Abstract
Image rendering in confocal microscopy is a digital technique. To optimize confocal image acquisition, the operator needs to have a basic understanding of what constitutes a digital image and how it can be employed effectively to help visualize specific details. In this chapter, we will introduce the topic of digital images, discuss the basic components of the digital image, and provide some basics on how to optimize collection of these components with regard to confocal microscopy.
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Notes
- 1.
 In microscopy, the Nyquist–Shannon sampling theorem (Shannon 1998) tells us that we cannot accurately detect microscopic information smaller than 2 detector pixels by 2 detector pixels. This is related to the variability in signal collection and not specifically digital imaging, so we will ignore this signal processing issue until the discussion of detectors in Chap. 7.
Reference
Shannon, C. 1998. Communication in the presence of noise. Proc IEEE. 86:447–457.
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Jerome, W.G. (2011). Introduction to Digital Imaging for Confocal Microscopy. In: Price, R., Jerome, W. (eds) Basic Confocal Microscopy. Springer, New York, NY. https://doi.org/10.1007/978-0-387-78175-4_6
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DOI: https://doi.org/10.1007/978-0-387-78175-4_6
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