Insertional Mutagenesis as a Tool to Study Genes/Functions in Chlamydomonas

  • Aurora Galván
  • David González-Ballester
  • Emilio Fernández
Part of the Advances in Experimental Medicine and Biology book series (volume 616)


The unicellular alga Chlamydomonas reinhardtii has emerged during the last decades as a model system to understand gene functions, many of them shared by bacteria, fungi, plants, animals and humans. A powerful resource for the research community is the availability of complete collections of stable mutants for studying whole genome function. In the meantime other strategies might be developed; insertional mutagenesis has become currently the best strategy to disrupt and tag nuclear genes in Chlamydomonas allowing forward and reverse genetic approaches. Here, we outline the mutagenesis technique stressing the idea of generating databases for ordered mutant libraries, and also of improving efficient methods for reverse genetics to identify mutants defective in a particular gene.


Insertional Mutagenesis Reverse Genetic Mutant Library Nitrate Assimilation Nuclear Transformation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Landes Bioscience and Springer Science+Business Media 2007

Authors and Affiliations

  • Aurora Galván
    • 1
    • 2
  • David González-Ballester
    • 2
    • 3
  • Emilio Fernández
    • 2
  1. 1.Departamento de Bioquímica y Biología Molecular, Facultad de CienciasUniversidad de CórdobaCórdobaSpain
  2. 2.Departamento de Bioquímica y Biología Molecular Campus de RabanalesUniversidad de CódobaCódobaSpain
  3. 3.Department of Plant Biology The Carnegie Institution of WashingtonUniversity of StanfordStanfordUSA

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