Long before recombinant DNA technology was invented, maize genetics was a vibrant and exciting science dominated by controlling elements, cytogenet-ics, gene mapping and heterosis. Genes were understood as mutationally-defined units of function that could be placed on chromosomes. Incorporation of the concept of DNA as genetic material and the central dogma of genetics (DNA ⇔ RNA ⇒ protein) into the thinking of maize geneticists occurred rapidly. But, the only way to propagate maize DNA was to plant a seed.
In this chapter, we provide a personal account of how maize molecular genetics came into existence. We focus on some of the original, urgent questions of maize genetics that required the tools of molecular biology for satisfying explanations. No pretense is implied concerning the completeness of the narrative below, and we emphasize that this is a personal account.
KeywordsTransposable Element Alcohol Dehydrogenase Maize Endosperm Intragenic Recombination Zein Protein
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- Doring, H. P., and Starlinger, P. (1986) Molecular genetics of transposable elements in plants. Ann. Rev. of Genet. 20, 175–200Google Scholar
- Freeling, M., and Bennett, D. C. (1985) Maize Adhl. Ann. Rev. of Genet. 19, 297–323Google Scholar
- McClintock, B. (1965) The control of gene action in maize. Brookhaven Sym. in Biology 18, 162–184Google Scholar
- Schwartz, D., (1959) Genetic studies on enzymes in maize and endosperm. Science 159, 1287Google Scholar
- Schwartz, D., (1960A) Electrophoretic and immunochemical studies with endosperm proteins of maize mutants. Genetics 45, 1419–1427Google Scholar
- Schwartz, D., (1960B) Genetic studies on mutant enzymes in maize: Synthesis of hybrid enzymes by heterozygotes. Genetics 16, 1210–1215Google Scholar
- Schwartz, D., (1963) Genetic studies on mutant enzymes in maize. I V. Comparison of pH 7.5 esterases synthesized in seedling and endosperm. Genetics 49, 373–377Google Scholar