Single-Molecule Enzymology

  • Joseph J. Loparo
  • Antoine van Oijen


Understanding how enzymes function requires a thorough characterization of enzymatic dynamics. Traditional enzymatic assays average over an ensemble of molecules, making it difficult to detect reaction intermediates and conformational fluctuations of the enzyme. These problems can be overcome by observing enzymes functioning in real time on the single-molecule level. This chapter describes recent research efforts to measure single-molecule enzyme kinetics and observe the structural dynamics of enzymes and discusses new approaches to study multiprotein complexes on the single-molecule level.


Fluorescence Resonance Energy Transfer Fluorescence Lifetime Flavin Adenine Dinucleotide Catalytic Rate Total Internal Reflection Fluorescence 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Joseph J. Loparo
    • 1
  • Antoine van Oijen
    • 1
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA

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