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Taurine 7 pp 491-499 | Cite as

Effect of Taurine on Antioxidant Enzyme System in B16F10 Melanoma Cells

  • Jisun Yu
  • An Keun Kim
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 643)

abstract

There is now increasing evidence that free radicals and reactive oxygen species (ROS) are involved in a variety of pathological events. Reactive oxygen species are produced during normal cellular function and lead to lipid peroxidation, massive protein oxidation and degradation. Taurine is an abundant free amino acid in inflammatory cells, where it is thought to be cytoprotective. The aim of the present study was to examine whether taurine enhances endogenous antioxidant enzyme activity and/ or regulates ROS generation in B16F10 mouse melanoma cells. B16F10 cells were exposed to medium containing taurine for a period of 24 h. Cell viability, measured by the MTT assay, exhibited a dose-dose dependent inhibition. Taurine increased the activities of superoxide dismutase, glutathione peroxidase and CAT compared to those of the control, an effect paralleling an increase in gene expression. Taurine also reduced ROS content in a dose-dependent manner. Taken together, our results suggest that taurine decreases ROS levels by increasing the levels of the antioxidant enzymes.

Keywords

Reactive Oxygen Species Antioxidant Enzyme Melanoma Cell Reactive Oxygen Species Level B16F10 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jisun Yu
    • 1
  • An Keun Kim
  1. 1.College PharmacySookmyung Women’s UniversitySeoulKorea

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