Taurine 7 pp 181-190 | Cite as

Taurine and Guanidinoethanesulfonic Acid (GES) Differentially Affects the Expression and Phosphorylation of Cellular Proteins in C6 Glial Cells

  • Hemanta K. Sarkar
  • Thanh T. Tran
  • Rao Papineni
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 643)


Effects of taurine and guanidinoethanesulfonic acid (GES), a taurine transport inhibitor, on the expression and phosphorylation of cellular proteins in C6 glial cells were examined using two-dimensional (2-D) gel electrophoresis and 2-D immunoblots. 2-D gels stained with Coomassie Blue or SYPRO Ruby showed differential distribution patterns of cellular proteins in the control, taurine-supplemented and GES-supplemented cells. 2-D immunoblot analysis using the anti-phosphotyrosine antibody recognized only few immuno-reactive proteins in all three samples, and their distribution patterns were different. On the other hand, 2-D immunoblot analysis using the anti-phosphothreonine antibody recognized many immuno-reactive proteins with distinctly different distribution patterns in the control, taurine-supplemented and GES-supplemented cells. In GES-supplemented cells, the relative number of the anti-phosphotyrosine immuno-reactive protein spots increased modestly, whereas the relative number of the anti-phosphothreonine immuno-reactive protein spots decreased markedly than those in the control and taurine-supplemented cells.


Phosphorylation Pattern Taurine Supplementation Bile Salt Export Pump Taurine Depletion Intracellular Taurine 
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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Hemanta K. Sarkar
    • 1
  • Thanh T. Tran
  • Rao Papineni
  1. 1.BBTech Inc.DartmouthUSA

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