Plant genetic transformation technologies have brought fundamental changes to both plant biology laboratory research as well as to modern agricultural field practices. Once a recalcitrant plant for tissue culture and gene delivery, maize is becoming one of the most targeted cereal crops using genetic transformation for both basic and applied purposes. This chapter provides a brief review of the history of maize transformation technology development, but focuses extensively on technical aspects of the methodology, including DNA delivery systems, target tissues and genotypes, selectable markers for transformation, and various issues related to integration and expression of transgenes. Some recent observations and improvements from two maize transformation groups are discussed. It is anticipated that increasing genomics information will assist further enhancement of maize transformation technology leading to more rapid progress in understanding and improvement of this important crop.
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Wang, K., Frame, B., Ishida, Y., Komari, T. (2009). Maize Transformation. In: Bennetzen, J.L., Hake, S. (eds) Handbook of Maize. Springer, New York, NY. https://doi.org/10.1007/978-0-387-77863-1_31
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