In Vitro and In Vivo Evaluation of Thin Calcium Phosphate Coatings

  • Ulrich M. Gross
  • Dirk Lassner


This chapter starts with general considerations on wound healing; the main cells and their structures and functions; and methods of in vitro and in vivo evaluation. Implants with thin calcium phosphate (CaP) coatings can enhance or inhibit wound healing mechanisms on various levels of gene expression and protein production. Therefore, some techniques to elucidate the material and tissue interactions are mentioned. Bone development, the various types of bone, and bone fracture healing are presented. Thin CaP coatings of implant material can be investigated in in vitro test systems using primary cells or cell lines in culture. More reliable results can be expected from in vivo investigations with animal models. Important results of such investigations are presented. The prediction of the clinical performance of thin CaP coatings on the basis of in vitro and in vivo models is limited. Therefore, retrieval studies of surgically explanted or postmortem uncovered implants should be obligatory.


Calcium Phosphate Bioactive Glass Biphasic Calcium Phosphate Amorphous Calcium Phosphate Bone Apposition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Plasmapore®-coated Ti6Al4V cylinders were supplied by Aesculap AG, Tuttlingen, Germany. The organoapatite coating of these cylinders was provided by J.J. Hwang and S.I. Stupp, Northwestern University, Evanston, IL, USA. The experimentation with coated and uncoated cylinders was performed by L. Pröbster, (Tübingen) and Konstanze I. Grun (Berlin).


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Institute of PathologyFree University of BerlinCampus Benjamin FranklinGermany
  2. 2.Institute of Cardiac Diagnostics and Therapy (IKDT)Germany

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