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Detection and Viability Assessment of Endospore-Forming Pathogens

  • Adrian Ponce
  • Stephanie A. Connon
  • Pun To Yung

Abstract

In this chapter, we explore technology developments for the rapid detection, identification, and viability assessment of endospore-forming pathogens with a focus on Bacillus anthracis. First, we introduce various toxin-producing species and their role as bioinsecticides, probiotics, and bioweapons. We also review the role of endospores as biological indicators (i.e., dosimeters) for evaluating sterilization regimens, such as autoclaving and wastewater remediation. Monitoring the effectiveness of cleaning and sterilization regimens to maintain good hygiene is required in several major industries, including health care, food, and pharmaceutical industries. In the next section, we review recent developments in DNA-, immuno-, and dipicolinic acid assays, and their applications for detection and monitoring of Bacillus anthracis and other endospore-forming pathogens. Finally, we review viability assays capable of rapid validation of endospore inactivation after sterilization, including assays based on ATP synthesis during stage II germination, and DPA release during stage I germination.

Keywords

Bacillus Cereus Multiplex Polymerase Chain Reaction Clinical Microbiology Environmental Microbiology Bacillus Anthracis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Adrian Ponce
    • 1
  • Stephanie A. Connon
    • 1
  • Pun To Yung
    • 1
  1. 1.California Institute of Technology, 91125/Jet Propulsion LaboratoryPasadena

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