A brief history of methods used to elucidate protein function, protein presence, and RNA transcript presence is provided. Gene expression profiling through microarray hybridization, high throughput sequencing, or quantitative reverse transcriptase-polymerase chain reaction methods are reviewed and compared. Proteomics analysis using two dimensional gel electrophoresis followed by protein identification by mass spectrometry is then discussed. Relative costs and prospects for future improvements are also presented.
KeywordsGene Expression Analysis Shoot Apical Meristem Transcriptome Profile Laser Microdissection Maize Gene
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