Fiber Optic Biosensors for Bacterial Detection
Rapid and specific identification of bacteria is critical for clinical and biosafety applications. Fiber optic biosensors (FOBs) are increasingly being applied to the detection of bacteria in food and water supplies, food processing facilities, and homeland security operations. These biosensors can be used for multiplexed pathogen detection or to confirm the results of other techniques, often in less than one hour. FOBs offer several advantages over conventional culture-based techniques, or polymerase chain reaction (PCR)-based assays, in terms of speed, specificity, and depth of information content. In addition, some sensor platforms have been developed into portable systems capable of emergency field deployment. In this chapter, we will discuss the detection of bacteria using fiber optic immunosensors, nucleic acid-based FOBs in various assay formats, and several applications of these technologies.
KeywordsEvanescent Wave Francisella Tularensis Sandwich Assay Bacterial Detection Bead Type
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- Anderson GP, Rowe-Taitt CA and Ligler FS (2000) Raptor: A portable, automated biosensor. Proceedings of the First Conference on Point Detection for Chemical and Biological DefenseGoogle Scholar
- DeMarco DR and Lim DV (2002a) Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides. J. Food Prot. 65:596–602Google Scholar
- DeMarco DR and Lim DV (2002b) Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with slica and polystyrene waveguides. J. Food Prot. 65:596–602Google Scholar
- DeMarco DR, Saaski EW, McCrae DA and Lim DV (1999) Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor. J. Food Prot. 62:711–716Google Scholar
- Fan J-B, Oliphant A, Shen R, Kermani BG, Garcia F, Gunderson KL, Hansen M, Steemers F, Butler SL, Deloukas P, Galver L, Hunt S, McBride C, Bibikova M, Rubano T, Chen J, Wickham E, Doucet D, Chang W, Campbell D, Zhang B, Kruglyak S, Bentley D, Haas J, Rigault P, Zhou L, Stuelpnagel J and Chee MS (2003) Highly parallel SNP genotyping. Cold Spring Harbor Symp. Quant. Biol. LXVIII:69–78Google Scholar
- Garaizar J, Rementeria A and Porwollik S (2006) DNA microarray technology: A new tool for the epidemiological typing of bacterial pathogens? FEMS Immunol. Med. Microbiol. 47:178–189Google Scholar
- Kramer MF and Lim DV (2004) A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seeds. J. Food Prot. 67:46–52Google Scholar
- Peiffer DA, Le JM, Steemers FJ, Chang W, Jenniges T, Garcia F, Haden K, Li J, Shaw CA, Belmont J, Cheung SW, Shen RM, Barker DL and Gunderson KL (2006) High-resolution genomic profiling of chromosomal aberrations using infinium whole-genome genotyping. Genome Research. 16:1136–1148CrossRefGoogle Scholar
- Tims TB, Dickey SS, DeMarco DR and Lim DV (2001) Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. American Clinical Laboratory. 28–29Google Scholar