Precise Measurements of Diffusion in Solution by Fluorescence Correlations Spectroscopy

  • Jörg Enderlein


The chapter presents a general introduction to the method of fluorescence correlation spectroscopy (FCS) and its recently developed modification, dual-focus FCS. It explains the general optical setup of an FCS system, data acquisition, and data analysis. It discusses numerous potential optical and photophysical artifacts of FCS measurements and explains how these problems are circumvented by dual-focus FCS.


Apparent Diffusion Coefficient Fluorescence Correlation Spectroscopy Dichroic Mirror Detection Volume Fluorescence Photon 
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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jörg Enderlein
    • 1
  1. 1.Drittes Physikalisches Institut, Georg-August-Universität GöttingenGöttingenGermany

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