Integrated Plastic Microfluidic Devices for Bacterial Detection
This chapter describes integrated plastic microfluidic devices designed and fabricated for bacterial detection and identification. The devices, made from poly(cyclic olefin), contain components for DNA amplification, microfluidic valving, sample injection, on-column labeling and separation. DNA amplification was conducted in a reactor containing a volume ranging from 29 nL to 570 nL; screen-printed graphite ink resistors were used as heaters for thermal cycling. Microfluidic valves were created using in-situ gel polymerization to isolate the DNA amplification region from other components. Electrokinetic injection was used to migrate the amplification products (amplicons) through the gel valves, followed by on-chip electrophoretic separation. On-column labeling of the amplicons was achieved by mixing them with an intercalating dye. The various device functions were demonstrated by detection and genetic identification of two model bacteria, Escherichia coli O157 and Salmonella typhimurium.
KeywordsMicrofluidic Device Defense Advance Research Project Agency Cyclic Olefin Bacterial Detection Plastic Device
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- 14.Manz A, Harrison DJ, Verpoorte E et al. Planar Chips Technology for Miniaturization of Separation Systems—a Developing Perspective in Chemical Monitoring. Advances in Chromatography 1993; 33:1–66.Google Scholar
- 48.Anderson RC, Bogdan GJ, Puski A et al. Genetic Analysis Systems: Improvements and Methods. Solid-State Sensor and Actuator Workshop. Hilton Head Island, SC: Transducer Research Foundation, 1998:7–10.Google Scholar
- 54.Zhao M, Ricco AJ, Nguyen U et al. Functional and Efficient Electrode-integrated Microfluidic Plastic Devices. In: Ramsey JM, van den Berg A, eds. Micro Total Analysis Systems. Netherland: Kluwer Academic Publishers, 2001:193–194.Google Scholar