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Introduction to Digital Imaging for Confocal Microscopy

Chapter
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Abstract

Image rendering in confocal microscopy is a digital technique. To optimize confocal image acquisition, the operator needs to have a basic understanding of what constitutes a digital image and how it can be employed effectively to help visualize specific details. In this chapter, we will introduce the topic of digital images, discuss the basic components of the digital image, and provide some basics on how to optimize collection of these components with regard to confocal microscopy.

Keywords

Histogram Histogram stretch Image formats Gamma Look-up-table Pixel Resolution Voxel 

Reference

  1. Shannon, C. 1998. Communication in the presence of noise. Proc IEEE. 86:447–457.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.Department of PathologyVanderbilt University Medical CenterSouth NashvilleUSA

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