Current Approaches for Engineering Proteins with Diverse Biological Properties

  • Isaac T. S. Li
  • Elizabeth Pham
  • Kevin Truong
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 620)


In the past two decades, protein engineering has advanced significantly with the emergence of new chemical and, genetic approaches. Modification and recombination of existing proteins not only produced novel enzymes used commercially and in research laboratories, but furthermore, they revealed the mechanisms of protein function. In this chapter, we will describe, the applications and significance of current protein engineering approaches.


Fluorescence Resonance Energy Transfer Direct Evolution Yellow Fluorescent Protein Random Mutagenesis Maltose Binding Protein 


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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  • Isaac T. S. Li
    • 1
    • 2
  • Elizabeth Pham
    • 1
    • 2
  • Kevin Truong
    • 1
    • 2
  1. 1.Institute of Biomaterials and Biomedical EngineeringUniversity of TorontoTorontoCanada
  2. 2.Edward S. Rogers Sr. Department of Electrical and Computer EngineeringUniversity of TorontoTorontoCanada

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