Protective Effect of Taurine against Nitrosative Stress in the Stomach of Rat with Water Immersion Restraint Stress
In the present study, we examined by immunohistochemistry the formation and distribution of 8-nitroguanine, a sensitive marker of nitrosative DNA damage, in rat stomach of rats subjected to water immersion restraint stress (WIR). WIR induced an increase in 8-nitroguanine content of gastric gland epithelium. 8-Nitroguanine immunoreactivity, which was observed mainly in the nuclei of stomach epithelium, increased with the severity of inflammation. Expression of iNOS was also observed in the inflammatory cells of lamina propria. Therefore, it is logical that iNOS-mediated nitrosative stress must participate in the development of ulcers through apoptotic cell death linked to the formation of 8-nitroguanine during chronic inflammation. Taurine administration attenuated stress-induced gastric mucosal injury. These results demonstrate that nitrosative stress participates in stress-mediated ulcer formation. Taurine exerts a prophylactic effect against mucosal lesions of the stomach caused by stress. This effect of taurine may have a potential clinical benefit in preventing gastritis associated with stress.
KeywordsGastric Mucosa Parietal Cell Nitrosative Stress Gastric Gland Gastric Mucosal Injury
Unable to display preview. Download preview PDF.
- Crowther JE, Kutala VK, Kuppusamy P, Ferguson JS, Beharka AA, Zweier JL, McCormac FX, Schlesinger LS (2004) Pulmonary surfactant protein a inhibits macrophage reactive oxygen intermediate production in response to stimuli by reducing NADPH oxidase activity. J Immunol 172:6866–6874PubMedGoogle Scholar
- Hung CR (2006) Effect of taurine on gastric oxidative stress and hemorrhagic erosion in brain ischemic rats. Chinese J Physiol 49:152–159Google Scholar
- Liu Y, Tonna-Demasi M, Park E, Schuller-Levis G, Quinn MR (1998) Taurine chloramines inhibits production of nitric oxide and prostaglandin E2 in activated C6 glioma cells by suppressing inducible nitric oxide synthase and cyclooxygenase-2 expression. Molecular Brain Res 59: 189–195CrossRefGoogle Scholar
- Pinlaor S, Hiraku Y, Ma N, Yongvanit P, Semba R, Oikawa S, Murata M, Sripa B, Sithithaworn P, Kawanishi S (2004) Mechanism of NO-mediated oxidative and nitrative DNA damage in hamsters infected with Opisthorchis viverrini: a model of inflammation-mediated carcinogenesis. Nitric Oxide 11:175–183PubMedCrossRefGoogle Scholar