Fluorescent Nanoparticle for Bacteria and DNA Detection

  • Wenjun Zhao
  • Lin Wang
  • Weihong Tan
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 620)


Using bioconjugated dye-doped silica nanoparticles (NPs), we have developed a bioassay for the accurate determination of a single bacterial cell within 20 minutes without any signal amplification or sample enrichment. The antibody-conjugated NPs can specifically and quantitatively detect bacteria, such as Escherichia coli O157∶H7 from beef through antibody-antigen recognition. Dye-doped silica NPs have also been successfully used for DNA detection at sub-fentomolar concentrations. Our results demonstrate the potential of dye-doped silica NPs for broad applications in practical biotechnological and medical applications in various biodetection systems. The ultimate goal of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents.


Silica Nanoparticles Silica Sphere Single Bacterium Bacterium Detection Colloidal Silica Particle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  • Wenjun Zhao
    • 1
  • Lin Wang
    • 1
  • Weihong Tan
    • 1
  1. 1.Center for Research at The Bio/Nano Interface Department of Chemistry, Shands Cancer Center and UF Genetics InstituteUniversity of FloridaGainesvilleUSA

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