Achieving long-term stability in biological systems has been a long-standing goal of the food, pharmaceutical, and biomedical industries. Avoiding the need for refrigeration would reduce production and storage costs drastically. The desiccation of phospholipidic vesicles has been studied in an effort to understand biological membranes under low water content conditions (Crowe and Crowe, 1988; Ohtake et al., 2004).
Trehalose is effective in protecting biological membranes upon freeze-drying, and it has been widely used to preserve the integrity of phospholipid liposomes (Crowe and Crowe, 1988; Ohtake, et al., 2004; Crowe et al., 1986). Despite the abundance of cholesterol in mammalian plasma membranes (Rouser, et al., 1968), studies examining the effects of dehydration on cholesterol-containing model membranes are scarce (Van Winden and Crommelin, 1999; Harrigan, et al., 1990). Furthermore, the ability of well known lyoprotectants, such as trehalose, to stabilize cholesterol-containing liposomes has not been examined in detail. The aim of this work is to understand how cholesterol containing liposomes behave upon lyophilization.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Blume A., 1980, Thermotropic Behavior of Phosphatidylethanolamine-Cholesterol and Phosphatidylethanol-Amine-Phosphatidylcholine-Cholesterol Mixtures, Biochemistry 19:4908–4913.
Blume, A., and Ackermann T., 1974, Calorimetric Study of Lipid Phase-Transitions in Aqueous Dispersions of Phosphorylcholine-Phosphorylethanolamine Mixtures, FEBS Lett. 43:1–74.
Crowe, J., Carpenter, J., and Crowe L., 1998, The Role of Vitrification in Anhydrobiosis, Annu. Rev. Physiol. 6:73–103.
Crowe, L., and Crowe J., 1988, Trehalose and Dry Dipalmitoylphosphatidylcholine Revisited, Biochim. Biophys. Acta 946:193–201.
Crowe, L., Womersley, C., Crowe, J., Reid, D., Appel, L., and Rudolph A., 1986, Prevention of Fusion and Leakage in Freeze-Dried Liposomes by Carbohydrates, Biochim. Biophys. Acta 861:131–140.
Handa, T. Ichihashi, C., and Nakagaki M., 1985, Polymorphic Phase Transition and Monomolecular Spreading of Synthetic Phospholipids, Prog. Colloid Polym. Sci. 71:26–31.
Harrigan, P., Madden, T., and Cullis P., 1990, Protection of Liposomes During Dehydration or Freezing, Chem. Phys. Lipids 52:139–149.
McMullen, T., Lewis, T., and McElhaney R., 1993, Differential Scanning Calorimetric Study of The Effect of Cholesterol on the Thermotropic Phase Behavior of a Homologous Series of Linear Saturated Phosphatidyl-Cholines, Biochemistry 32:16–522.
Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2004, Effect of Sugar-Phosphate Mixtures on the Stability of DPPC Membranes in Dehydrated Systems, Cryobiology 48:81–89.
Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2005, Phase Behavior of Freeze-Dried Phospholipids-Cholesterol Mixtures Stabilized with Trehalose, Biochim. Biophys. Acta 1713:57–64.
Rouser, G., Nelson, G., Fleischer, S., and Simon G., 1968, Biological Membranes Physical Fact and Function, D. Chapman-Academic Press, London, pp. 5–69.
Tsvetkova, N., Philips, B., Crowe, L., Crowe, J., and Risbud S., 1998, Effect of Sugar on Headgroup Mobility in Freeze-Dried Dipalmitoylphosphatidylcholine Bilayers: Solid-State 31P NMR and FTIR Studies, Biophys. J. 75:2947–2955.
Van Winden, E., and Crommelin D., 1999, Short Term Stability of Freeze-Dried, Lyoprotected Liposomes, J. Controlled Release 58:69–86.
Vist, M., and Davis J., 1990, Phase Equilibria of Cholesterol/Dipalmitoylphosphatidylcholine Mixtures: 2H Nuclear Magnetic Resonance and Differential Scanning Calorimetry, Biochemistry 29:451–464.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2008 Springer Science+Business Media, LLC
About this paper
Cite this paper
Ohtake, S., Schebor, C., de Pablo, J.J. (2008). Phase Behavior of Phospholipid-Cholesterol Liposomes Stabilized With Trehalose. In: Gutiérrez-López, G.F., Barbosa-Cánovas, G.V., Welti-Chanes, J., Parada-Arias, E. (eds) Food Engineering: Integrated Approaches. Food Engineering series. Springer, New York, NY. https://doi.org/10.1007/978-0-387-75430-7_28
Download citation
DOI: https://doi.org/10.1007/978-0-387-75430-7_28
Publisher Name: Springer, New York, NY
Print ISBN: 978-0-387-75429-1
Online ISBN: 978-0-387-75430-7
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)