science 2004; 304:242–248.

    Article  PubMed  CAS  Google Scholar 

  10. Bourdet-Sicard R, Egile C, Sansonetti PJ et al. Diversion of cytoskeletal processes by Shigella during invasion of epithelial cells. Microbes Infect 2000; 2(7):813–819.

    Article  PubMed  CAS  Google Scholar 

  11. Cossart P. Subversion of the mammalian cell cytoskeleton by invasive bacteria. J Clin Invest 1997; 99:2307–2311.

    Article  PubMed  CAS  Google Scholar 

  12. Dramsi S, Cossart P. Intracellular pathogens and the actin cytoskeleton. Annu Rev Cell Dev Biol 1998; 14:137–166.

    Article  PubMed  CAS  Google Scholar 

  13. Goosney DL, Gruenheid S, Finlay BB. Gut feelings: Enteropathogenic E. coli (EPEC) interactions with the host. Annu Rev Cell Dev Biol 2000; 16:173–189.

    Article  PubMed  CAS  Google Scholar 

  14. Silva MLM, Mortara RA, Barros HC et al. Aggregation of membrane-associated actin filaments following localized adherence of enteropathogenic Escherichia coli to HeLa cells. J Cell Sci 1989; 93:439–446.

    PubMed  Google Scholar 

  15. Hudson L, Snary D, Morgan SJ. Trypanosoma cruzi: Continuous cultivation with murine cell lines. Parasitology 1984; 88:283–294.

    PubMed  Google Scholar 

  16. Procópio DO, Barros HC, Mortara RA. Actin-rich structures formed during the invasion of cultured cells by infective forms of Trypanosoma cruzi. Eur J Cell Biol 1999; 78:911–924.

    PubMed  Google Scholar 

  17. Rosenshine I, Finlay BB. Exploitation of host signal transduction pathways and cytoskeletal functions by invasive bacteria. BioEssays 1993; 15:17–24.

    Article  PubMed  CAS  Google Scholar 

  18. Silva CV, Luquetti AO, Rassi A et al. Involvement of Ssp-4-related carbohydrate epitopes in mammalian cell invasion by Trypanosoma cruzi amastigotes. Microbes Infect 2006, (in press).

    Google Scholar 

  19. Schenkman S, Andrews NW, Nussenzweig V et al. Trypanosoma cruzi invade a mammalian epithelial cell in a polarized manner. Cell 1988; 55:157–165.

    Article  PubMed  CAS  Google Scholar 

  20. Schenkman S, Mortara RA. HeLa cells extend and internalize pseudopodia during active invasion by Trypanosoma cruzi trypomastigotes. J Cell Sci 1992; 101:895–905.

    PubMed  Google Scholar 

  21. Barbosa HS, Meirelles MNL. Evidence of participation of cytoskeleton of heart muscle cells during the invasion of Trypanosoma cruzi. Cell Struct Funct 1995; 20:275–284.

    Article  PubMed  CAS  Google Scholar 

  22. Procópio DO, Silva S, Cunningham CC et al. Trypanosoma cruzi: Effect of protein kinase inhibitors and cytoskeletal protein organization and expression on host cell invasion by amastigotes and metacyclic trypomastigotes. Exp Parasitol 1998; 90:1–13.

    Article  PubMed  Google Scholar 

  23. Clerc P, Sansonetti PJ. Entry of Shigella flexneri into HeLa cells: Evidence for directed phagocytosis involving actin polymerization and myosin accumulation. Infect Immun 1987; 55:2681–2688.

    PubMed  CAS  Google Scholar 

  24. Tran VN, Bourdet-Sicard R, Dumenil G et al. Bacterial signals and cell responses during Shigella entry into epithelial cells. Cell Microbiol 2000; 2:187–193.

    Article  Google Scholar 

  25. Andrews NW. From lysosomes into the cytosol: the intracellular pathway of Trypanosoma cruzi. Braz J Med Biol Res 1994; 27:471–475.

    PubMed  CAS  Google Scholar 

  26. Tardieux I, Webster P, Ravesloot J et al. Lysosome recruitment and fusion are early events required for trypanosome invasion of mammalian cells. Cell 1992; 71:1117–1130.

    Article  PubMed  CAS  Google Scholar 

  27. Schenkman S, Robbins ES, Nussenzweig V. Attachment of Trypanosoma cruzi to mammalian cells requires parasite energy, and invasion can be independent of the target cell cytoskeleton. Infect Immun 1991; 59:645–654.

    PubMed  CAS  Google Scholar 

  28. Kipnis TL, Calich VL, Dias da Silva W. Active entry of bloodstream forms of Trypanosoma cruzi into macrophages. Parasitology 1979; 78:89–98.

    PubMed  CAS  Google Scholar 

  29. Meirelles MNL, Araujo-Jorge TC, De Souza W. Interaction of Trypanosoma cruzi with macrophages in vitro: Dissociation of the attachment and internalization phases by low temperature and cytochalasin B. Z Parasitenkd 1982; 68:7–14.

    Article  PubMed  Google Scholar 

  30. Woolsey AM, Burleigh BA. Host cell actin polymerization is required for cellular retention of Trypanosoma cruzi and early association with endosomal/lysosomal compartments. Cell Microbiol 2004; 6:829–838.

    Article  PubMed  CAS  Google Scholar 

  31. Hartwig JH, Stossel TP. Isolation and properties of actin, myosin, and a new actin binding protein in rabbit alveolar macrophages. J Biol Chem 1975; 250:5696–5705.

    PubMed  CAS  Google Scholar 

  32. Hartwig JH, Tyler J, Stossel TP. Actin-binding protein promotes the bipolar and perpendicular branching of actin filaments. J Cell Biol 1980; 87:841–848.

    Article  PubMed  CAS  Google Scholar 

  33. Cunningham CC, Gorlin JB, Kwiatkowski DJ et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 1992; 255:325–327.

    Article  PubMed  CAS  Google Scholar 

  34. Cunningham CC, Stossel TP, Kwiatkowski DJ. Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin. Science 1991; 251:1233–1236.

    Article  PubMed  CAS  Google Scholar 

  35. Ridley AJ, Hall A. The small GTP-binding protein Rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell 1992; 70:389–399.

    Article  PubMed  CAS  Google Scholar 

  36. Ridley AJ, Paterson HF, Johnston CL et al. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 1992; 70:401–410.

    Article  PubMed  CAS  Google Scholar 

  37. Hall A. Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol 1994; 10:31–54.

    Article  PubMed  CAS  Google Scholar 

  38. Nobes CD, Hall A. Rho, Rac, and Cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell 1995; 81:53–62.

    Article  PubMed  CAS  Google Scholar 

  39. Kozma R, Ahmed S, Best A et al. The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts. Mol Cell Biol 1995; 15:1942–1952.

    PubMed  CAS  Google Scholar 

  40. Aktories K, Barbieri JT. Bacterial cytotoxins: Targeting eukaryotic switches. Nat Rev Microbiol 2005; 3:397–410.

    Article  PubMed  CAS  Google Scholar 

  41. Jou TS, Nelson WJ. Effects of regulated expression of mutant RhoA and Rac1 small GTPases on the development of epithelial (MDCK) cell polarity. J Cell Biol 1998; 142:85–100.

    Article  PubMed  CAS  Google Scholar 

  42. Criss AK, Ahlgren DM, Jou TS et al. The GTPase Racl selectively regulates Salmonella invasion at the apical plasma membrane of polarized epithelial cells. J Cell Sci 2001; 114:1331–1341.

    PubMed  CAS  Google Scholar 

  43. Cortez M, Atayde V, Yoshida N. Host cell invasion mediated by Trypanosoma cruzi surface molecule gp82 is associated with F-actin disassembly and is inhibited by enteroinvasive Escherichia coli. Microbes Infect 2006; 8:1502–1512.

    Article  PubMed  CAS  Google Scholar 

  44. Barros HC, Silva S, Verbisck NV et al. Release of membrane-bound trails by Trypanosoma cruzi amastigotes onto modified surfaces and mammalian cells. J Eukaryot Microbiol 1996; 43:275–285.

    Article  PubMed  CAS  Google Scholar 

  45. Mortara RA, Procópio DO, Barros HC et al. Features of host cell invasion by different infective forms of Trypanosoma cruzi. Mem Inst Oswaldo Cruz 1999; 94(Suppl 1):135–137.

    PubMed  Google Scholar 

  46. Neira I, Ferreira AT, Yoshida N. Activation of distinct signal transduction pathways in Trypanosoma cruzi isolates with differential capacity to invade host cells. Int J Parasitol 2002; 32:405–414.

    Article  PubMed  CAS  Google Scholar 

  47. Fernandes AB, Neira I, Ferreira AT et al. Cell invasion by Trypanosoma cruzi amastigotes of distinct infectivities: Studies on signaling pathways. Parasitol Res 2006, (in press).

    Google Scholar 

  48. Stecconi-Silva RB, Andreoli WK, Mortara RA. Parameters affecting cellular invasion and escape from the parasitophorous vacuole by different infective forms of Trypanosoma cruzi. Mem Inst Oswaldo Cruz 2003; 98:953–958.

    Article  PubMed  CAS  Google Scholar 

  49. Braga MV, De Souza W. Effects of protein kinase and phosphatidylinositol-3 kinase inhibitors on growth and ultrastructure of Trypanosoma cruzi. FEMS Microbiol Lett 2006; 256:209–216.

    Article  PubMed  CAS  Google Scholar 

  50. Andrade LO, Andrews NW. Lysosomal fusion is essential for the retention of Trypanosoma cruzi inside host cells. J Exp Med 2004; 200:1135–1143.

    Article  PubMed  CAS  Google Scholar 

  51. Woolsey AM, Sunwoo L, Petersen CA et al. Novel PI 3-kinase-dependent mechanisms of trypano-some invasion and vacuole maturation. J Cell Sci 2003; 116:3611–3622.

    Article  PubMed  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Disciplina de Parasitologia, Departamento de Microbiologia, Imunologia e Parasitologia, UNIFESP, Escola Paulista de Medicina, Rua Botucatu, 862, 6o andar, 04023-062, São Paulo, SP, Brazil

    Renato A. Mortara, Walter K. Andreoli, Maria Cecília D. C. Fernandes, Claudio V. da Silva, Adriana B. Fernandes, Carolina L’Abbate & Solange da Silva

Authors
  1. Renato A. Mortara
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  2. Walter K. Andreoli
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  3. Maria Cecília D. C. Fernandes
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  4. Claudio V. da Silva
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  5. Adriana B. Fernandes
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  6. Carolina L’Abbate
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  7. Solange da Silva
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Correspondence to Renato A. Mortara .

Editor information

Editors and Affiliations

  1. Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts, USA

    Barbara A. Burleigh PhD

  2. Department of Biological Sciences, Imperial College, London, UK

    Dominique Soldati-Favre PhD

  3. Département de Microbiologie et Medecine Moléculaire, Université de Genève, Geneve, Switzerland

    Dominique Soldati-Favre PhD

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© 2008 Landes Bioscience and Springer Science+Business Media

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Mortara, R.A. et al. (2008). Host Cell Actin Remodeling in Response to Trypanosoma cruzi: Trypomastigote Versus Amastigote Entry. In: Burleigh, B.A., Soldati-Favre, D. (eds) Molecular Mechanisms of Parasite Invasion. Subcellular Biochemistry, vol 47. Springer, New York, NY. https://doi.org/10.1007/978-0-387-78267-6_8

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