Production of Spores

  • Sumitra Ramachandran
  • Christian Larroche
  • Ashok Pandey


Spores are generally defined as small reproductive bodies that get detached from the microbial species to produce a new offspring. In the process they do not fuse with other reproductive bodies, but get germinated sooner or later. They widely vary in origin, size, shape, pigmentation, biological functions, etc. Microbial spores have a diameter ranging between 1-50 µm. However, the largest spores are the fungal ascospores, for eg. Varicellaria microsticta, measuring 340 x 115 µm; the smallest spores are bacterial endospores measuring about 0.25 µm in diameter. Bacterial endospores remain dormant in the same place of origin and help the species to pass through an unfavourable period; such spores are termed as resting spores. Also, there are dispersal spores, those of Ascomycetes, whose explosive asci bursts with a hydrostatic pressure getting scattered even upto distance of 40 cm (Wolken et al., 2003).


Wheat Bran Initial Moisture Content Spore Production Trichoderma Harzianum Beauveria Bassiana 


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  1. Adams TT, Eiteman MA & Hansel BM, 2002, Solid-state fermentation of broiler litter for production of biocontrol agents, Bioresource Technology, 82, 33–41.Google Scholar
  2. Alves SB & Pereira RM, 1989, Production of Metarhizium anisopliae (Metsch.) Sorok and Beauveria bassiana (Bals) Vuill in plastic trays, Ecossistema, 14, 188–192Google Scholar
  3. Areas JA, Diaz BM, Lecuona RE, 1999, Bioinsecticidal activity of conidia and dry mycelium preparations of two isolates of Beauveria bassiana against the sugarcane borer Diatraea saccharalis. Journal of Biotechnology, 67, 151–8Google Scholar
  4. Barranco-Florida JE, Alatorre-Rosas R, Gutierrez-Rojas, Viniegra-Gonzalez & Saucedo-Casteneda, 2002, Criteria for the selection of strains of entomopathogenic fungi Verticillium lecanii for solidstate cultivation, Enzyme and Microbial Tehnology, 30, 910–915.Google Scholar
  5. Burtet MJG, Silva ME & Diehl Fleig E, 1997, Prducao de conidios e micelio seco de Beauveria bassiana (Bals) Viull. para contrôle de formigas cortreiras, Congresso Bras Entomol, 16, 101.Google Scholar
  6. Calderon A, Fraga M & Carreras B, 1995, Production of Beauveria baasiana by solid-state fermentation, Reviews on Protection of Vegetables, 10, 269–273.Google Scholar
  7. Capalbo DMF, Moraes IO & Moraes RO, 1994, Development of a bioreactor for semi-solid fermentation purposes: Bacterial insecticide fermentation. In: Development in Food engineering. Proc. Int. Congress Eng. Food 6th 1993 (pub 1994), Yano T, Natanno R, Nakamura K (eds), Blackie, Glasgow, UK.Google Scholar
  8. Capalbo DMF, Moraes IO, 1988, Production of protein protoxin by Bacillus thuringiensis by semi-solid fermentation, In: 12th Simposio Annual da Academia de Ciencias do Fstado de Sao Paulo, Campinas 1988, Vol. 11, pp 46–55.Google Scholar
  9. Capalbo DMF, Valicente FH, Moraes IO & Peliser LH, 2001, Solid-state fermentation of Bacillus thuringiensis tolworthi to control fall armyworm in maize, Electronic journal of Biotechnology, 4, 2, 112–115Google Scholar
  10. Casula G, Cutting SM, 2002, Bacillus Probiotics: Spore germination in the gastrointestinal tract, Applied and Environmental Microbiology, 68, 5, 2344–2352.Google Scholar
  11. Chen W, Li Y, Du G & Chen J, 2005, Application of resposnse surface methodology in medium optimization for spore production of Coniothyrium minitans in solid-state fermentation, World Journal of Microbiology & Biotechnology, 21, 593–599Google Scholar
  12. Creuly C, Larroche C & Gros JB, 1990, A fed-batch technique for 2-heptanone production by spores of Pénicillium roquefortii, Applied Microbiology and Biotechnology, 34, 20–25.Google Scholar
  13. Dahlberg KR & Van etten JL, 1982, Physiology and biochemistry of fungal sporulation, Annual Review of Phytopathology, 20, 281–301.CrossRefGoogle Scholar
  14. De Reu JC, Solid-substrate fermentation of soya beans to Tempeh process innovations and product characteristics, 1995, Ph.D. Thesis, Wageningen Agricultural Universtiy.Google Scholar
  15. Deshchamps F & Huet FC, 1984, ?-glucosidase production in agitated solid fermentation, study of its properties, Biotechnology Letters, 6, 451–456.Google Scholar
  16. Desfarges C, Larroche C & Gros JB, 1987, Spore production of Penicillium roquefortii by solid state fermentation: Stoichiometry, growth and sporulation behaviour, Biotechnology and Bioengineering, 29, 1050–1058.Google Scholar
  17. Desgranges C, Vergoignan C, Lereec A, Riba G & Durrand A, 1993, Use of solid state fermentatation to produce Beauveria bassiana for the biological control of European corn borer, Biotechnology Advances, 11(3), 577–587.CrossRefGoogle Scholar
  18. Devi PSV, Ravinder T & Jaidev C, 2005, Cost-effective production of Bacillus thuringiensis by solid-state fermentation, Journal of Invertebrate Pathology, 88, 163–168.CrossRefGoogle Scholar
  19. Dijksterhuis J & Samson RA, 2002, Food and crop spoilage on storage. In Mycota XI Agricultural Applications (Kemken, F., ed), pp 39–52, Springer-Verlag.Google Scholar
  20. Dorta B & Arcas J, 1998, Sporulation of Metarhizium anisopliae in solid-state fermentation with forced aeration, Enzyme and Microbial Technology, 23, 501–505.CrossRefGoogle Scholar
  21. Durand A, Renaud R, Maratray J, Almanza S & Pelletier A, 1994, Reactor for sterile solid-state fermentation methods, Patent WO 94/18306.Google Scholar
  22. Feng KC, Liu BL & Tzeng YM, 2000, Verticillium lecanii spore production in solid-state and liquid-state fermentations, Bioprocess Engineering, 23, 25–29CrossRefGoogle Scholar
  23. Feng KC, Liu BL & Tzeng YM, 2002, Morphological characterization and germination of aerial and submerged spores of the entompathogenic fungus Verticillium lecanii, World Journal of Microbiology & Biotechnology, 18, 217–224.CrossRefGoogle Scholar
  24. Ferron P, 1978, Biological control of insect pests by entomogenous fungi, Annual Review of Entomology, 23, 409–442.CrossRefGoogle Scholar
  25. Foda MS, El-Bendary MA & Moharam ME, 2003, Salient parameters involved in mosquitocidal toxins production from Bacillus spharicus by semi-solid substrate fermentation, Egypt Microbiology, 38, 229–246.Google Scholar
  26. Foda MS, Ismail IMK, Moharam ME & Sadek Kh. HA, 2002, A novel approach for production of Bacillus thuringiensis by solid-state fermentation, Egypt Microbiology, 37, 135–156.Google Scholar
  27. Gooday GW, 1981, Biogenesis of sporopollenin in fungal spore walls. In the Fungal spores; Morphogenetic controls, ed, Turian G & Hohl, HK, New York: Academic Press, pp 487–505, ISBN 0-12-703680-0.Google Scholar
  28. Grajek W, 1994, Sporogenesis of the entomopathogenic fungus Verticillium lecanii in solid-state cultures, Folia Microbiologica, 39, 29–32.CrossRefGoogle Scholar
  29. Holker U, 2000, Bioreactor for fermenting solids, Patent PCT WO 01/19954.Google Scholar
  30. Hussley NW & Tinsley, 1981, Impression of insect pathology in the People’s Republic of China. In: Surges, HD (Ed.), Microbial control of Pests and Plant Diseases 1970-1980. Academic Press, New York, pp 785–796.Google Scholar
  31. Lareo C, Sposito AF & Bossio AL, 2006 Volpe DC, Characterization of growth and sporulation of Mucor bacilliformis in solid state fermentation on an inert support, Enzyme and Microbial Technology, 38, 391–399.Google Scholar
  32. Larroche C & Gros JB, 1989, Strategies for spore production by Penicillium roquefortii using solid state fermentation techniques, Process Biochemistry, 24(2), 97–103.Google Scholar
  33. Larroche C & Gros JB, 1992, Characterization of the growth and sporulation behaviour of Penicillium roquefortii in solid substrate fermentation by material and bioenergetic balances, Biotechnology and Bioengineering, 39, 815–827.CrossRefGoogle Scholar
  34. Larroche C & Gros JB, 1997, Special transformation processes using fungal spores and immobilized cells, Advances in Biochemical Engineering and Biotechnology, 55, 179–220.Google Scholar
  35. Larroche C, Desfarges C & Gros JB, 1986, Spore production of Penicillium roquefortii in simulated solid state fermentation, Biotechnology Letters, 8(6), 453–456CrossRefGoogle Scholar
  36. Larroche C, Desfarges C & Gros JB, 1988, Optimization of the spore production of Pénicillium roquefortii in solid substrate fermentation on buckwheat seeds, Applied Microbiology Biotechnology, 28, 85–92Google Scholar
  37. Leuenberger HGW, 1984, Methodology. In; Biotechnology, Vol. 6a, Kieslick (eds), VErlag Chemie, Weinheim, Deerfield Beach, Basel, pp 5–9.Google Scholar
  38. Lonsane BK, Ghildayal NP, Budiatman S & Ramakrishna SV, 1985, Engineering aspects of solid state fermentation, Enzyme and Microbial Technology, 7, 258–265CrossRefGoogle Scholar
  39. McCoy CW, Stamper DH & Tuveson RW, 1984, Conidiogenous cell difference among mutant and wild type pathotypes of Hirsutella thompsonii var. thompsonii, Journal of Invertebrate Pathology, 43, 414–421.CrossRefGoogle Scholar
  40. McQuiken MP & Whipps JM, 1995, Production, survival and evaluation of solid-substrate inocula of Coniothyrium minitans against Sclerotinia sclerotiorum, European Journal of Plant Pathology, 101, 101–110CrossRefGoogle Scholar
  41. McQuilken MP, Budge SP & Whipps JM, 1997, Production, survival and evaluation of liquid culture produced inocula of Coniothyrium minitans against Sclerotinina sclerotiorum, Biocontrol Science and Technology, 7, 23–26.CrossRefGoogle Scholar
  42. Minton NP, Mauchline ML, Lemmon MJ, Brehm JK, Fox M, Michael NP, Giaccia A & Brown JM, 1995, Chemotherapeutic tumor targeting using clostridial spores, FEMS Microbial Reviews, 17, 357–364.CrossRefGoogle Scholar
  43. Mitchell DA, von Meien OF, Luz Jr LFL & Krieger N, 2002, Evaluation of productivity of zymotis solid-state fermentation bioreactor based on total reactor volume, Food Technology & Biotechnology, 40(2), 135–144.Google Scholar
  44. Moksia, J, Larroche, C & Gros JB, 1996, Gluconate production by spores of Aspergillus niger. Biotechnology Letters 18, 1025–1030.CrossRefGoogle Scholar
  45. Moo-Young M, Moreira AR & Tengerdy RP, 1983, Principles of solid-substrate fermentation, In; JE Smith, DR Berry, B Kristiansen (ed), The filamentous fungi, vol 4 (Fungal Technology), Edward Arnold, London, pp 117–144.Google Scholar
  46. Mose JR, 1970, The use of spores of bacteria for a serological tumor diagnosis (I), Z Krebsforsch, 72, 329–341.Google Scholar
  47. Moskowitz GJ, 1979, Inocula for blue-veined chesses and blue cheese flavour, pp 201–210, In: Peppier AJ, Perlman D (eds), Microbial Technology, Vol. 2, 2nd edition, Academic, New York & London.Google Scholar
  48. Munoz GA, Agosin E, Cotoras M, San Martin R & Volpe D, 1995, Comparison of aerial and submerged spore properties for Trichoderma harzianum, FEMS Microbiology Letters, 125, 63–70.CrossRefGoogle Scholar
  49. Oostra J, Tramper J & Rinzema A, 2000, Model-based bioreactor selection for large-scale solidstate cultivation of Coniothyrium minitans spores on oats, Enzyme and Microbial Technology, 27, 652–663.CrossRefGoogle Scholar
  50. Pandey A, 1992. Recent developments in solid-state fermentation, Process Biochemistry, 27, 109–117.Google Scholar
  51. Pandey A, 1994, Solid-state fermentation: An overview. In: Pandey, A. (Ed.), Solid-State Fermentation, Wiley Eastern, New Delhi, pp. 3–10.Google Scholar
  52. Pandey A, 2003, Solid-state fermentation, Biochemical Engineering Journal, 13(2-3), 81–84.CrossRefGoogle Scholar
  53. Pandey A, Selvakumar P, Ashakumary L & Damodaran AD, 1996, A novel process for the production of fungal spores in solid-state fermentation.. Indian patent IPA no 379/ DEL/96, Dated 23.2.96.Google Scholar
  54. Pandey A, Soccol CR & Mitchell D, 2000, New developments in solid-state fermentation: I-bioprocesses and products, Process Biochemistry, 35(10), 1153–1169.CrossRefGoogle Scholar
  55. Pascual S, De Cal A, Magan N & Melgarejo P, 2000, Surface hydrophobicity, viability and efficacy in biological control of Penicillium oxalicum spores produced in aerial and submerged culture, Journal of Applied Microbiology, 89, 847–853.CrossRefGoogle Scholar
  56. Raimbault M, 1981, Solid-state fermentation: growth of filamentous fungi in starchy substrate. ORSTOM 127.Google Scholar
  57. Ramachandran S, Fontanille P, Pandey A & Larroche C, 2007a, Spores of Aspergillus niger as reservoir of glucose oxidase synthesized during solid-state fermentation and their use as catalyst in gluconic acid production, Letters in Applied Microbiology, 44, 155–160.CrossRefGoogle Scholar
  58. Ramachandran S, Fontanille P, Pandey A & Larroche C, 2007b, Stability of glucose oxidase activity of A. niger spores produced by solid-state fermentation and its role as biocatalyst in bioconversion reaction, Food Technology and Biotechnology.Google Scholar
  59. Rotamn B, 2001, Using living spores for real-time biosensing, Genetic Engineering News, 21, 21.Google Scholar
  60. Roussos S, Olmas A, Raimbault M, Saucedo-Castaneda G & Lonsane BK, 1991, Startegies for large scale inoculum development for solid state fermentation system: Conidiospores of Trichoderma harzianum, Biotechnology Techniques, 5(6), 415–420.CrossRefGoogle Scholar
  61. Roussos S, Raimbault M, Prebois JP & Lonsane BK, 1993, Zymotis, a large scale solid state fermenter: design and evaluation, Applied Biochemistry and Biotechnology, 42, 37–52.CrossRefGoogle Scholar
  62. Santa HSD, Santa ORD, Brand D, Vandenberghe LPS & Soccol CR, 2005, Spore production of Beauveria bassiana from agro-industrial residues, Brazilian Archives of Biology and Technology, 48, 51–60.CrossRefGoogle Scholar
  63. Setlow P, 2001, Resistance of bacterial spores to ultraviolet light, Environmental Molecular Mutagenesis, 38, 97–104.CrossRefGoogle Scholar
  64. Silman RW, 1980, Enzyme formation during solid-substrate fermentation in rotating vessels, Biotechnology and Bioengineering, 22, 411–420.CrossRefGoogle Scholar
  65. Silman RW, Bothast RJ & Schisler DA, 1993, Production of Colletotrichumtruncatum for use as a mycoherbicide: Effects of culture, drying and storage on recovery and efficacy, 11, 561–575.Google Scholar
  66. Soccol CR, Ayala L, Soccol VT, Krueger N & Santos HR, 1997, Spore production by entomopathogenic fungus Beauveria bassiana from declassified potato by solid-state fermentation, Reviews in Microbiology, 28, 34–42.Google Scholar
  67. Sphicer G, Peters J, Nürnberg M & Schwebke I, 2002, Microbicidal efficacy of superheated steam II, Studies involving E. faecium and spores of B. xerothermodurans and B. coagulans, International Journal of Hygiene and Environmental Health, 204, 309–316.CrossRefGoogle Scholar
  68. Tarocco F, Lecuona RE, Couto AS & Areas JA, 2005, Optimization of erythritol and glycerol accumulation in conidia of Beauveria bassiana by solid-state fermentation using response surface methodology, Applied Microbiology and Biotechnology, 68, 481–488.CrossRefGoogle Scholar
  69. Viccim G, Mannich M, Capalbo DFM, Valdebenito-Sanhueza RA & Mitchell DA, 2006 Spore production in solid-state fermentation of rice by Clonostachys rosea, a biopesticide for gray mold of strawberries, Process Biochemistry.Google Scholar
  70. Vilas Boas AM, Andrade RM & Oliveira JV, 1996, Diversificacao de meios de cultura para producao de fungos entomotogenicos, Archives of Biology and Technology, 39, 123–128.Google Scholar
  71. Wang T, 1988, A simple and economic semi-solid fermentation process for Bacillus thuringiensis, International symposium on Insecticide of Bacillus thuringiensis. Hubei Academy of Agricultural Science, Wuhan, October, 1988.Google Scholar
  72. Weber FJ, Tramper J & Rinzema A, 1999, A simplified material and energy balance approach for process development and scale-up of Coniothyrium minitans conidia production by solid-state fermentation cultivation in a packed bed reactor, Biotechnology and Bioengineering, 65(4), 447–458.CrossRefGoogle Scholar
  73. Wolken WAM, Tramper J & van der Derf MJ, 2003, What spores can do for us, Trends in Biotechnology, 21(8), 338–345.CrossRefGoogle Scholar
  74. Wolken WAM, Tramper J & van de Werf MJ, 2002, Toxicity of terpenes to spores and mycelium of Penicillium digitatum, Biotechnology and Bioengineering, 80, 685–690.CrossRefGoogle Scholar
  75. Wolken WAM & van der Werf, 2001, Geraniol biotransformation-pathway in spores of Penicillium digitatum, Applied Microbiology and Biotechnology, 57, 731–737.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Sumitra Ramachandran
    • 1
  • Christian Larroche
    • 1
  • Ashok Pandey
    • 2
  1. 1.Laboratoire de Génie Chimique et Biochimique (LGCB)CUST-Université Biaise PascalAubière CedexFrance
  2. 2.Bioprocess Engineering and Biotechnology DivisionFederal University of ParanáCuritiba-PRBrazil

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