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Taurine 7 pp 481-489 | Cite as

Inhibition of Apoptosis by Taurine in Macrophages Treated with Sodium Nitroprusside

  • So Young Kim
  • Taesun Park
  • Ha Won Kim
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 643)

abstract

Nitric oxide (NO) induces apoptotic cell death in murine RAW264.7 macrophages. To elucidate the mechanism underlying the inhibitory effect of taurine on NO-induced apoptosis, a cell was exposed to sodium nitroprusside (SNP), an NO donor, in the absence and presence of taurine. Taurine treatment prevented SNP-mediated cellular apoptosis in a concentration dependent manner. The exposure of the cell to taurine prior to SNP treatment inhibited DNA fragmentation more than addition of taurine to the medium after SNP treatment. Agarose gel electrophoresis data revealed that taurine reduced the intensity of SNP-induced DNA laddering. The taurine-mediated reduction in the number of apoptotic cells was also observed using the Hoechst 33258 stain. These results support the idea that taurine has the potential to function as an inhibitory modulator of NO-mediated cell injury.

Keywords

Nitric Oxide Nitric Oxide Sodium Nitroprusside Human Vascular Endothelial Cell Murine Macrophage Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • So Young Kim
    • 1
  • Taesun Park
  • Ha Won Kim
  1. 1.College of MedicineEwha Women’s UniversitySeoulKorea

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