Advertisement

Highly Sensitive Rapid, Reliable, and Automatic Cardiovascular Disease Diagnosis with Nanoparticle Fluorescence Enhancer and Mems

  • Bin Hong
  • Junhai Kai
  • Yongjie Ren
  • Jungyoup Han
  • Zhiwei Zou
  • Chong H. Ahn
  • Kyung A. Kang
Part of the Advances In Experimental Medicine And Biology book series (AEMB, volume 614)

Abstract

Cardiovascular diseases (CVDs) have been the leading threat to human life. An effective way for sensitive and accurate CVD diagnosis is to measure the biochemical markers released from the damaged myocardial cells in the bloodstream. Here, a multi-analyte, fluorophore mediated, fiber-optic immuno-biosensing system is being developed to simultaneously and rapidly quantify four clinically important cardiac markers, myoglobin, C-reactive protein, cardiac troponin I, and B-type natriuretic peptide. To quantify these markers at a pico-molar level, novel nanoparticle reagents enhancing fluorescence were used and signal enhancement was obtained as high as ~230%. Micro-electro-mechanical system (MEMS) was integrated to this system to ensure a reliable and fully-automated sensing performance. A point-of-care, automatic microfluidic sensing system for four cardiac marker quantification was developed with the properties of 3 cm sensor size, 300 μL sample volume, 9-minute assay time, and an average signal-to-noise ratio of 35.

Keywords

Cardiac Troponin Cardiac Marker Cyclic Olefin Copolymer Sample Incubation Sensor Size 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    American Heart Association. Heart Disease and Stroke Statistics, Update, 10–12 (2005).Google Scholar
  2. 2.
    F. S. Apple, R. H. Christenson, R. Valdes, A. J. Andriak, K. Mascotti, and A. H.B. Wu, Simultaneous rapid measurement of whole blood myoglobin, creatine kinase MB, and cardiac troponin I by the triage cardiac panel for detection of myocardial infarction, Clin. Chem. 45(2), 199–205 (1999).PubMedGoogle Scholar
  3. 3.
    A. S. Maisel, P. Krishnaswamy, H. C. Herrmann, and P. A. McCullough, Rapid measurement of B-type natriuretic peptide in the emergency diagnosis of heart failure, New Engl. J. Med. 347, 161–167 (2002).PubMedCrossRefGoogle Scholar
  4. 4.
    M. S. Sabatine , D. A. Morrow, C. P. Cannon, and E. Braunwald, Multimarker approach to risk stratification in non-ST elevation acute coronary syndromes: Simultaneous assessment of troponin I, c-reactive protein, and b-type natriuretic peptide, Circ. 105, 1760–1763 (2002).CrossRefGoogle Scholar
  5. 5.
    L. Tang, Y. J. Ren, B. Hong, and K. A. Kang, A fluorophore-mediated, fiber-optic, multi-analyte, immuno-sensing system for rapid diagnosis and prognosis of cardiovascular diseases, J. Biomed. Optics 11, 021011 (2006).CrossRefGoogle Scholar
  6. 6.
    B. Hong and K. A. Kang, Biocompatible, nanogold-particle fluorescence enhancer for fluorophore mediated, optical immunosensor, Biosens. Bioelectron. 21(7), 1333–1338 (2006).PubMedCrossRefGoogle Scholar
  7. 7.
    K. A. Kang and B. Hong, Biocompatible nano-metal particle fluorescence enhancers, Crit. Rev. Eukar. Gene Expres. 16(1), 45–60 (2006).Google Scholar
  8. 8.
    L. Tang, 2005. Multi-analyte, fiber-optic immuno-biosensing system for rapid disease diagnosis: model systems for anticoagulants and cardiac markers. Dissertation. Chemical Engineering, University of Louisville, Louisville, KY.Google Scholar
  9. 9.
    Y. Sohn, J. H. Kai, C. H. Ahn, Protein array patterning on Cyclic Olefin Copolymer (COC) for disposable protein chip, Sensor Lett. 2, 171–174 (2005).CrossRefGoogle Scholar
  10. 10.
    L. Tang, H. J. Kwon, and K. A. Kang, Theoretical and experimental analysis of analyte transport in a fiber optic, protein C immuno-biosensor,Biotech. Bioeng. 88, 869–879 (2004).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Bin Hong
    • 1
  • Junhai Kai
    • 2
  • Yongjie Ren
    • 1
  • Jungyoup Han
    • 2
  • Zhiwei Zou
    • 2
  • Chong H. Ahn
    • 2
  • Kyung A. Kang
    • 1
  1. 1.Department of Chemical EngineeringUniversity of LouisvilleLouisville
  2. 2.Department of Electrical and Computer Engineering and Computer ScienceUniversity of CincinnatiCincinnati

Personalised recommendations