Phase Behavior of Phospholipid-Cholesterol Liposomes Stabilized With Trehalose

  • S. Ohtake
  • C. Schebor
  • J. J. de Pablo
Conference paper
Part of the Food Engineering series book series (FSES)

Achieving long-term stability in biological systems has been a long-standing goal of the food, pharmaceutical, and biomedical industries. Avoiding the need for refrigeration would reduce production and storage costs drastically. The desiccation of phospholipidic vesicles has been studied in an effort to understand biological membranes under low water content conditions (Crowe and Crowe, 1988; Ohtake et al., 2004).

Trehalose is effective in protecting biological membranes upon freeze-drying, and it has been widely used to preserve the integrity of phospholipid liposomes (Crowe and Crowe, 1988; Ohtake, et al., 2004; Crowe et al., 1986). Despite the abundance of cholesterol in mammalian plasma membranes (Rouser, et al., 1968), studies examining the effects of dehydration on cholesterol-containing model membranes are scarce (Van Winden and Crommelin, 1999; Harrigan, et al., 1990). Furthermore, the ability of well known lyoprotectants, such as trehalose, to stabilize cholesterol-containing liposomes has not been examined in detail. The aim of this work is to understand how cholesterol containing liposomes behave upon lyophilization.


Phase Behavior Phase Transition Temperature DPPC Liposome Phospholipid Liposome Mammalian Plasma Membrane 
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  1. Blume A., 1980, Thermotropic Behavior of Phosphatidylethanolamine-Cholesterol and Phosphatidylethanol-Amine-Phosphatidylcholine-Cholesterol Mixtures, Biochemistry 19:4908–4913.CrossRefGoogle Scholar
  2. Blume, A., and Ackermann T., 1974, Calorimetric Study of Lipid Phase-Transitions in Aqueous Dispersions of Phosphorylcholine-Phosphorylethanolamine Mixtures, FEBS Lett. 43:1–74.CrossRefGoogle Scholar
  3. Crowe, J., Carpenter, J., and Crowe L., 1998, The Role of Vitrification in Anhydrobiosis, Annu. Rev. Physiol. 6:73–103.CrossRefGoogle Scholar
  4. Crowe, L., and Crowe J., 1988, Trehalose and Dry Dipalmitoylphosphatidylcholine Revisited, Biochim. Biophys. Acta 946:193–201.CrossRefGoogle Scholar
  5. Crowe, L., Womersley, C., Crowe, J., Reid, D., Appel, L., and Rudolph A., 1986, Prevention of Fusion and Leakage in Freeze-Dried Liposomes by Carbohydrates, Biochim. Biophys. Acta 861:131–140.Google Scholar
  6. Handa, T. Ichihashi, C., and Nakagaki M., 1985, Polymorphic Phase Transition and Monomolecular Spreading of Synthetic Phospholipids, Prog. Colloid Polym. Sci. 71:26–31.CrossRefGoogle Scholar
  7. Harrigan, P., Madden, T., and Cullis P., 1990, Protection of Liposomes During Dehydration or Freezing, Chem. Phys. Lipids 52:139–149.CrossRefGoogle Scholar
  8. McMullen, T., Lewis, T., and McElhaney R., 1993, Differential Scanning Calorimetric Study of The Effect of Cholesterol on the Thermotropic Phase Behavior of a Homologous Series of Linear Saturated Phosphatidyl-Cholines, Biochemistry 32:16–522.CrossRefGoogle Scholar
  9. Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2004, Effect of Sugar-Phosphate Mixtures on the Stability of DPPC Membranes in Dehydrated Systems, Cryobiology 48:81–89.CrossRefGoogle Scholar
  10. Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2005, Phase Behavior of Freeze-Dried Phospholipids-Cholesterol Mixtures Stabilized with Trehalose, Biochim. Biophys. Acta 1713:57–64.CrossRefGoogle Scholar
  11. Rouser, G., Nelson, G., Fleischer, S., and Simon G., 1968, Biological Membranes Physical Fact and Function, D. Chapman-Academic Press, London, pp. 5–69.Google Scholar
  12. Tsvetkova, N., Philips, B., Crowe, L., Crowe, J., and Risbud S., 1998, Effect of Sugar on Headgroup Mobility in Freeze-Dried Dipalmitoylphosphatidylcholine Bilayers: Solid-State 31P NMR and FTIR Studies, Biophys. J. 75:2947–2955.CrossRefGoogle Scholar
  13. Van Winden, E., and Crommelin D., 1999, Short Term Stability of Freeze-Dried, Lyoprotected Liposomes, J. Controlled Release 58:69–86.CrossRefGoogle Scholar
  14. Vist, M., and Davis J., 1990, Phase Equilibria of Cholesterol/Dipalmitoylphosphatidylcholine Mixtures: 2H Nuclear Magnetic Resonance and Differential Scanning Calorimetry, Biochemistry 29:451–464.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • S. Ohtake
    • 1
  • C. Schebor
    • 2
  • J. J. de Pablo
    • 1
  1. 1.Department of Chemical and Biological EngineeringUniversity of WisconsinMadisonUSA
  2. 2.Departamento de Industrias y de Química OrgánicaUniversidad de Buenos AiresArgentina

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