Microbial Genetic Analysis Based on Field Effect Transistors

  • Yuji Miyahara
  • Toshiya Sakata
  • Akira Matsumoto


In this chapter, potentiometric detection methods for microbial DNA involved recognition events by use of genetic field effect devices will be described. Fundamental principles of field effect devices and the technical background with their ongoing applications in the field of bio-sensor technologies, termed bio-FET, will be first introduced. Then concept of genetic field effect transistor will be described with emphasis on their fabrication, characteristics, and recent applications to microbial Single Nucleotide Polymorphysms (SNPs) Analysis as well as DNA sequencing. By comparing to other conventional methods, technical significance and future perspective of the genetic field effect transistor will also be discussed in detail.


Oligonucleotide Probe Field Effect Transistor Gate Insulator Single Nucleotide Polymorphism Genotyping Extension Reaction 


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yuji Miyahara
    • 1
    • 2
  • Toshiya Sakata
    • 2
  • Akira Matsumoto
    • 2
  1. 1.Biomaterials Center National Institute for Materials Science 1-1 NamikiJapan
  2. 2.Department of Materials EngineeringGraduate School of Engineering The University of TokyoTokyoJapan

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