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Fluorescence Microscopy

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Basic Confocal Microscopy

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Abstract

To harness the power of fluorescence for biological microscopic imaging, a number of additional components must be introduced to the standard light microscope. The most important modifications are (1) a strong illumination system that provides suitable wavelengths for exciting a specific fluorochrome, (2) some mechanism to limit the illumination beam to specific wavelengths so that only the fluorochrome of choice is excited, and (3) a means to image only the light emitted from the fluorochrome so that the excitation light (and other stray wavelengths) does not degrade the image. In this chapter, we will discuss a basic set-up that meets these criteria. Subsequent chapters will expand on this theme and discuss additional modifications required for laser scanning and spinning-disk confocal fluorescence.

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References

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Author information

Authors and Affiliations

  1. Department of Pathology, Vanderbilt University Medical Center, U-2206 MCN, 1161 21st Ave, South Nashville, TN, 37232-2561, USA

    W. Gray (Jay) Jerome

Authors
  1. W. Gray (Jay) Jerome
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  2. Robert L. Price
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Correspondence to W. Gray (Jay) Jerome .

Editor information

Editors and Affiliations

  1. School of Medicine, Dept. Cell & Developmental, University of South Carolina, Garners Ferry Rd. 6311, Columbia, 29209, South Carolina, USA

    Robert L. Price

  2. Vanderbilt University Medical Center, 21st Avenue S. 1161, Nashville, 37232-2561, Tennessee, USA

    W. Gray (Jay) Jerome

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Jerome, W.G., Price, R.L. (2011). Fluorescence Microscopy. In: Price, R., Jerome, W. (eds) Basic Confocal Microscopy. Springer, New York, NY. https://doi.org/10.1007/978-0-387-78175-4_3

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