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Lentiviral Gene Transfer-Mediated Cone Vision Restoration in RPE65 Knockout Mice

  • Alexis-Pierre Bemelmans
  • Corinne Kostic
  • Maité Cachafeiro
  • Sylvain V. Crippa
  • Dana Wanner
  • Meriem Tekaya
  • Andreas Wenzel
  • Yvan Arsenijevic
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 613)

In photoreceptors, the photo-isomerization of 11-cis-retinal chromophore into alltrans-retinal is the first step of the photo-transduction cascade. To regain light sensitivity after light exposure, photoreceptors need to recycle their chromophore. This is done through the retinoid visual cycle, which involves a series of enzymatic reactions taking place in photoreceptors as well as in the cells of the retinal-pigmented epithelium (RPE) (reviewed by JC Saari (Saari, 2000)). Rpe65, a gene specifically expressed in RPE, has recently been identified as the isomerohydrolase (Jin et al., 2005; Moiseyev et al., 2005), one of the key enzymes of the retinoid visual cycle. As a consequence, when Rpe65 is mutated, the retinoid visual cycle is disrupted, and the sensitivity to light of the photoreceptors is drastically reduced.

Keywords

Retinal Pigment Epithelium Cone Photoreceptor Intraocular Injection Optomotor Response Photopic Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Alexis-Pierre Bemelmans
    • 1
  • Corinne Kostic
    • 2
  • Maité Cachafeiro
    • 3
  • Sylvain V. Crippa
    • 4
  • Dana Wanner
    • 5
  • Meriem Tekaya
    • 6
  • Andreas Wenzel
    • 7
  • Yvan Arsenijevic
    • 8
  1. 1.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  2. 2.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  3. 3.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  4. 4.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  5. 5.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  6. 6.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland
  7. 7.Laboratory for Retinal Cell BiologyDept. of Ophthalmology, University of ZürichZürichSwitzerland
  8. 8.Unit of Gene Therapy and Stem Cells BiologyHôpital Ophtalmique Jules-GoninLausanneSwitzerland

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