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Nanochannels for Genomic DNA Analysis: The Long and the Short of It

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Integrated Biochips for DNA Analysis

Part of the book series: Biotechnology Intelligence Unit ((BIOIU))

Abstract

This review will discuss the theory of confined polymers in nanochannels and present our experiments, which test the theory and explore use of nanochannels for genomic analysis. Genomic length DNA molecules contained in nanochannels, which are less than one persistence length in diameter, are highly elongated. Thus, nanochannels can be used to analyze genomic length DNA molecules with very high linear spatial resolution. Also, nanochannels can be used to study the position and dynamics of proteins such as transcription factors that bind to DNA with high specificity. In order to realize these goals not only must nanochannels be constructed whose radius is less than the persistence length of DNA, but it is also necessary to understand the dynamics of polymers within nanochannels and develop experimental tools to study the dynamics of polymers in such confined volumes, tools which we review here.

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Authors and Affiliations

  1. Department of Physics, North Carolina State University, Raleigh, North Carolina, USA

    Robert Riehn

  2. Department of Physics, Princeton University, Princeton, New Jersey, USA

    Walter Reisner, Yan Mei Wang, Chih-Kuan Tung & Shuang-Fang Lim

  3. Lund University, Lund, Sweden

    Jonas O. Tegenfeldt

  4. Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA

    Edward Cox

  5. Department of Electrical Engineering, Princeton University, Princeton, New Jersey, USA

    James C. Sturm, Keith Morton, Steven Y. Chou & Robert H. Austin

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  1. Robert Riehn
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  2. Walter Reisner
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  3. Jonas O. Tegenfeldt
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  4. Yan Mei Wang
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  5. Chih-Kuan Tung
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  6. Shuang-Fang Lim
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  7. Edward Cox
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  8. James C. Sturm
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  9. Keith Morton
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  10. Steven Y. Chou
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  11. Robert H. Austin
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Correspondence to Robert Riehn .

Editor information

Editors and Affiliations

  1. Osmetech Molecular Diagnostics, Pasadena, California, USA

    Robin Hui Liu Ph.D.

  2. University of California at Irvine, Irvine, California, USA

    Abraham P. Lee Ph.D.

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Riehn, R. et al. (2007). Nanochannels for Genomic DNA Analysis: The Long and the Short of It. In: Liu, R.H., Lee, A.P. (eds) Integrated Biochips for DNA Analysis. Biotechnology Intelligence Unit. Springer, New York, NY. https://doi.org/10.1007/978-0-387-76759-8_12

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