Phase Behavior of Phospholipid-Cholesterol Liposomes Stabilized With Trehalose
Part of the
Food Engineering series
book series (FSES)
Achieving long-term stability in biological systems has been a long-standing goal of the food, pharmaceutical, and biomedical industries. Avoiding the need for refrigeration would reduce production and storage costs drastically. The desiccation of phospholipidic vesicles has been studied in an effort to understand biological membranes under low water content conditions (Crowe and Crowe, 1988; Ohtake et al., 2004).
Trehalose is effective in protecting biological membranes upon freeze-drying, and it has been widely used to preserve the integrity of phospholipid liposomes (Crowe and Crowe, 1988; Ohtake, et al., 2004; Crowe et al., 1986). Despite the abundance of cholesterol in mammalian plasma membranes (Rouser, et al., 1968), studies examining the effects of dehydration on cholesterol-containing model membranes are scarce (Van Winden and Crommelin, 1999; Harrigan, et al., 1990). Furthermore, the ability of well known lyoprotectants, such as trehalose, to stabilize cholesterol-containing liposomes has not been examined in detail. The aim of this work is to understand how cholesterol containing liposomes behave upon lyophilization.
KeywordsPhase Behavior Phase Transition Temperature DPPC Liposome Phospholipid Liposome Mammalian Plasma Membrane
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Blume A., 1980, Thermotropic Behavior of Phosphatidylethanolamine-Cholesterol and Phosphatidylethanol-Amine-Phosphatidylcholine-Cholesterol Mixtures, Biochemistry
Blume, A., and Ackermann T., 1974, Calorimetric Study of Lipid Phase-Transitions in Aqueous Dispersions of Phosphorylcholine-Phosphorylethanolamine Mixtures, FEBS Lett
Crowe, J., Carpenter, J., and Crowe L., 1998, The Role of Vitrification in Anhydrobiosis, Annu. Rev. Physiol
Crowe, L., and Crowe J., 1988, Trehalose and Dry Dipalmitoylphosphatidylcholine Revisited, Biochim. Biophys. Acta
Crowe, L., Womersley, C., Crowe, J., Reid, D., Appel, L., and Rudolph A., 1986, Prevention of Fusion and Leakage in Freeze-Dried Liposomes by Carbohydrates, Biochim. Biophys. Acta
Handa, T. Ichihashi, C., and Nakagaki M., 1985, Polymorphic Phase Transition and Monomolecular Spreading of Synthetic Phospholipids, Prog. Colloid Polym. Sci
Harrigan, P., Madden, T., and Cullis P., 1990, Protection of Liposomes During Dehydration or Freezing, Chem. Phys. Lipids
McMullen, T., Lewis, T., and McElhaney R., 1993, Differential Scanning Calorimetric Study of The Effect of Cholesterol on the Thermotropic Phase Behavior of a Homologous Series of Linear Saturated Phosphatidyl-Cholines, Biochemistry
Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2004, Effect of Sugar-Phosphate Mixtures on the Stability of DPPC Membranes in Dehydrated Systems, Cryobiology
Ohtake, S., Schebor, C., Palecek, S., and de Pablo J.J., 2005, Phase Behavior of Freeze-Dried Phospholipids-Cholesterol Mixtures Stabilized with Trehalose, Biochim. Biophys. Acta
Rouser, G., Nelson, G., Fleischer, S., and Simon G., 1968, Biological Membranes Physical Fact and Function
, D. Chapman-Academic Press, London, pp. 5–69.Google Scholar
Tsvetkova, N., Philips, B., Crowe, L., Crowe, J., and Risbud S., 1998, Effect of Sugar on Headgroup Mobility in Freeze-Dried Dipalmitoylphosphatidylcholine Bilayers: Solid-State 31P NMR and FTIR Studies, Biophys. J
Van Winden, E., and Crommelin D., 1999, Short Term Stability of Freeze-Dried, Lyoprotected Liposomes, J. Controlled Release
Vist, M., and Davis J., 1990, Phase Equilibria of Cholesterol/Dipalmitoylphosphatidylcholine Mixtures: 2H Nuclear Magnetic Resonance and Differential Scanning Calorimetry, Biochemistry
© Springer Science+Business Media, LLC 2008