Taurine 7 pp 451-461 | Cite as

Taurine Chloramine: A Possible Oxidant Reservoir

  • Tetsuya Ogino
  • Tin Aung Than
  • Mutsumi Hosako
  • Michitaka Ozaki
  • Masako Omori
  • Shigeru Okada
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 643)


Taurine is abundant in polymorphonuclear leukocytes (PMNs) where it reacts with PMN-derived hypochlorous acid to form taurine chloramine (Tau-NHCl), a substance that does not readily cross the cell membrane. When PMNs were stimulated in PBS lacking taurine, extracellular oxidant concentration was low, but the concentration increased 3–4 fold when 15 mM taurine was added, indicating that taurine lowers oxidant levels inside the cell. When Tau-NHCl was added to Jurkat cells in suspension, its half life was about 75 min. In contrast, membrane-permeable ammonia mono-chloramine NH2Cl) has a half life of only 6 min. Accordingly, NH2Cl oxidizes cytosolic proteins, such as I\upkappaB, and inhibits NF-κB activation, whereas Tau-NHCl exhibits no comparable effect. However, when NH4 + was added to the medium, Tau-NHCl oxidizes IκB and inhibits NF-κB activation, probably through oxidant transfer to NH4 + leading to NH2Cl formation. These results indicate that Tau-NHCl can serve as an oxidant reservoir, exhibiting either delayed oxidant effects or acting as an oxidant at a distant site.


Jurkat Cell Respiratory Burst Leukoc Biol Opsonized Zymosan Natural Killer Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Tetsuya Ogino
    • 1
  • Tin Aung Than
  • Mutsumi Hosako
  • Michitaka Ozaki
  • Masako Omori
  • Shigeru Okada
  1. 1.Pathology and Experimental MedicineOkayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesOkayamaJapan

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