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Handbook of Maize pp 523–538Cite as

Genetic Analyses with Oat-Maize Addition and Radiation Hybrid Lines

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Oat-maize addition lines, with individual maize (Zea mays L.) chromosomes added to the oat (Avena sativa L.) genome via wide hybridization and embryo rescue, simplify the maize genome by 10-fold. Radiation hybrids, derived through gamma irradiation of monosomic addition lines, have less than a complete maize chromosome in an oat genomic background. Maize genes and gene families can be readily located to their respective physical chromosome or chromosome segment by rapid, high-throughput, and straight-forward PCR experiments. Polymorphisms are not required to map a maize DNA fragment with this system. Numerous non-mapping uses of these lines currently underway are described. Seed and/or DNA of the addition lines in various oat and maize backgrounds are available as well as approximately 650 radiation hybrid lines.

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Authors and Affiliations

  1. Department of Agronomy and Plant Genetics and Microbial and Plant Genomics Institute, University of Minnesota, USA

    Ronald L. Phillips

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  1. Ronald L. Phillips
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  2. Howard W. Rines
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Correspondence to Ronald L. Phillips .

Editor information

Editors and Affiliations

  1. Department of Genetics, University of Georgia, Athens, GA, 30602–7223, USA

    Jeffrey L. Bennetzen

  2. USDA Plant Gene Expression Center, 800 Buchanan Street, Albany, CA, 94710, USA

    Sarah Hake

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Phillips, R.L., Rines, H.W. (2009). Genetic Analyses with Oat-Maize Addition and Radiation Hybrid Lines. In: Bennetzen, J.L., Hake, S. (eds) Handbook of Maize. Springer, New York, NY. https://doi.org/10.1007/978-0-387-77863-1_26

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