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Integrated Plastic Microfluidic Devices for Bacterial Detection

  • Z. Hugh Fan
  • Antonio J. Ricco
Part of the Biotechnology Intelligence Unit book series (BIOIU)

Abstract

This chapter describes integrated plastic microfluidic devices designed and fabricated for bacterial detection and identification. The devices, made from poly(cyclic olefin), contain components for DNA amplification, microfluidic valving, sample injection, on-column labeling and separation. DNA amplification was conducted in a reactor containing a volume ranging from 29 nL to 570 nL; screen-printed graphite ink resistors were used as heaters for thermal cycling. Microfluidic valves were created using in-situ gel polymerization to isolate the DNA amplification region from other components. Electrokinetic injection was used to migrate the amplification products (amplicons) through the gel valves, followed by on-chip electrophoretic separation. On-column labeling of the amplicons was achieved by mixing them with an intercalating dye. The various device functions were demonstrated by detection and genetic identification of two model bacteria, Escherichia coli O157 and Salmonella typhimurium.

Keywords

Microfluidic Device Defense Advance Research Project Agency Cyclic Olefin Bacterial Detection Plastic Device 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  1. 1.Department of Mechanical and Aerospace Engineering and Department of Biomedical EngineeringUniversity of FloridaGainesvilleUSA
  2. 2.National Center for Space Biological TechnologiesStanford UniversityStanfordUSA

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