Abstract
Using bioconjugated dye-doped silica nanoparticles (NPs), we have developed a bioassay for the accurate determination of a single bacterial cell within 20 minutes without any signal amplification or sample enrichment. The antibody-conjugated NPs can specifically and quantitatively detect bacteria, such as Escherichia coli O157∶H7 from beef through antibody-antigen recognition. Dye-doped silica NPs have also been successfully used for DNA detection at sub-fentomolar concentrations. Our results demonstrate the potential of dye-doped silica NPs for broad applications in practical biotechnological and medical applications in various biodetection systems. The ultimate goal of integrating bionanotechnology into complex biological systems will emerge as a revolutionary tool for ultrasensitive detection of disease markers and infectious agents.
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© 2007 Landes Bioscience and Springer Science+Business Media
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Zhao, W., Wang, L., Tan, W. (2007). Fluorescent Nanoparticle for Bacteria and DNA Detection. In: Chan, W.C.W. (eds) Bio-Applications of Nanoparticles. Advances in Experimental Medicine and Biology, vol 620. Springer, New York, NY. https://doi.org/10.1007/978-0-387-76713-0_10
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DOI: https://doi.org/10.1007/978-0-387-76713-0_10
Publisher Name: Springer, New York, NY
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