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An in-vivo Assay to Identify Compounds Protective Against Light Induced Apoptosis

  • Yogita Kanan
  • Anne Kasus Jacobi
  • Kjell Sawyer
  • David S. Mannel
  • Joyce Tombran Tink
  • Muayyad R. Al-Ubaidi
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 613)

Age-related macular degeneration (AMD) is the major cause of blindness in the elderly. Vision loss is caused by the death of cone photoreceptor cells in the macula. Approximately 1.65 million Americans over the age of 50 have the disease (Prevent Blindness America). Very little is known about the causes and mechanisms of photoreceptor death in this condition.

Keywords

Photoreceptor Cell Light Stress Pigment Epithelium Derive Factor Light Damage Bonferroni Multiple Comparison 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Al-Ubaidi, M.R., Font, R.L., Quiambao, A.B., Keener, M.J., Liou, G.I., Overbeek, P.A., and Baehr,W., 1992, Bilateral retinal and brain tumors in transgenic mice expressing simian virus 40 large T antigen under control of the human interphotoreceptor retinoid-binding protein promoter. J. Cell Biol. 119:1681.PubMedCrossRefGoogle Scholar
  2. Cao, W., Tombran-Tink, J., Elias, R; Sezate, S., Mrazek, D., and McGinnis, J.F., 2001, In vivo protection of photoreceptors from light damage by pigment epithelium-derived factor. Invest Ophthalmol. Vis. Sci. 42:1646.PubMedGoogle Scholar
  3. Imai, D., Yoneya, S., Gehlbach, P.L., Wei, L.L., and Mori, K., 2005, Intraoculargene transfer of pigment epithelium-derived factor rescues photoreceptors from light-induced cell death. J. Cell Physiol. 202:570.PubMedCrossRefGoogle Scholar
  4. Janecke, A.R., Thompson, D.A., Utermann, G., Becker, C., Hubner, C.A., Schmid, E., McHenry, C.L., Nair, A.R., Ruschendorf, F., Heckenlively, J., Wissinger, B., Nurnberg, P., and Gal, A., 2004, Mutations in RDH12 encoding a photoreceptor cell retinol dehydrogenase cause childhood-onset severe retinal dystrophy. Nat. Genet. 36:850.PubMedCrossRefGoogle Scholar
  5. Kanan, K., Moiseyev, G., Agarwal, N., Ma., J.X., and Al-Ubaidi, M.R., 2007, Light induces programmed cell death by activating multiple independent proteases in a cone photoreceptor cell line. Invest. Ophthalmol. Vis. Sci 48:40.PubMedCrossRefGoogle Scholar
  6. Maeda, A., Maeda, T., Imanishi, Y., Sun, W., Jastrzebska, B., Hatala, D.A., Winkens, H.J., Hofmann, K.P., Janssen,J.J., Baehr, W., Driessen, C.A., and Palczewski, K., 2006, Retinol dehydrogenase (RDH12) protects photoreceptors from light-induced degeneration in mice. J.Biol.Chem. 281:37697.PubMedCrossRefGoogle Scholar
  7. Mukherjee, P.K., Marcheselli, V.L., Serhan, C.N., and Bazan, N.G., 2004, Neuroprotectin D1: a docosahexaenoic acid-derived docosatriene protects human retinal pigment epithelial cells from oxidative stress. Proc. Natl. Acad. Sci. USA. 101:8491.PubMedCrossRefGoogle Scholar
  8. Tan, E., Ding, X.Q., Saadi, A., Agarwal, N., Naash, M.I., and Al-Ubaidi, M.R., 2004, Expression of cone-photoreceptor-specific antigens in a cell line derived from retinal tumors in transgenic mice. Invest Ophthalmol. Vis. Sci. 45:764.PubMedCrossRefGoogle Scholar
  9. Tanito, M., Yoshida, Y., Kaidzu, S., Ohira, A., and Niki, E., 2006, Detection of lipid peroxidation in light-exposed mouse retina assessed by oxidative stress markers, total hydroxyoctadecadienoic acid and 8-iso-prostaglandin F2alpha. Neurosci. Lett. 398:63.PubMedCrossRefGoogle Scholar
  10. Tomany, S.C., Cruickshanks, K.J., Klein, R., Klein B.E., and Knudtson, M.D., 2004, Sunlight and the 10-year incidence of age-related maculopathy: the beaver dam eye study. Arch. Ophthalmol. 122:750.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yogita Kanan
    • 1
  • Anne Kasus Jacobi
    • 2
  • Kjell Sawyer
  • David S. Mannel
    • 2
  • Joyce Tombran Tink
    • 3
  • Muayyad R. Al-Ubaidi
    • 4
  1. 1.Department of Cell BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Department of OphthalmologyDean McGee Eye InstituteOklahoma CityUSA
  3. 3.Department of Ophthalmology and Visual ScienceYale University School of MedicineNew Haven
  4. 4.Department of Cell BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

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