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Recognition Imaging Using Atomic Force Microscopy

  • Andreas Ebner
  • Lilia Chtcheglova
  • Jilin Tang
  • David Alsteens
  • Vincent Dupres
  • Yves F. Dufrêne
  • Peter Hinterdorfer
Chapter

Abstract

Recognition imaging using atomic force microscopy (AFM) offers a wealth of new opportunities in biophysical research, such as its ability to localize specific chemical groups and biological receptors on biosurfaces and to measure their molecular-scale interactions. By attaching well-defined chemical groups on tips, it is possible to map chemical properties and interactions on cell surfaces on a scale of a few functional groups. Single-molecule force spectroscopy with tips functionalized with relevant bioligands provides a means of localizing individual receptors and measuring their specific binding forces. Alternatively, recognition sites may also be mapped with unprecedented temporal resolution using dynamic recognition imaging, in which molecular recognition signals are detected during dynamic force microscopy imaging. These AFM modalities, which all have functionalization of the tips with specific molecules in common, provide new avenues for understanding the structure–function of cell surfaces in connection with medical and physiological issues.

Keywords

Atomic Force Microscopy Adhesion Force Recognition Image Cystic Fibrosis Transmembrane Regulator Force Spectroscopy 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This work was supported by the National Foundation for Scientific Research (FNRS), the Université Catholique de Louvain (Fonds Spéciaux de Recherche), the Région Wallonne, the Federal Office for Scientific, Technical and Cultural Affairs (Interuniversity Poles of Attraction Programme), and the Research Department of the Communauté Française de Belgique (Concerted Research Action). YD and DA are Research Associate and Research Fellow of the FRS-FNRS, respectively. This work was further supported by the FFG program of the European Union projects Tips4Cells (LSHG-CT2005-512101), NASSAP (EC-STREP-13532), BioLightTouch (028181), and IMMUNANOMAP (MRTN-CT-2006-035946). AE and LC are Research Associates of the BioLigthTouch, and JT is Research Associate of the NASSAP project.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Andreas Ebner
    • 1
  • Lilia Chtcheglova
    • 1
  • Jilin Tang
    • 1
  • David Alsteens
    • 2
  • Vincent Dupres
    • 2
  • Yves F. Dufrêne
    • 2
  • Peter Hinterdorfer
    • 1
  1. 1.Institute for Biophysics, Johannes Kepler University of LinzA-4040 LinzAustria
  2. 2.Unité de chimie des interfaces, Université catholique de LouvainB-1348 Louvain-la-NeuveBelgium

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