Predictive Equations to Assess the Effect of Lactic Acid and Temperature on Bacterial Growth in a Model Meat System

  • F. Coll Cárdenas
  • L. Giannuzzi
  • N. E. Zaritzky
Conference paper
Part of the Food Engineering series book series (FSES)

Meat microflora is mainly composed of Acinetobacter, Moraxella, Brochothrix termosphacta, Lactobacillus, Pseudomonas and Enterobacteriaceae family genera, such as Klebsiella sp. and E. coli. In natural conditions meat pH can range from about 6.0 (being close to the optimum level for most pathogenic and alteration-causing bacteria) to values close to 5.5, at which microbial growth rate decreases significantly. Combining low pH with other factors such as low temperatures can almost completely prevent microbial growth from occurring. Muscle pH variation is highly dependent on the tissue glycogen level at the time of slaughter.

Weak organic acids tend to be more effective as antimicrobials than strong acids because they acidify the interior of the cell (Anderson et al., 1987). Antimicrobial activities exerted by organic acids depend upon i) pH reduction, ii) minimizing dissociation of the acid and iii) maximizing toxicity of the acid molecule (Anderson et al., 1987). Lactic acid produces an inhibitory effect because of the decrease in pH; this acid could act both on the meat muscle flora itself and on that of the fat, although such antimicrobial effect varies, not only according to the type of acid used, but also according to the microbial variety to be treated. Sometimes it could be bacteriostatic and sometimes it could have a bactericidal action. High efficiency in meat surface sanitization due to lactic acid addition has been widely reported (Nakai and Siebert, 2004).

The objective of this work is to analyze and mathematically model the effect of storage temperatures (0°C, 4°C and 10°C) on the growth of three microorganisms isolated from beef samples: Klebsiella sp., E. coli and Pseudomonas sp., inoculated in a culture broth with different concentrations of lactic acid leading to pH values ranging between 5.6 and 6.1.


Lactic Acid Specific Growth Rate Predictive Equation Microbial Count Lactic Acid Concentration 
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  1. Anderson, G.R., Vanderzant, C., Savell, J.W., Jones, D.R., Griffin, D.K., and Ehlers J.D., 1987, Effect of Decontamination of Beef Subprimal Cuts on the Microbiological and Sensory Characteristics of Steaks. Meat Science 19:217–226.CrossRefGoogle Scholar
  2. Andrés, S., Giannuzzi, L., and Zaritzky N.E., 2001, Mathematical Modelling of Microbial Growth in Packaged Refrigerated Orange Juice Treated with Chemical Preservatives, J. Food Sci. 66:724–728.CrossRefGoogle Scholar
  3. AOAC, 1984, Official Methods of Analysis. 14th ed. Assoc. Official Agric. Chemists. Washington, D.C.Google Scholar
  4. Cudjoe, K., 1988, The Effect of Lactic Acid Sprays on the Keeping Qualities of Meat During Storage, Int. J. Food Microbiol. 7:1–7.CrossRefGoogle Scholar
  5. Giannuzzi, L., Pinotti, A., and Zaritzky N., 1998, Mathematical Modelling of Microbial Growth in Packaged Refrigerated Beef Stored at Different Temperatures, Int. J. Food Microbiol. 39:101–110.CrossRefGoogle Scholar
  6. Gibson, A.M., Bratchell, N., and Roberts, T.A., 1988, The Effect of Sodium Chloride and Temperature on Rate and Extent of Growth of Clostridium botulinum Type A in Pasteurized Pork Slurry, J. Appl. Bacteriol. 62:479–490.Google Scholar
  7. Gill, C., and Newton K., 1982, Effect of Lactic Acid Concentration on Growth on Meat of Gram–Negative Psychrotrophs from a Meatworks, Appl. Environ. Microbiol. 43:284–288.Google Scholar
  8. Gill, C.O., and Badoni M., 2004, Effects of Peroxyacetic Acid, Acidified Sodium Chlorite or Lactic Acid Solutions on the Microflora of Chilled Beef Carcasses, Int. J. Food Microbiol. 91:43–50.CrossRefGoogle Scholar
  9. MacFaddin, J.F., 1979, Biochemical Test for Identification of Medical Bacteria. Baltimore: The Williams & Wilkins Company.Google Scholar
  10. Masurovsky, E.B., Goldblith, S.A., and Voss J., 1965, Differential Medium for Selection and Enumeration of Members of the Pseudomonas, J. Bacteriol. 85: 722.Google Scholar
  11. Nakai, S.A., and Siebert K.J., 2004, Organic Acid Inhibition Models for Listeria innocua, Listeria ivanovii, Pseudomonas aeruginosa and Oenococcus oeni, Food Microbiol. 21(1):67–72.CrossRefGoogle Scholar
  12. Snijders, J.M.A., van Logtestijn, T.G., Mossel, D.A.A., and Smulders F.T.M., 1984, Conditions for the Use of Lactic Acid as a Decontaminant in the Meat Industry, Proc. Eur. Meet. Meat Res. Work 30:232–233.Google Scholar
  13. Whiting, R., 1995, Microbial Modeling in Foods, Food Sci. Nutr. 35:467–494.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • F. Coll Cárdenas
    • 1
  • L. Giannuzzi
    • 1
  • N. E. Zaritzky
    • 1
  1. 1.Centro de Investigación y Desarrollo en Criotecnología de AlimentosUniversidad NacionalArgentina

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