Fiber Optic Biosensors for Bacterial Detection

  • Ryan B. Hayman


Rapid and specific identification of bacteria is critical for clinical and biosafety applications. Fiber optic biosensors (FOBs) are increasingly being applied to the detection of bacteria in food and water supplies, food processing facilities, and homeland security operations. These biosensors can be used for multiplexed pathogen detection or to confirm the results of other techniques, often in less than one hour. FOBs offer several advantages over conventional culture-based techniques, or polymerase chain reaction (PCR)-based assays, in terms of speed, specificity, and depth of information content. In addition, some sensor platforms have been developed into portable systems capable of emergency field deployment. In this chapter, we will discuss the detection of bacteria using fiber optic immunosensors, nucleic acid-based FOBs in various assay formats, and several applications of these technologies.


Evanescent Wave Francisella Tularensis Sandwich Assay Bacterial Detection Bead Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ahn S, Kulis DM, Erdner DL, Anderson DM and Walt DR (2006) Fiber-optic microarray for simultaneous detection of multiple harmful algal bloom species. Appl. Environ. Microbiol. 72:5742–5749CrossRefGoogle Scholar
  2. Ahn S and Walt DR (2005) Detection of Salmonella spp. using microsphere-based, fiber-optic DNA microarrays. Anal. Chem. 77:5041–5047CrossRefGoogle Scholar
  3. Anderson GP, Rowe-Taitt CA and Ligler FS (2000) Raptor: A portable, automated biosensor. Proceedings of the First Conference on Point Detection for Chemical and Biological DefenseGoogle Scholar
  4. Bhunia AK, Banada P, Banerjee P, Valadez A and Hirleman ED (2007) Light scattering, fiber optic- and cell-based sensors for sensitive detection of foodborne pathogens. Journal of Rapid Methods and Automation in Microbiology. 15:121–145CrossRefGoogle Scholar
  5. Brogan KL and Walt DR (2005) Optical fiber-based sensors: Application to chemical biology. Current Opinion in Chemical Biology. 9:494–500CrossRefGoogle Scholar
  6. DeMarco DR and Lim DV (2001) Direct detection of Escherichia coli O157:H7 in unpasteurized apple juice with an evanescent wave biosensor. Journal of Rapid Methods and Automation in Microbiology. 9:241–257CrossRefGoogle Scholar
  7. DeMarco DR and Lim DV (2002a) Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides. J. Food Prot. 65:596–602Google Scholar
  8. DeMarco DR and Lim DV (2002b) Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with slica and polystyrene waveguides. J. Food Prot. 65:596–602Google Scholar
  9. DeMarco DR, Saaski EW, McCrae DA and Lim DV (1999) Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor. J. Food Prot. 62:711–716Google Scholar
  10. Epstein JR, Lee M and Walt DR (2002) High-density fiber-optic genosensor microsphere array capable of zeptomole detection limits. Anal. Chem. 74:1836–1840CrossRefGoogle Scholar
  11. Epstein JR, Leung APK, Lee K-H and Walt DR (2003) High-density, microsphere-based fiber optic DNA microarrays. Biosensors and Bioelectronics. 18:541–546CrossRefGoogle Scholar
  12. Fan J-B, Oliphant A, Shen R, Kermani BG, Garcia F, Gunderson KL, Hansen M, Steemers F, Butler SL, Deloukas P, Galver L, Hunt S, McBride C, Bibikova M, Rubano T, Chen J, Wickham E, Doucet D, Chang W, Campbell D, Zhang B, Kruglyak S, Bentley D, Haas J, Rigault P, Zhou L, Stuelpnagel J and Chee MS (2003) Highly parallel SNP genotyping. Cold Spring Harbor Symp. Quant. Biol. LXVIII:69–78Google Scholar
  13. Ferguson JA, Steemers FJ and Walt DR (2000) High-density fibre-optic DNA random microsphere array. Anal. Chem. 72:5618–5624CrossRefGoogle Scholar
  14. Ferreira AP, Werneck MM and Ribeiro RM (1999) Aerobiological pathogen detection by evanescent wave fiber optic sensor. Biotechnol. Tech. 13:447–452CrossRefGoogle Scholar
  15. Ferreira AP, Werneck MM and Ribeiro RM (2001) Development of an evanescent-field fiber optic sensor for Escherichia coli O157:H7. Biosensors and Bioelectronics. 16:399–408CrossRefGoogle Scholar
  16. Garaizar J, Rementeria A and Porwollik S (2006) DNA microarray technology: A new tool for the epidemiological typing of bacterial pathogens? FEMS Immunol. Med. Microbiol. 47:178–189Google Scholar
  17. Geng T, Morgan MT and Bhunia AK (2004) Detection of low levels of Listeria monocytogenes cells by using a fiber-optic immunosensor. Appl. Environ. Microbiol. 70:6138–6146CrossRefGoogle Scholar
  18. Geng T, Uknalis J, Tu S-I and Bhunia AK (2006) Fibre-optic biosensor employing alexa-fluor conjugated antibody for detection of Escherichia coli O157:H7 from ground beef in four hours. Sensors. 6:796–807CrossRefGoogle Scholar
  19. Gunderson KL, Steemers FJ, Lee G, Mendoza LG and Chee MS (2005) A genome-wide scalable SNP genotyping assay using microarray technology. Nature Genetics. 37:549–554CrossRefGoogle Scholar
  20. Gunderson KL, Steemers FJ, Ren H, Ng P, Zhou L, Tsan C, Chang W, Bullis D, Musmacker J, King C, Lebruska LL, Barker D, Oliphant A, Kuhn KM and Shen R (2006) Whole-genome genotyping. Methods Enzymol. 410:359–376CrossRefGoogle Scholar
  21. Iqbal SS, Mayo MW, Bruno JG, Bronk BV, Batt CA and Chambers JP (2000) A review of molecular recognition technologies for detection of biological threat agents. Biosensors and Bioelectronics. 15:549–578CrossRefGoogle Scholar
  22. King KD, Vanniere JM, Leblanc JL, Bullock KE and Anderson GP (2000) Automated fiber optic biosensor for multiplexed immunoassays. Environ. Sci. Technol. 34:2845–2850CrossRefGoogle Scholar
  23. Kramer MF and Lim DV (2004) A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seeds. J. Food Prot. 67:46–52Google Scholar
  24. Lammerding AM and Fazil A (2000) Hazard identification and exposure assessment for microbial food safety risk assessment. International Journal of Food Micriobiology. 58:147–157CrossRefGoogle Scholar
  25. Ligler FS, Sapsford KE, Golden JP, Shriver-Lake LC, Taitt CR, Dyer MA, Barone S and Myatt CJ (2007) The array biosensor: Portable, automated systems. Anal. Sci. 23:5–10CrossRefGoogle Scholar
  26. Mothershed EA and Whitney AM (2006) Nucleic acid-based methods for the detection of bacterial pathogens: Present and future considerations for the clinical laboratory. Clin. Chim. Acta. 63:206–220CrossRefGoogle Scholar
  27. Peiffer DA, Le JM, Steemers FJ, Chang W, Jenniges T, Garcia F, Haden K, Li J, Shaw CA, Belmont J, Cheung SW, Shen RM, Barker DL and Gunderson KL (2006) High-resolution genomic profiling of chromosomal aberrations using infinium whole-genome genotyping. Genome Research. 16:1136–1148CrossRefGoogle Scholar
  28. Rowe-Taitt CA, Hazzard JW, Hoffman KE, Cras JJ, Golden JP and Ligler FS (2000) Simultaneous detection of six biohazardous agents using a planar waveguide array biosensor. Biosensors and Bioelectronics. 15:579–589CrossRefGoogle Scholar
  29. Shepard JRE, Danin-Poleg Y, Kashi Y and Walt DR (2005) Array-based binary analysis for bacterial typing. Anal. Chem. 77:319–326CrossRefGoogle Scholar
  30. Simpson JM and Lim DV (2005) Rapid pcr confirmation of E. coli O157:H7 after evanescent wave fibre optic biosensor detection. Biosensors and Bioelectronics. 21:881–887CrossRefGoogle Scholar
  31. Song L, Ahn S and Walt DR (2006) Fibre-optic microsphere-based arrays for multiplexed biological warfare agent detection. Anal. Chem. 78:1023–1033CrossRefGoogle Scholar
  32. Steemers FJ, Chang W, Lee G, Barker DL, Shen R and Gunderson KL (2006) Whole-genome genotyping with the single-base extension assay. Nature Methods. 3:31–33CrossRefGoogle Scholar
  33. Taitt CR, Anderson GP and Ligler FS (2005) Evanescent wave fluorescence biosensors. Biosensors and Bioelectronics. 20:2470–2487CrossRefGoogle Scholar
  34. Taitt CR, Shubin YS, Angel R and Ligler FS (2004) Detection of Salmonella enterica serovar Typhimurium by using a rapid, array-based immunosensor. Appl. Environ. Microbiol. 70:152–158CrossRefGoogle Scholar
  35. Tims TB, Dickey SS, DeMarco DR and Lim DV (2001) Detection of low levels of Listeria monocytogenes within 20 hours using an evanescent wave biosensor. American Clinical Laboratory. 28–29Google Scholar
  36. Tims TB and Lim DV (2003) Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection. Journal of Microbiological Methods. 55:141–147CrossRefGoogle Scholar
  37. Tims TB and Lim DV (2004) Rapid detection of Bacillus anthracis spores direcly from powders with an evanescent wave fibre-optic biosensor. Journal of Microbiological Methods. 59:127–130CrossRefGoogle Scholar
  38. Zourob M, Mohr S, Brown BJT, Fielden PR, McDonnell MB and Goddard NJ (2005a) Bacteria detection using disposable optical leaky waveguide sensors. Biosensors and Bioelectronics. 21:293–302CrossRefGoogle Scholar
  39. Zourob M, Mohr S, Brown BJT, Fielden PR, McDonnell MB and Goddard NJ (2005b) An integrated metal clad leaky waveguide sensor for detection of bacteria. Anal. Chem. 77:232–242CrossRefGoogle Scholar
  40. Zourob M, Mohr S, Brown BJT, Fielden PR, McDonnell MB and Goddard NJ (2005c) An integrated optical leaky waveguide sensor with electrically induced concentration system for the detection of bacteria. Lab on a Chip. 5:1360–1365CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Ryan B. Hayman
    • 1
  1. 1.Walt Lab Department of ChemistryTufts UniversityUSA

Personalised recommendations