Antibody Radiolabeling

  • Corinne Bensimon
  • Russell Redshaw
Part of the Biotechnology: Pharmaceutical Aspects book series (PHARMASP, volume XI)


The concept of using radiolabeled antibodies in vivo dates back to 1949 when Pressman (1949) demonstrated that radiolabeled antibodies could be used to localize specific tissue. Using 131I radiolabeled anti-carcinoembryonic antigen antibodies, Goldenberg et al. (1978) imaged human colonic carcinoma xenografts and in doing so, set in motion the field of radiolabeled antibodies for use in imaging and therapy.

Antibody based imaging entails either an antibody, bivalent fragment F(ab′)2, monovalent fragment Fab′ or antibody construct (minibody, diabody, tribody, etc.), with either a gamma or positron emitting radioisotope, having an appropriate energy, and an external camera system. A list of radioisotopes suitable for imaging is shown in Table 19-1.


Instant Thin Layer Chromatography Ibritumomab Tiuxetan Bifunctional Chelate Antibody Radiolabeling Central Manufacturing 


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Copyright information

© American Association of Pharmaceutical Scientists 2010

Authors and Affiliations

  • Corinne Bensimon
    • 1
  • Russell Redshaw
    • 2
  1. 1.Canadian Molecular Imaging Center of Excellence (CMICE), MDS Nordion, University of Ottawa Heart InstituteOttawaCanada
  2. 2.Science and Technology Partnerships, MDS NordionOttawaCanada

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