Genetic Analyses with Oat-Maize Addition and Radiation Hybrid Lines

Oat-maize addition lines, with individual maize (Zea mays L.) chromosomes added to the oat (Avena sativa L.) genome via wide hybridization and embryo rescue, simplify the maize genome by 10-fold. Radiation hybrids, derived through gamma irradiation of monosomic addition lines, have less than a complete maize chromosome in an oat genomic background. Maize genes and gene families can be readily located to their respective physical chromosome or chromosome segment by rapid, high-throughput, and straight-forward PCR experiments. Polymorphisms are not required to map a maize DNA fragment with this system. Numerous non-mapping uses of these lines currently underway are described. Seed and/or DNA of the addition lines in various oat and maize backgrounds are available as well as approximately 650 radiation hybrid lines.


Radiation Hybrid Maize Genome Crown Rust Maize Chromosome First Division Restitution 


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  1. Amarillo FIE, and Bass HW (2007) A transgenomic cytogenetic sorghum (Sorghum propinquum) bacterial artificial chromosome fluorescence in situ hybridization map of maize (.Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion. Genetics 177:1509–1526PubMedCrossRefGoogle Scholar
  2. Ananiev EV, Chamberlin MA, Gordon-Kamm WJ, Svitashev S, Wu C (2007) Artificial plant minichromosomes. US Patent Publication 20070271629Google Scholar
  3. Ananiev, E V, Phillips, RL and Rines, HW (1998a) Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci USA 95:13073–13078CrossRefGoogle Scholar
  4. Ananiev E V, Phillips RL, Rines HW (1998b) A knob-associated tandem repeat in maize capable of forming fold-back DNA segments: Are chromosome knobs megatransposons? Proc Natl Acad Sci USA 95:10785–10790CrossRefGoogle Scholar
  5. Ananiev E V, Phillips RL, Rines HW (1998c) Complex structure of knob DNA on maize chromosome 9: Retrotransposon invasion into heterochromatin. Genetics 149:2025–2037Google Scholar
  6. Ananiev E V, Riera-Lizarazu O, Rines HW, Phillips RL (1997) Oat-maize chromosome addition lines: A new system for mapping the maize genome. Proc Natl Acad Sci USA 94:3524–3529PubMedCrossRefGoogle Scholar
  7. Armstrong CL, Phillips RL (1988) Genetic and cytogenetic variation in plants regenerated from organogenic and friable, embryogenic tissue cultures of maize. Crop Sci 28:363–369Google Scholar
  8. Bass HW, Riera-Lizarazu O, Ananiev EV, Bordoli SJ, Rines HW, Phillips RL, Sedat JW, Agard DA, Cande WZ (2000) Evidence for the coincident initiation of homolog pairing and synapsis during the telomere-clustering (bouquet) stage of meiotic prophase. J Cell Sci 113:1033–1042PubMedGoogle Scholar
  9. Cabral C, Springer N, Rines HW, Phillips R (2007) Many maize genes are expressed in an oat background carrying a specific maize chromosome. 49th Annual Maize Genet Conf, St. Charles, IL Abstracts p 112Google Scholar
  10. Chen ZJ, Phillips RL, Rines HW (1998) Maize DNA enrichment by representational difference analysis. Theor Appl Genet 97:337–344CrossRefGoogle Scholar
  11. Cox DR, Burmeister M, Price ER, Kim S, Myers RM (1990) Radiation hybrid mapping: A somatic cell genetic method for constructing high-resolution maps of mammalian chromosomes. Science 250:245–250PubMedCrossRefGoogle Scholar
  12. Davis DW (1992) Characterization of oat haploids and their progeny. University of Minnesota MS ThesisGoogle Scholar
  13. Fox SL, Jellen EN, Kianian SF, Rines HW, Phillips RL (2001) Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat. Theor Appl Genet 102:320–326CrossRefGoogle Scholar
  14. Jellen EN, Rines HW, Fox SL, Davis DW, Phillips RL, Gill BS (1997) Characterization of “Sun II” oat monosomics through C-banding and identification of eight new “Sun II” monosomics. Theor Appl Genet 95:1190–1195CrossRefGoogle Scholar
  15. Jin W, Melo JR, Nagaki K, Talbert PB, Henikoff S, Dawe RK, Jiang J (2004) Maize centromeres: organization and functional adaptation in the genetic background of oat. Plant Cell 16:571–581PubMedCrossRefGoogle Scholar
  16. Koumbaris GL, Bass HW (2003) A new single-locus cytogenetic mapping system for maize (Zea mays L.): Overcoming FISH detection limits with marker-selected sorghum (S. propinquum L.) BAC clones. Plant J 35:647–659PubMedCrossRefGoogle Scholar
  17. Kowles RV, Walch MD, Minnerath JM, Bernacchi CJ, Stec AO, Rines HW, Phillips RL (2008) Expression of C4 photosynthesis enzymes in oat-maize chromosome addition lines. Maydica 53:69–78Google Scholar
  18. Kynast RG, Galatowitxch MW, Hanson L, Huettl PA, Lupke L, Phillips RL, Rines HW (2008) Maternal and paternal transmission to offspring of B-chromosomes of .Zea mays L. in the alien genetic background of Avena sativa L. Maize Genet Coop Newsl 82:23Google Scholar
  19. Kynast RG, Galatowitsch MW, Huettl PA, Phillips RL, Rines HW (2007) Adding B-chromosomes of Zea mays L. to the genome of Avena sativa L. Maize Genet Coop Newsl 81:6Google Scholar
  20. Kynast RG, Okagaki RJ, Galatowitsch MW, Granath SR, Jacobs MS, Stec AO, Rines HW, Phillips RL (2004) Dissecting the maize genome by using chromosome addition and radiation hybrid lines. Proc Natl Acad Sci USA 101:9921–9926PubMedCrossRefGoogle Scholar
  21. Kynast RG, Okagaki RJ, Galatowitsch MW, Huettl PA, Jacobs MS, Stec AO, Walch MD, Cabral CB, Odland WE, Rines HW, Phillips RL (2005) Development of the first fertile plants with an addition of the whole maize chromosome 10 to the oat genome. Maize Genet Coop Newsl 79:38–39Google Scholar
  22. Kynast RG, Okagaki RJ, Granath SR, Rines HW, Phillips RL (2003) A novel maize ditelosomic addition to oat cv. Sun II for use in radiation hybrid mapping. Maize Genet Coop Newsl 77:62–63Google Scholar
  23. Kynast RG, Okagaki RG, Odland WE, Russell CD, Livingston SM, Rines HW, Phillips RL (2000) Towards a radiation hybrid map for maize chromosomes. Maize Genet Coop Newsl 74:60–61Google Scholar
  24. Kynast RG, Okagaki RJ, Odland WE, Stec A, Russell CD, Zaia H, Livingston SM, Rines HW, Phillips RL (2001) Oat-maize chromosome manipulation for the physical mapping of maize sequences. Maize Genet Coop Newsl 75:54–55Google Scholar
  25. Kynast RG, Okagaki RJ, Rines HW, Phillips RL (2002) Maize individualized chromosomes and derived radiation hybrid lines and their use in functional genomics. Funct Integr Genomics 2:60–69PubMedCrossRefGoogle Scholar
  26. Kynast RG, Riera-Lizarazu O, Vales MI, Okagaki RJ, Maquieira S, Chen G, Ananiev E V, Odland WE, Russell CD, Stec AO, Livingston SM, Zaia HA, Rines HW, Phillips RL (2001) A complete set of maize individual chromosome additions to the oat genome. Plant Physiol 125:1216–1227PubMedCrossRefGoogle Scholar
  27. Laurie DA, Bennett MD (1986) Wheat x maize hybridization. Can J Genet Cytol 28:313-316Google Scholar
  28. Laurie DA, Bennett MD (1989) The timing of chromosome elimination in hexaploid wheat x maize crosses. Genome 32:953–961Google Scholar
  29. Lee M, Phillips RL (1987a) Genetic variants in progeny of regenerated maize plants. Genome 29:834–838Google Scholar
  30. Lee M, Phillips RL (1987b) Genomic rearrangements in maize induced by tissue culture. Genome 29:122–128Google Scholar
  31. Li LJ, Arumuganathan K, Rines HW, Phillips RL, Riera-Lizarazu O, Sandhu D, Zhou Y, Gill KS (2001) Flow cytometric sorting of maize chromosome 9 from an oat-maize chromosome addition line. Theor Appl Genet 102:658–663CrossRefGoogle Scholar
  32. Mica E, Gianfranceschi L, Pe ME (2006) Characterization of five microRNA families in maize.J Exp Bot 57:2601–2612PubMedCrossRefGoogle Scholar
  33. Muehlbauer GJ, Riera-Lizarazu O, Kynast RG, Martin D, Phillips RL, Rines HW (2000) A maizechromosome 3 addition line of oat exhibits expression of the maize homeobox gene liguleless 3 and alterations of cell fate. Genome 43:1055–1064PubMedCrossRefGoogle Scholar
  34. Odland W, Baumgartner A, Phillips R (2006) Ancestral rice blocks define multiple related regions in the maize genome. The Plant Genome 1:S41–S48Google Scholar
  35. Okagaki, RJ, Jacobs MS, Stec AO, Kynast RG, Buescher E, Rines HW, Vales MI, Riera-Lizarazu O, Schneerman M, Doyle G, Friedman KL, Staub RW, Weber DF, Kamps TL, Amarillo IFE, Chase CD, Bass HW, Phillips RL (2008) Maize centromere mapping: A comparison of physical and genetic strategies. J Heredity 95:85–93CrossRefGoogle Scholar
  36. Okagaki RJ, Kynast RG, Livingston SM, Russell CD, Rines HW, Phillips, RL (2001) Mapping maize sequences to chromosome using oat-maize chromosome addition materials. Plant Physiol 125:1228–1235PubMedCrossRefGoogle Scholar
  37. Okagaki RJ, Kynast RG, Odland WE, Stec A, Russell CD, Zaia HA, Rines HW, Phillips RL (2002) A radiation hybrid system for the genetic and physical mapping of the corn genome. Maize Genet Coop Newsl 76:88–89Google Scholar
  38. Okagaki, R.J., Kynast, R.G., Rines, H.W. and Phillips RL (2004) Radiation hybrid mapping. In: Goodman RM (ed) Encyclopedia of plant & crop science. Marcel Dekker Inc, New York, pp 1074–1077Google Scholar
  39. Okagaki RJ, Jacobs M, Stec AO, Kynast RG, Zaia H, Granath SR, Rines HW, Phillips RL (2004) The distribution of chromosome breaks in radiation hybrid lines from chromosome 3. Maize Genet Coop Newsl 78:49–50Google Scholar
  40. Okagaki RJ, Schmidt CM, Stec AO, Rines HW, Phillips RL (2006) How many maize genes will not be in maize inbred B73? Maize Genet Coop Newsl 80:26Google Scholar
  41. Phillips RL, Rines HW, Okagaki RJ, Kynast RG, Donahue R, Odland WE (2003) Genetic and physical mapping of the maize genome through radiation hybrids. Proc Intl Rice Research Conference 2003 Gurdev Khush Symposium. In: Mew TW, Brar DS, Peng S, Dawe D, Hardy B (eds) Rice Science: Innovations and Impact for Livelihood, pp 77–90Google Scholar
  42. Riera-Lizarazu O, Rines HW, Phillips RL (1996) Cytological and molecular characterization of oat x maize partial hybrids. Theor Appl Genet 93:123–135CrossRefGoogle Scholar
  43. Riera-Lizarazu O, Vales MI, Ananiev EV, Rines HW, Phillips RL (2000) Production and characterization of maize-chromosome 9 radiation hybrids derived from an oat-maize chromosome addition line. Genetics 156:327–339PubMedGoogle Scholar
  44. Rines HW, Dahleen LS (1990) Haploid oat plants produced by application of maize pollen to emasculated oat florets. Crop Sci 30:1073–1078CrossRefGoogle Scholar
  45. Rines HW, Phillips RL, Kynast RG, Okagaki RJ, Odland WE, Stec AO, Jacobs MS, Granath SR. (2005) Maize chromosome additions and radiation hybrids and their use in dissecting the maize genome. In: Tuberosa R, Phillips RL, Gale M (eds) In the wake of the double helix: From the green revolution to the gene revolution. Avenue Media, Bologna, Italy pp 427–441Google Scholar
  46. Rines HW, Riera-Lizarazu O, Nunez VM, Davis DW and Phillips RL (1997) Oat haploids from anther culture and from wide hybridizations. In: SM Jain, K Sopory, RE Veilleux (eds) In Vitro Haploid Production in Higher Plants. Vol 4 Cereals Kluwer Academic Publ, Amsterdam, The Netherlands, pp 205–221Google Scholar
  47. Rines HW, Riera-Lizarazu O, Phillips RL (1995) Disomic maize chromosome-addition oat plants derived from oat x maize crosses. In: Oono K, Takaiwa F (eds) Modification of Gene Expression and Non-Mendelian Inheritance. Natl Inst Agrobiol Res Tsukuba, Japan, pp 235–251Google Scholar
  48. Vales MV, Riera-Lizarazu O, Rines HW, Phillips RL (2004) Transmission of maize chromosome 9 rearrangements in oat-maize radiation hybrids. Genome 47:1202–1210PubMedCrossRefGoogle Scholar
  49. Walch MD (2007) Expression of maize pathogenesis-related and photosynthetic genes in oat x maize addition lines. University of Minnesota MS thesisGoogle Scholar
  50. Zhang J, Simmons C, Yalpani N, Crane V, Wilkinson H, Kolomiets M (2006) Genomic analysis of the 12-oxo-phytodienoic acid reductase gene family of Zea mays. Plant Mol Biol 59:323–343CrossRefGoogle Scholar

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© Springer Science + Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Agronomy and Plant Genetics and Microbial and Plant Genomics InstituteUniversity of MinnesotaUSA

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